| Tartan Vitalis Limited | Wholesale & retail | 2023 |
| Taurus Property Services Limited | Real estate | 2022 |
| Tayview Management Services Ltd | Professional & scientific | 2015 |
| TCM Solutions Limited | Information & communication | 1996 |
| Team Challenge Company Limited | Other services | 2012 |
| Technatro Ltd | Information & communication | 2020 |
| Teesave African Food Hub Ltd | Hospitality | 2026 |
| Temp Van Cover Ltd | Administrative & support | 2010 |
| Tenebris Prints Limited | Wholesale & retail | 2025 |
| Textile Care Supplies (Holdings) Limited | Wholesale & retail | 2016 |
| Textile Care Supplies Ltd. | Wholesale & retail | 1999 |
| THE Brewistan Ltd | Wholesale & retail | 2026 |
| THE Buffalo Farm Produce Ltd | Agriculture & fishing | 2024 |
| THE Chubby PT - Haulage and Training Services Ltd | Transport & logistics | 2025 |
| THE Crown Leven Ltd | Hospitality | 2025 |
| THE Fife Herbal Dispensary Limited | Health & social care | 2005 |
| THE GBC Bridge Limited | Wholesale & retail | 2019 |
| THE Girl in the Kings Arms Ltd | Information & communication | 2017 |
| THE Golf Hotel ST Andrews Ltd | Hospitality | 2019 |
| THE Good - Kirkcaldy Ltd | Real estate | 2025 |
| THE Good Digitals Ltd | Information & communication | 2025 |
| THE Good Events Company (Scotland) Ltd | Administrative & support | 2025 |
| THE Invincible Press Ltd | Manufacturing | 2016 |
| THE Jkdaws Ltd | Hospitality | 2024 |
| THE Kirkcaldy KOP Ltd | Hospitality | 2025 |
| THE Loveoliver Shop Limited | Wholesale & retail | 2022 |
| THE LT Brows Project Limited | Education | 2024 |
| THE Luib Hotel and Country Club Limited | Hospitality | 2025 |
| THE Muirhead Outreach Project Limited | Health & social care | 2004 |
| THE Real David Cameron Limited | Professional & scientific | 2009 |
| THE Roof Doctor (Fife) Limited | Construction | 2025 |
| THE Sandwich Shop Glenrothes Limited | Hospitality | 2017 |
| THE Station Inn (Glengarnock) Ltd | Hospitality | 2023 |
| THE Transactional Analysis Association (Scotland) | Education | 2010 |
| THE Twin Shock Scramble Club (Scotland) Limited | Arts & recreation | 1996 |
| THE Venue @ Duntarvie Castle Ltd | Hospitality | 2025 |
| THE Venue Perth Limited | Hospitality | 2017 |
| THE Vettriano Trust | Arts & recreation | 2002 |
| THE WEE Motor Company Ltd | Wholesale & retail | 2025 |
| THE WEE Thistle Sleep Consultant Ltd | Health & social care | 2025 |
| Theycare Limited | Information & communication | 2024 |
| Third Angle Projection Limited | Professional & scientific | 2014 |
| Thistle Ground Maintenance Ltd. | Administrative & support | 2001 |
| Thomson Supplies Ltd | Wholesale & retail | 2024 |
| Thunder Holdings Limited | Professional & scientific | 2020 |
| Thunder Leisure Ltd | Hospitality | 2004 |
| Tiger Lily’S Chinese Takeaway Ltd | Hospitality | 2025 |
| Tiki-Taka Football School Ltd | Education | 2019 |
| Tilepal Limited | Wholesale & retail | 2019 |
| Timber Garden Buildings (Scotland) Limited | Manufacturing | 2010 |
| TIMBERCUT4U Limited | Extraterritorial | 2008 |
| Tite-Lite (UK) Limited | Manufacturing | 2005 |
| TJ Cleaning Services Ltd | Administrative & support | 2025 |
| TLS Hydro Power Limited | Energy | 1999 |
| TO Travel Limited | Administrative & support | 2026 |
| Tone Research Ltd | Professional & scientific | 2026 |
| Topographica Ltd | Professional & scientific | 2019 |
| Touchdown at the Madhouse Coffee Shop Ltd | Hospitality | 2025 |
| Town Cabs Glenrothes Ltd | Transport & logistics | 2024 |
| TPH Plumbing and Heating Limited | Construction | 2015 |
| Transform Development Limited | Wholesale & retail | 2003 |
| Transmitair Limited | Information & communication | 2020 |
| Transy Properties Limited | Real estate | 2022 |
| Trend Technologies Scotland Limited | Manufacturing | 2004 |
| Triple M Industrial Roofing Ltd | Construction | 2023 |
| Tsaj Ltd | Hospitality | 2022 |
| Turbanhaven Ltd | Manufacturing | 2026 |
| Turf Titans Ltd | Extraterritorial | 2025 |
| Tykon International Ltd | Wholesale & retail | 2026 |
| Tyreflex Scotland Ltd | Wholesale & retail | 2026 |
| UCC Consultants Limited | Information & communication | 2012 |
| Ultimate Property Solutions (Fife) Limited | Construction | 2021 |
| Ultratherm Limited | Manufacturing | 1994 |
| UNITSIX8 Autodesign Ltd | Wholesale & retail | 2021 |
| Utilico Ltd | Construction | 2018 |
| Utility ROV Services (Holdings) Limited | Real estate | 2016 |
| Utility ROV Services (S3) Limited | Manufacturing | 2016 |
| UW Global Ltd | Wholesale & retail | 2026 |
| V9 Enterprises Limited | Construction | 2020 |
| Valentins Lettings (Fife) Limited | Real estate | 2023 |
| Valeting Centre Glenrothes Ltd | Administrative & support | 2022 |
| Van Load Removals UK Ltd | Transport & logistics | 2025 |
| View Mauritius Properties Limited | Wholesale & retail | 2017 |
| View Spanish Properties Limited | Real estate | 2013 |
| Vivid NDT and Access Ltd | Professional & scientific | 2020 |
| Voda-Bone Pet Supplies Limited | Wholesale & retail | 2018 |
| Volpe & Castello (Distribution) Limited | Wholesale & retail | 2025 |
| Volpecastello Limited | Wholesale & retail | 2022 |
| Vorsehen Trades Ltd | Wholesale & retail | 2021 |
| W Clarke NDT Ltd | Professional & scientific | 2025 |
| W J Electrical Supplies Ltd. | Administrative & support | 2003 |
| W Mcmillan Foods Ltd | Manufacturing | 2018 |
| W R Consultancy (Scotland) Ltd | Administrative & support | 2014 |
| Walk on Wood Ltd | Wholesale & retail | 2019 |
| Wallace Roofing and Building Limited | Construction | 1999 |
| Wasp Renewable Services Ltd | Public administration | 2025 |
| WBS Motors Limited | Wholesale & retail | 2007 |
| WE FIX Gearboxes Ltd | Wholesale & retail | 2019 |
| Web Design Pro Ltd | Information & communication | 2024 |
| West END Lawn Tennis Club Limited | Arts & recreation | 2011 |