| East on Projects Ltd | Construction | 2013 |
| East on Projects Trustee Limited | Extraterritorial | 2023 |
| East Preston Dental Clinic Limited | Health & social care | 2011 |
| East Print Limited | Manufacturing | 2004 |
| East Street Health Shop Ltd | Wholesale & retail | 2025 |
| East Sussex Catering Ltd | Hospitality | 2009 |
| East Sussex Community Voice CIC | Information & communication | 2012 |
| East Sussex Fibreglass Limited | Other services | 2007 |
| East Sussex Game Farm Limited | Agriculture & fishing | 2023 |
| East Sussex General Practitioner Services Ltd | Health & social care | 2024 |
| East Sussex Gynaecology Limited | Health & social care | 2018 |
| East Sussex Hearing | Information & communication | 2003 |
| East Sussex Hospitality Ltd | Hospitality | 2025 |
| East Sussex Property Investment Ltd | Real estate | 2025 |
| East Sussex Property Management Services Ltd | Real estate | 2025 |
| East Sussex Vision Care | Health & social care | 1998 |
| East West Refurbishment Limited | Construction | 2003 |
| Eastbach Limited | Financial services | 2005 |
| Eastborn Limited | Hospitality | 2016 |
| Eastbourne & District Mencap Limited | Health & social care | 2007 |
| Eastbourne ABC | Arts & recreation | 2014 |
| Eastbourne Accountants Ltd | Extraterritorial | 2021 |
| Eastbourne Alarm Maintenance Limited | Manufacturing | 2012 |
| Eastbourne and District Enterprise Agency Limited | Other services | 1983 |
| Eastbourne and District Indoor Bowls Association Limited | Arts & recreation | 1977 |
| Eastbourne and District Veterans CIC | Administrative & support | 2023 |
| Eastbourne and RAM Motor Club Limited | Arts & recreation | 1957 |
| Eastbourne Area Parents' Action Group (Learning Disabilities) | Education | 1995 |
| Eastbourne Auctions Ltd | Administrative & support | 2012 |
| Eastbourne Barbers Limited | Other services | 2016 |
| Eastbourne Best Bar None CIC | Hospitality | 2024 |
| Eastbourne Borough Football Club 2023 Limited | Arts & recreation | 2023 |
| Eastbourne Borough Football Club CIC | Arts & recreation | 2008 |
| Eastbourne Cafe Ltd | Hospitality | 2024 |
| Eastbourne Car Chargers Ltd | Construction | 2021 |
| Eastbourne Carnival CIC | Education | 2024 |
| Eastbourne Catering Limited | Hospitality | 2025 |
| Eastbourne Churches Visiting | Health & social care | 2016 |
| Eastbourne Citizens Advice Bureau | Other services | 2001 |
| Eastbourne Coach Finishers Limited | Wholesale & retail | 1957 |
| Eastbourne Coaches Limited | Transport & logistics | 2023 |
| Eastbourne Cocktail Club Ltd | Hospitality | 2023 |
| Eastbourne College (Incorporated) | Education | 1911 |
| Eastbourne Community Services Ltd | Transport & logistics | 2021 |
| Eastbourne Complementary Health Center Ltd | Health & social care | 2015 |
| Eastbourne Construction Ltd | Construction | 2018 |
| Eastbourne Doggy Daycare Ltd | Agriculture & fishing | 2024 |
| Eastbourne Extensions and Conversions Ltd | Construction | 2020 |
| Eastbourne Food Partnership CIC | Hospitality | 2021 |
| Eastbourne Foodbank | Other services | 2012 |
| Eastbourne Foods Ltd | Hospitality | 2024 |
| Eastbourne Garages Limited | Wholesale & retail | 2006 |
| Eastbourne Grillings Ltd | Hospitality | 2025 |
| Eastbourne Hand Car Wash Limited | Administrative & support | 2019 |
| Eastbourne Healthcare Partnership Limited | Health & social care | 2007 |
| Eastbourne Holiday Rentals Ltd | Hospitality | 2026 |
| Eastbourne Homes Limited | Real estate | 2005 |
| Eastbourne Hot Food Ltd | Hospitality | 2024 |
| Eastbourne Housing Investment Company Limited | Real estate | 2015 |
| Eastbourne International Food Centre Ltd | Wholesale & retail | 2020 |
| Eastbourne Investments Ltd | Administrative & support | 2010 |
| Eastbourne Lammas Festival Ltd | Other services | 2026 |
| Eastbourne Launderette Ltd | Other services | 2021 |
| Eastbourne Lettings Limited | Real estate | 2007 |
| Eastbourne Management Ltd | Professional & scientific | 2021 |
| Eastbourne Member Ltd | Hospitality | 2025 |
| Eastbourne Mini Market Ltd | Wholesale & retail | 2025 |
| Eastbourne Motoring Centre Limited | Wholesale & retail | 1988 |
| Eastbourne Neptunes Basketball Club CIC | Arts & recreation | 2023 |
| Eastbourne Performing Arts Studio Limited | Arts & recreation | 2025 |
| Eastbourne Pizza Ltd | Hospitality | 2025 |
| Eastbourne Podiatry Ltd | Health & social care | 2008 |
| Eastbourne Productions Limited | Professional & scientific | 2021 |
| Eastbourne Properties Limited | Real estate | 2004 |
| Eastbourne Property Rentals Ltd | Real estate | 2018 |
| Eastbourne Psychotherapy Limited | Professional & scientific | 2019 |
| Eastbourne Pump Company Limited | Manufacturing | 2019 |
| Eastbourne Reisen Ltd | Administrative & support | 2022 |
| Eastbourne Reporter Community Interest Company | Information & communication | 2022 |
| Eastbourne Riviera Ltd | Hospitality | 2008 |
| Eastbourne Roofing Contractors Ltd | Construction | 2022 |
| Eastbourne Scaffolding Limited | Construction | 2025 |
| Eastbourne Shutters Ltd | Wholesale & retail | 2020 |
| Eastbourne Spice Ltd | Hospitality | 2026 |
| Eastbourne Stagers CIC | Arts & recreation | 2025 |
| Eastbourne Store Ltd | Wholesale & retail | 2024 |
| Eastbourne Studio Pottery C.I.C. | Arts & recreation | 2016 |
| Eastbourne Surveyors Limited | Real estate | 2012 |
| Eastbourne Sweets Limited | Hospitality | 2025 |
| Eastbourne Taxi Hackney Ltd | Transport & logistics | 2025 |
| Eastbourne Taxis Ltd | Transport & logistics | 2023 |
| Eastbourne Theatres Limited | Arts & recreation | 2016 |
| Eastbourne Training Complex Limited | Arts & recreation | 2014 |
| Eastbourne Trampoline Limited | Arts & recreation | 2024 |
| Eastbourne Tyres Auto Centre Limited | Wholesale & retail | 2022 |
| Eastfield Sporting Goods Company Limited | Wholesale & retail | 2015 |
| Eastfox Marketing Limited | Professional & scientific | 2023 |
| Eastgate (Kent) Development Ltd | Construction | 2010 |
| Eastgate (Kent) Ltd | Other services | 2004 |
| Eastings Property Limited | Real estate | 2016 |