| Blueford Beef Company Limited | Manufacturing | 2020 |
| Bowe Accounting Limited | Professional & scientific | 2006 |
| Bowstraight Property Limited | Real estate | 2017 |
| Box Court Garden Plants Ltd. | Wholesale & retail | 2011 |
| Bradley - Inge Project Consulting Limited | Professional & scientific | 2011 |
| Bramblings Forest School CIC | Education | 2023 |
| Brand New Story Limited | Professional & scientific | 2020 |
| Braycare Ltd | Health & social care | 2024 |
| Breakheart Community Project Ltd | Arts & recreation | 2009 |
| Brinksworth Mechanised Tree Services Ltd | Agriculture & fishing | 2024 |
| Brunel Business Analysts Limited | Professional & scientific | 2012 |
| Bubblesocial Ltd | Information & communication | 2025 |
| Budgo Limited | Real estate | 2018 |
| Buongiorno Caffe Ltd | Hospitality | 2025 |
| Business Support Services (Gloucestershire) Limited | Professional & scientific | 2009 |
| Bywater UX Ltd | Information & communication | 2015 |
| C C & I Ltd | Manufacturing | 2005 |
| Cainscross House Residents' Association Limited | Households | 2002 |
| Cakemeister Productions Limited | Arts & recreation | 2018 |
| CAM Catering Butchers Limited | Wholesale & retail | 2002 |
| CAM DP Ltd | Health & social care | 2021 |
| CAM Family Butchers Ltd | Wholesale & retail | 2005 |
| CAM Green Land Ltd | Financial services | 2019 |
| CAM Sports Club | Arts & recreation | 2017 |
| CAM Systems Limited | Information & communication | 1990 |
| CAM Technical Services Ltd | Manufacturing | 2019 |
| Cantabgilly Ltd | Arts & recreation | 2010 |
| Cappinni's Pizza Ltd | Hospitality | 2018 |
| Caratti Sport Limited | Professional & scientific | 2002 |
| Care Performance Solutions Ltd | Information & communication | 2025 |
| Careerradar Limited | Information & communication | 2017 |
| Carpet & Vinyl Ltd | Construction | 2024 |
| Castle Financial Services Ltd | Financial services | 2002 |
| Castle Gate Dental Limited | Health & social care | 2008 |
| Catalix Limited | Professional & scientific | 2024 |
| Catenna Ltd | Information & communication | 2022 |
| Catherine Ward-Bexter Limited | Professional & scientific | 2026 |
| CB Premium Limited | Wholesale & retail | 2024 |
| Cbns Sales Ltd | Wholesale & retail | 2024 |
| CDL Events Ltd | Administrative & support | 2024 |
| CDS Partnership Limited | Professional & scientific | 2020 |
| Cedar Ridge Ltd | Professional & scientific | 2026 |
| Centaur Industries Ltd | Manufacturing | 2012 |
| CF Business Consulting Limited | Professional & scientific | 2021 |
| Change Plus Consultancy Limited | Professional & scientific | 2000 |
| Chapel Court (Dursley) Management Company Limited | Households | 1999 |
| Cheeseburger Ltd | Hospitality | 2025 |
| CHG Solutions Limited | Administrative & support | 2010 |
| China Chef (CAM) Ltd | Hospitality | 2014 |
| Chpc Digital Ltd | Information & communication | 2018 |
| City Walls Art Ltd | Wholesale & retail | 2026 |
| Clare Williams Research Limited | Professional & scientific | 2003 |
| Classic Barbershop and Salon Ltd | Other services | 2022 |
| Clbudge Ltd | Professional & scientific | 2024 |
| Clem Agri Ltd | Wholesale & retail | 2025 |
| Clip & Collect Dog Grooming Ltd | Other services | 2023 |
| Clovergate Construction Ltd | Construction | 2017 |
| Clt-Group Limited | Professional & scientific | 2016 |
| Colin Higgins Group Holdings Limited | Hospitality | 2010 |
| COLLABAR8 Limited | Hospitality | 2023 |
| Construction & Design Limited | Professional & scientific | 2003 |
| Controls Engineering Services Ltd | Professional & scientific | 2023 |
| Corner Farm Enterprise Ltd | Hospitality | 2021 |
| Cornerstore Studio Ltd | Professional & scientific | 2025 |
| Cotswold Barkery Ltd | Wholesale & retail | 2026 |
| Cotswold Fast Foods Ltd | Hospitality | 2016 |
| Cotswold Manor Collection Ltd | Wholesale & retail | 2025 |
| Cotswold Medical Supplies Ltd | Wholesale & retail | 2025 |
| Cotswold Rehabilitation Services Ltd | Health & social care | 2012 |
| Cotswold Rendering Designs Ltd | Construction | 2025 |
| Cotswold Treatments Ltd | Construction | 2019 |
| Cotswold Vale Planning Ltd | Professional & scientific | 2021 |
| Cotswold Valley One Loft Race Limited | Agriculture & fishing | 2021 |
| Courtyard Clinic (Dursley) Ltd | Health & social care | 2003 |
| CPL Awareness CIC | Education | 2025 |
| Creative Dreaming Ltd | Information & communication | 1999 |
| Crisp Growth Consulting (CGC) UK Ltd | Professional & scientific | 2025 |
| Crock's Gym Limited | Arts & recreation | 2019 |
| Cross Country Medical Services Limited | Health & social care | 2014 |
| Crypsis Ltd | Professional & scientific | 2026 |
| Culinary Longhouse Ltd | Hospitality | 2025 |
| Cullen Group Limited | Other services | 1997 |
| Curiosity Research Ltd | Professional & scientific | 2023 |
| Currer Web Consulting Limited | Information & communication | 2007 |
| Custom Consulting Services Ltd | Professional & scientific | 2006 |
| Customs Management Bureaux Limited | Administrative & support | 2015 |
| D R Howse Services Ltd | Administrative & support | 2011 |
| D W T Farrier Ltd. | Administrative & support | 2011 |
| Damp Detectives Oxford and Swindon Ltd | Professional & scientific | 2024 |
| Daniell Consulting Limited | Professional & scientific | 2017 |
| Darton Holdings Ltd | Professional & scientific | 2024 |
| Dasion Fabrication Limited | Manufacturing | 2007 |
| Davies Torres Design Ltd | Professional & scientific | 2011 |
| Dbfibre Ltd | Information & communication | 2020 |
| Decor Rent PT Ltd | Administrative & support | 2024 |
| Deliver 4 U Ltd | Transport & logistics | 2014 |
| Design IS Good Limited | Other services | 2013 |
| Desirabuild Ltd | Construction | 2019 |
| DEV Installations Ltd | Construction | 2020 |
| DGL Consulting Ltd | Other services | 2018 |