| DHC Group Limited | Administrative & support | 2026 |
| Dighton Properties Limited | Real estate | 2023 |
| Divine Mercy Accountant's Limited | Professional & scientific | 2025 |
| DJY Plant and Commercials Ltd | Manufacturing | 2025 |
| Dojg Ltd | Manufacturing | 2019 |
| Dominator Property Services Limited | Administrative & support | 2017 |
| Domli Limited | Wholesale & retail | 2020 |
| DR Iliyan Bogolyubov Limited | Health & social care | 2025 |
| Draycott Builders Limited | Construction | 2022 |
| Draycott Projects Ltd | Professional & scientific | 2024 |
| Dream Start Developments Ltd | Administrative & support | 2024 |
| DTE Technologies Limited | Information & communication | 2024 |
| DTS Electronics Ltd | Manufacturing | 2022 |
| Dubtrubs Ltd | Administrative & support | 2019 |
| Dursley Court Flat Management Company Limited | Households | 1994 |
| Dursley Homecare Limited | Real estate | 1981 |
| Dursley Hotel Limited | Hospitality | 2016 |
| Dursley Kurdish Barber Limited | Other services | 2026 |
| Dursley Minnows Rugby Limited | Arts & recreation | 2023 |
| Dursley OUT of School Club Limited | Health & social care | 1997 |
| Dursley Town Sports Club Limited | Arts & recreation | 2018 |
| Durzles Ltd | Hospitality | 2026 |
| E and W Holdings Limited | Financial services | 2017 |
| E M Trusted Builders Ltd | Manufacturing | 2016 |
| Eagle EYE Surgical Limited | Information & communication | 2020 |
| Eastbound Consulting Limited | Construction | 2023 |
| Eastface Technology Limited | Information & communication | 2012 |
| Easy Contracting Ltd | Professional & scientific | 2024 |
| Echo South Holdings Limited | Financial services | 2024 |
| Ecoskye Ltd | Construction | 2020 |
| Eddie Ratcliffe Transport Ltd | Transport & logistics | 2015 |
| Edge Flat Roofing Ltd | Construction | 2025 |
| Edge Therapy Rooms Limited | Other services | 2023 |
| Editor Software (UK) Limited | Information & communication | 1989 |
| Effortec Limited | Manufacturing | 2000 |
| EGG Lenses Ltd | Wholesale & retail | 2023 |
| Eits Limited | Information & communication | 2002 |
| Elaine Freeman Therapies Ltd | Health & social care | 2017 |
| Elcombe Firewood Limited | Agriculture & fishing | 2008 |
| Eldraw Estates Limited | Real estate | 2024 |
| Elevation One Building Design Ltd | Professional & scientific | 2007 |
| Elizabeth ELY Landscape and Garden Design Limited | Other services | 2018 |
| Emerald Reupholstery Limited | Other services | 2015 |
| Emergency Response Systems Ltd | Health & social care | 2005 |
| Empowered Space for People Ltd | Information & communication | 2016 |
| Emre Auto Repair Ltd | Wholesale & retail | 2021 |
| Endurance Machine Knives Ltd. | Manufacturing | 1997 |
| Energy Business Intelligence Ltd. | Manufacturing | 2020 |
| Erious Ltd | Wholesale & retail | 2021 |
| Everbuild Scaffolding Limited | Construction | 2025 |
| Everhot Holdings Limited | Financial services | 2017 |
| Everhot Ltd. | Manufacturing | 1998 |
| Everhot Property Limited | Real estate | 2017 |
| Expedia Property Development Limited | Construction | 2004 |
| F Bailey & SON Limited | Professional & scientific | 1997 |
| Factory First (South West) Limited | Wholesale & retail | 2010 |
| Facts 4 Life Limited | Education | 2012 |
| FACTS4LIFE Education CIC | Professional & scientific | 2022 |
| Faded Grandeur Limited | Extraterritorial | 2023 |
| Fairview Battery Storage Limited | Construction | 2021 |
| Family Hair Ltd | Other services | 2024 |
| Faulds Dimensional Control Ltd | Professional & scientific | 2026 |
| Faye Lewis Projects Ltd | Professional & scientific | 2026 |
| Fiery Lane Ltd | Arts & recreation | 2015 |
| Finch Finance Ltd | Education | 2021 |
| Fire Alarms Maintained Ltd | Manufacturing | 2013 |
| Fireproof Media Ltd | Professional & scientific | 2014 |
| First Glass Dursley Ltd | Construction | 2014 |
| Five Little Limited | Hospitality | 2026 |
| Fivetone Cabinetry Ltd | Manufacturing | 2026 |
| Fkcp Consulting Limited | Real estate | 2022 |
| Flatlet Dwellings (Dursley) Limited | Real estate | 1963 |
| Flexy Care Limited | Administrative & support | 2018 |
| Flickini Ltd | Wholesale & retail | 2018 |
| Flourish Vale Community Care Ltd | Health & social care | 2026 |
| Footco Limited | Construction | 2003 |
| Forging People Ltd | Health & social care | 2020 |
| Forthay Granola Limited | Manufacturing | 2023 |
| Forthergill Limited | Information & communication | 1999 |
| Fortlox Security Ltd | Administrative & support | 2024 |
| Foxes Meadow Management Company Limited | Households | 2018 |
| Free Range Creativity | Education | 2014 |
| Friog Caravan Park Limited | Professional & scientific | 1977 |
| Frome Printing Company Ltd | Manufacturing | 2007 |
| Full Life Fitness Limited | Arts & recreation | 2014 |
| Further Communications Ltd | Education | 2025 |
| Futura Foods UK Limited | Wholesale & retail | 1993 |
| G & S Ashenford Limited | Administrative & support | 2004 |
| G Jones Boiler Services Ltd | Construction | 2010 |
| G Morse & SON Ltd | Construction | 2018 |
| Gebruder Schoeller Gesellschaft MIT Beschrankter Haftung | | 1992 |
| Gentle EAR Clinic Ltd | Health & social care | 2025 |
| Geometra Project Services Limited | Professional & scientific | 2023 |
| Geospatial Engineering Limited | Extraterritorial | 2022 |
| Gibbons Carpentry Limited | Manufacturing | 2025 |
| GL 11 Community Project Ltd | Other services | 2009 |
| Glam Aesthetics by Zoey Ltd | Other services | 2021 |
| Glasberg Ltd | Construction | 2016 |
| GLD Renovations Ltd | Construction | 2025 |
| Gloucester Professional Glass Repairs Limited | Manufacturing | 2019 |