| Gloucestershire Mountaineering Club Ltd | Arts & recreation | 2025 |
| GMN European Consultants Ltd | Professional & scientific | 2021 |
| Going OUT in Style Limited | Transport & logistics | 2024 |
| Golden Classic Vehicles Ltd | Transport & logistics | 2023 |
| Golden Goose Brewery Limited | Manufacturing | 2023 |
| Goldfish Accounting Solutions Limited | Professional & scientific | 2009 |
| Gorilla Property Co. Ltd | Hospitality | 2023 |
| Goring Limited | Financial services | 2023 |
| Grace Home Nepal | Professional & scientific | 2016 |
| Grahame's Hair Ltd | Other services | 2023 |
| GSG Property Investment Limited | Real estate | 2022 |
| GSG Property Solutions Limited | Real estate | 2022 |
| Guardian Angell Business Support Limited | Administrative & support | 2016 |
| H&B Market Limited | Wholesale & retail | 2024 |
| Hambro Marn Ltd | Professional & scientific | 2013 |
| Hardill Technologies Ltd | Information & communication | 2021 |
| Hardwood Furniture Online Ltd | Wholesale & retail | 2020 |
| Harrys Home Gardens Ltd | Administrative & support | 2020 |
| Hart Converting & Industrial Equipment Ltd | Wholesale & retail | 2009 |
| Havorn Limited | Professional & scientific | 2014 |
| Hawk Property Services Ltd | Real estate | 2022 |
| Heat Saviour Limited | Manufacturing | 2022 |
| Heliot Europe (UK) Limited | Information & communication | 2023 |
| Helmflux Ltd | Information & communication | 2026 |
| Hence Ltd. | Wholesale & retail | 2017 |
| Hernandez Frutos Limited | Financial services | 2020 |
| Hewitts Dursley Limited | Wholesale & retail | 2023 |
| HGB Engineering Limited | Professional & scientific | 2017 |
| Himal Food Ltd | Hospitality | 2021 |
| Home Run Films Ltd | Information & communication | 2022 |
| Home Sauna Co Ltd | Wholesale & retail | 2026 |
| Horfield Lettings Ltd | Real estate | 2025 |
| Horse Osteopath LLP | | 2020 |
| Hott Group Limited | Wholesale & retail | 2021 |
| Hungama Ltd | Hospitality | 2024 |
| HWH Engineering Limited | Financial services | 2012 |
| Hybrid Dwellings Ltd | Real estate | 2024 |
| Hydroseeding Limited | Professional & scientific | 2006 |
| I-Gen Consulting Limited | Professional & scientific | 2014 |
| IAN Pollard Consultancy Limited | Administrative & support | 2014 |
| Iexec Ltd | Administrative & support | 2020 |
| Igbo BU Igbo Gloucestershire Ltd | Education | 2019 |
| Ilmews Ltd | Professional & scientific | 2025 |
| Immersa EV Limited | Manufacturing | 2023 |
| Immersa Holdings Limited | Financial services | 2020 |
| Immersa Investments Limited | Manufacturing | 2019 |
| Immersa Limited | Professional & scientific | 2016 |
| Immersa OFF Grid Limited | Professional & scientific | 2018 |
| Immersa Sustainable Community (Stroud District) Limited | Professional & scientific | 2021 |
| Impact and Materials Dynamics Ltd | Professional & scientific | 2012 |
| Impact Gardening Limited | Other services | 2003 |
| Income and Assets Limited | Financial services | 2016 |
| IND Park Limited | Real estate | 2024 |
| Indago Instruments Ltd | Professional & scientific | 2016 |
| Indy's Construction Limited | Construction | 2022 |
| Inevitable Systems Ltd | Professional & scientific | 2023 |
| Innovartec Ltd | Professional & scientific | 2011 |
| Inside OUT Cleaning and Restoration Limited | Administrative & support | 2012 |
| Ippi Limited | Wholesale & retail | 2011 |
| Italian for Ginger Ltd | Hospitality | 2016 |
| J Evans Forestry Contracts Ltd | Agriculture & fishing | 2024 |
| J Hughes Ventures Ltd | Financial services | 2025 |
| J K Kirby Limited | Professional & scientific | 2023 |
| J S L (Rangeworthy) Limited | Hospitality | 2007 |
| J&R Developments (Stroud) Ltd | Construction | 2017 |
| J.O.D Heating Ltd | Professional & scientific | 2022 |
| Jane Louise Manning Ltd | Extraterritorial | 2022 |
| Jason Ford Active Health Clinic Ltd | Other services | 2006 |
| JAY Goes Global Ltd | Information & communication | 2025 |
| JCE Recruitment Ltd | Administrative & support | 2023 |
| JDL Engines Ltd | Manufacturing | 2024 |
| JO Chapman Property Sales Ltd | Real estate | 2025 |
| JP & MJ Enterprises Limited | Wholesale & retail | 2024 |
| JTB Electrical Glos Limited | Energy | 2020 |
| K Withers Cleaning Ltd | Administrative & support | 2015 |
| K-Inks Tattoo Ltd | Other services | 2023 |
| K2 Manuals Ltd | Information & communication | 2021 |
| Kaca Limited | Hospitality | 2024 |
| Kashop Ltd | Wholesale & retail | 2025 |
| Katmal Limited | Professional & scientific | 2014 |
| Katwab Limited | Information & communication | 2017 |
| Katy Evans Limited | Arts & recreation | 2025 |
| Katygpropertyinvestor Ltd | Financial services | 2024 |
| Kava Media Employee Ownership Trustee Limited | Professional & scientific | 2021 |
| Kava Media Limited | Information & communication | 2000 |
| KCA EOT Limited | Extraterritorial | 2021 |
| Kcmt Ltd | Hospitality | 2026 |
| Kevin Sheehan and Nathan Polley Ltd | Other services | 2016 |
| Keysecure Limited | Manufacturing | 1995 |
| Khonstruct Ltd | Construction | 2025 |
| Kingshill House Limited | Arts & recreation | 1996 |
| Kingsley House Kingsdown Limited | Households | 1994 |
| Kj&Sa Ltd | Professional & scientific | 2025 |
| Kleris (Dursley) Limited | Hospitality | 2025 |
| KNB Consulting Ltd | Professional & scientific | 2018 |
| Knowledge Activation Technologies Limited | Information & communication | 2000 |
| Knowledge Change Action Ltd | Information & communication | 2011 |
| Kontak Limited | Wholesale & retail | 2001 |
| Kope Healthcare Limited | Health & social care | 2023 |
| Kordula Carpets Limited | Construction | 2012 |