| Rjkk Properties Limited | Real estate | 2021 |
| RJM & Sons (Scotland) Limited | Manufacturing | 2002 |
| Rkmtransports Limited | Transport & logistics | 2019 |
| RL Kids Fitness Ltd | Arts & recreation | 2023 |
| RLA Media Limited | Information & communication | 2023 |
| RM Recycling Ltd | Water & waste | 2010 |
| Robert Bosch Limited | Manufacturing | 1924 |
| Robert Miller (Denny) Limited | Construction | 2015 |
| Robertson Project Management (Fife) Ltd | Construction | 2011 |
| Robinson Morris Limited | Administrative & support | 2016 |
| Roca Rentals Limited | Real estate | 2026 |
| Rohan Properties Scotland Limited | Real estate | 2019 |
| Rokkit Ltd | Information & communication | 2025 |
| Roks Holdings Limited | Real estate | 2026 |
| Rollando Limited | Wholesale & retail | 2024 |
| Romanx Transport Ltd | Transport & logistics | 2026 |
| RON'S Guitar Tuition Ltd | Education | 2026 |
| Ron's Guitar Tuition Ltd | Education | 2026 |
| Rooftechcare Ltd | Construction | 2012 |
| Roros Food Ltd | Hospitality | 2022 |
| Rosewell Ventures Ltd. | Construction | 2019 |
| Ross Friel Motors Ltd | Wholesale & retail | 2024 |
| Rotawave Scotland Limited | Professional & scientific | 2015 |
| Rothes Draughting Ltd | Professional & scientific | 2024 |
| Rothes Properties Ltd | Real estate | 2021 |
| Rothes PUB Company Limited | Hospitality | 2018 |
| Rovsup Ltd | Professional & scientific | 2016 |
| ROW Property Lettings Limited | Real estate | 2025 |
| Rowan Group Limited | Wholesale & retail | 1978 |
| RR Property Scotland Ltd | Construction | 2026 |
| RS Garden Landscaping Ltd | Administrative & support | 2026 |
| RSF Inspection Ltd | Professional & scientific | 2025 |
| RSG Construction Limited | Construction | 2014 |
| RSG Rope Access Limited | Professional & scientific | 2023 |
| RTS Manufacturing Ltd | Extraterritorial | 1992 |
| Run Eight Ltd | Manufacturing | 2026 |
| Ruths RUG Rats Ltd | Arts & recreation | 2023 |
| S & H (Fife) Ltd | Wholesale & retail | 2012 |
| S & S Building and Landscaping Limited | Construction | 2025 |
| S a a Installations Ltd | Construction | 2019 |
| S J Roofing Fife Ltd | Construction | 2007 |
| S T GAS Services (Fife) Ltd | Construction | 2020 |
| S W Inspections Services Limited | Professional & scientific | 2025 |
| S&C Ventures Limited | Wholesale & retail | 2021 |
| S&K Dhillon Property Ltd | Real estate | 2024 |
| S&N Melville Properties Limited | Real estate | 2025 |
| S&S Bell Joinery Limited | Construction | 2018 |
| Sa-Line Logistics Ltd | Transport & logistics | 2019 |
| Sabre Assessing Scotland Ltd | Other services | 2025 |
| SAK Stores Ltd | Wholesale & retail | 2025 |
| Sakarya 1 Ltd | Hospitality | 2026 |
| Saltire Light Haulage Ltd | Transport & logistics | 2024 |
| Saltire Shipping Limited | Transport & logistics | 1998 |
| Salus Primum Limited | Professional & scientific | 2023 |
| Sandf (Grangemouth) Limited | Wholesale & retail | 2004 |
| Sara Kirkham Hairdressing Ltd | Other services | 2014 |
| Sarah Lock Leadership Limited | Education | 2017 |
| Sasgo Limited | Wholesale & retail | 2013 |
| Savage Kitchen Ltd | Manufacturing | 2019 |
| SBR Paving Ltd | Administrative & support | 2025 |
| SC1 Trading Limited | Real estate | 2026 |
| Scale Torc Limited | Administrative & support | 2021 |
| Scandic Limited | Manufacturing | 2016 |
| SCE Engineering Limited | Manufacturing | 2007 |
| Scentsual Ltd | Wholesale & retail | 2025 |
| Scentzora Ltd | Wholesale & retail | 2026 |
| Scoonie Tavern 2026 Limited | Hospitality | 2026 |
| Scotar Group Limited | Financial services | 2021 |
| Scotenergy Limited | Construction | 2006 |
| Scotrad Services Limited | Manufacturing | 2007 |
| Scotstar Resin Limited | Construction | 2019 |
| Scott & Simpson (Painters) Limited | Construction | 1977 |
| Scott Robertson Joiners Ltd | Construction | 2017 |
| Scottish Darts Association | Arts & recreation | 1987 |
| Scotwell Ltd. | Construction | 2018 |
| Scrap a Car Holdings Limited | Financial services | 2016 |
| Sdla Ltd | Information & communication | 2013 |
| Seeking Simplicity Limited | Extraterritorial | 2008 |
| Seilbhxproperty Ltd | Real estate | 2026 |
| Seiscon Limited | Mining & quarrying | 2006 |
| Select Automotive Fife Ltd | Wholesale & retail | 2025 |
| Selfmon Ltd | Administrative & support | 2017 |
| Semefab Limited | Manufacturing | 1986 |
| Sexy Bills Ltd | Professional & scientific | 2025 |
| SF Motorcycles Ltd | Wholesale & retail | 2025 |
| SF Property Company Limited | Real estate | 1999 |
| SF Technologies Limited | Information & communication | 2025 |
| SF UK Electrical Ltd | Construction | 2025 |
| Sfdraw Ltd | Wholesale & retail | 2024 |
| SG Technical Services Ltd | Manufacturing | 2012 |
| SH Joinery Fife Limited | Construction | 2020 |
| Shadow Blinds Limited | Manufacturing | 1988 |
| Sharks Groundworks Ltd | Construction | 2025 |
| Sharp Plumbing - Heating Ltd | Construction | 2025 |
| Shields Activated Learning Ltd | Education | 2023 |
| Shoppily Limited | Wholesale & retail | 2026 |
| Shotts Dental and Implant Centre Ltd | Health & social care | 2026 |
| Sidon Water Ltd | Manufacturing | 2019 |
| Sign Systems (Scotland) Ltd. | Manufacturing | 1999 |
| Silvanus Limited | Transport & logistics | 2003 |