| DR Sara Mccluskey Limited | Administrative & support | 2010 |
| DR Senna Clinic Limited | Health & social care | 2023 |
| DR Sophia Amjad Ltd | Health & social care | 2025 |
| DR Tempest Consultancy Ltd | Health & social care | 2022 |
| DR. Quiroga Histopathology Services Ltd | Health & social care | 2015 |
| Draft Stables Ltd | Professional & scientific | 2021 |
| Drake Pharmaceuticals Limited | Manufacturing | 2021 |
| Drake's Extracts Ltd | Manufacturing | 2017 |
| DRC Mechanical Ltd | Professional & scientific | 2018 |
| Drdk Ltd | Health & social care | 2024 |
| Dream Home Construction Sussex Ltd | Construction | 2026 |
| Drewstead Road 56 Limited | Households | 2016 |
| Drgn Clothing Ltd | Wholesale & retail | 2026 |
| Drivenbydetail Ltd | Wholesale & retail | 2025 |
| Driving Classics Ltd | Wholesale & retail | 2015 |
| Driving Force Logistics Ltd | Transport & logistics | 2012 |
| Drmod Ltd | Information & communication | 2025 |
| Drublik Limited | Hospitality | 2025 |
| DS Consultancy Management Ltd | Wholesale & retail | 2020 |
| DS1 Consultancy Ltd | Administrative & support | 2022 |
| DSB Orthodontics Limited | Real estate | 2021 |
| Dsga Properties Limited | Real estate | 2021 |
| DSP Capital Ventures Ltd | Financial services | 2026 |
| DSP Consulting Group Ltd | Information & communication | 2019 |
| DST Services(Gb) Ltd | Professional & scientific | 2010 |
| DTA Residential Limited | Other services | 2018 |
| DTB Cars Ltd | Wholesale & retail | 2025 |
| Dtsb Holdings Limited | Financial services | 2011 |
| DTY (Brighton) Limited | Hospitality | 2013 |
| Dubai B&S Barbers Ltd | Other services | 2026 |
| Ducere Consulting Limited | Professional & scientific | 2011 |
| Duckies Digital Limited | Information & communication | 2025 |
| Dudles Digital Ltd | Professional & scientific | 2025 |
| Dudley Venture Holdings Ltd | Real estate | 2020 |
| Duka Construction Ltd | Construction | 2014 |
| Duke Group Ltd | Financial services | 2022 |
| Duke of UKE Tuition Limited | Education | 2013 |
| Duke Retail Ltd | Wholesale & retail | 2022 |
| Duke Villas Property Company Limited | Households | 2006 |
| Dunamis Therapy Hub Limited | Health & social care | 2018 |
| Dunkery Ventures Ltd | Professional & scientific | 2026 |
| Dunkgate Consulting Limited | Professional & scientific | 2020 |
| Dunne Orthodontics Limited | Health & social care | 2024 |
| Dunvegan (Eastbourne) Limited | Hospitality | 2007 |
| Durlden Maintenance Co Limited | Real estate | 1958 |
| Durrant Property Development Limited | Real estate | 2024 |
| Dust TIL Dawn Cleaning Ltd | Administrative & support | 2025 |
| Duty Management & Trade Solutions Ltd | Professional & scientific | 2023 |
| Duvacourt Limited | Construction | 2003 |
| DV Digital Assets Ltd | Financial services | 2026 |
| DVB Residential Limited | Real estate | 2018 |
| Dwell Finance Ltd | Real estate | 2020 |
| DX Construction Limited | Construction | 2021 |
| DYC Investments Limited | Real estate | 2024 |
| Dylan Miles Ltd | Wholesale & retail | 2017 |
| Dynamic Beauty Academy Eastbourne Ltd | Other services | 2025 |
| Dynamic Engineering Projects Ltd | Construction | 2011 |
| Dynamic Systems (UK) Limited | Financial services | 2013 |
| Dynamic Systems ID Limited | Manufacturing | 1987 |
| Dynamic Tech Ltd | Information & communication | 2020 |
| Dyndev Ltd | Professional & scientific | 2026 |
| Dynoto Ltd | Wholesale & retail | 2025 |
| Décolletage Ltd | Manufacturing | 2026 |
| E & G Ortho Limited | Health & social care | 2011 |
| E B Prodec Limited | Construction | 2019 |
| E Store Hub Ltd | Wholesale & retail | 2025 |
| E Top Construction Ltd | Construction | 2023 |
| E&K Enterprises Limited | Wholesale & retail | 2022 |
| E&N Homes Limited | Real estate | 2020 |
| E-Marketing Direct Limited | Information & communication | 2017 |
| E-Vat Services Ltd | Administrative & support | 2018 |
| E3 Events Limited | Arts & recreation | 2010 |
| E3 Sports Events Ltd | Arts & recreation | 2023 |
| E3HEALTHCARE Limited | Health & social care | 2023 |
| E4 Corners Limited | Information & communication | 2026 |
| E5 Pubs (London) Ltd | Hospitality | 2022 |
| E8 Pubs (London) Ltd | Hospitality | 2023 |
| EA Marketing Group Ltd | Professional & scientific | 2026 |
| EAD Investments PTY Ltd | Real estate | 2018 |
| Eadean Ltd | Professional & scientific | 2023 |
| Eadie Investments Limited | Financial services | 2025 |
| Eadm Ltd | Wholesale & retail | 2018 |
| EAG Marketing Ltd | Financial services | 2011 |
| Eagles High Rock Ltd | Health & social care | 2023 |
| EAR Clarity Ltd | Health & social care | 2025 |
| Earley Dental Practice Limited | Health & social care | 2024 |
| Earls Court Dental Ltd | Health & social care | 2018 |
| Early Beginnings Nurseries Limited | Education | 2012 |
| Early Oak Reproductions Limited | Manufacturing | 2011 |
| Early Rise Care Ltd | Health & social care | 2024 |
| Earth Academy Records Limited | Arts & recreation | 2006 |
| Earth Station Systems Limited | Manufacturing | 2024 |
| Earthily Limited | Real estate | 2021 |
| Earthstone Homes HBL Ltd | Real estate | 2021 |
| Easing Care Ltd | Health & social care | 2024 |
| East Block Martello Quay Residents Company Limited | Households | 2003 |
| East Dean and Sheepscombe Landscaping and Garden Buildings Ltd | Construction | 2023 |
| East Dean Downlands (Roads) Limited | Other services | 1984 |
| East Dean Greensward Limited | Extraterritorial | 2012 |
| East on Facilities Management Ltd | Professional & scientific | 2023 |