| Elliott Clark Flowers Ltd. | Wholesale & retail | 2003 |
| Elmca Design Engineering Ltd | Professional & scientific | 2013 |
| Elmca Property Holdings Ltd | Real estate | 2022 |
| Elmwood Homes Limited | Construction | 2019 |
| EM Electrical Solutions Ltd | Construction | 2020 |
| Embracing Life CIC | Health & social care | 2020 |
| EMC Letting Ltd | Real estate | 2021 |
| Emdg Holdings Ltd | Real estate | 2021 |
| Emerald Utility Services Ltd | Construction | 2023 |
| EMP1RE Ltd | Real estate | 2022 |
| Empire Commercial Lending Ltd | Financial services | 2025 |
| Empire Mortgages Ltd | Financial services | 2016 |
| Employment Resolved Ltd | Administrative & support | 2025 |
| Emporium Cart Ltd | Wholesale & retail | 2024 |
| Emranicandles Ltd | Extraterritorial | 2023 |
| Endako Relocations Limited | Hospitality | 2022 |
| Endeavour Works Limited | Information & communication | 2020 |
| Engine 1991 Creations Ltd | Arts & recreation | 2021 |
| Enhancing Excellence Ltd | Professional & scientific | 2023 |
| Enigma Electric Ltd | Construction | 2022 |
| Enjoy-A-Ball (UK) Limited | Arts & recreation | 2006 |
| ENS Tailwind Holdings Limited | Administrative & support | 2017 |
| Enterprise Construction (Scotland) Ltd | Construction | 2022 |
| Envy Hair (Scotland) Limited | Other services | 2010 |
| Epcc Holdings Limited | Financial services | 2021 |
| Epic Contracts Limited | Professional & scientific | 2023 |
| Epic Think Learn C.I.C. | Education | 2023 |
| Epicurus Limited | Real estate | 1994 |
| Epoint Limited | Manufacturing | 2020 |
| Equilibrium Financial Consultancy Limited | Financial services | 2003 |
| Equilibrium Travel Management Ltd | Administrative & support | 2011 |
| Equinox Access Solutions Ltd | Administrative & support | 2009 |
| Equinox Management Solutions Ltd | Administrative & support | 2016 |
| ERS Racing Limited | Wholesale & retail | 2005 |
| Essarrelle Limited | Transport & logistics | 2008 |
| Essport Autoparts Limited | Wholesale & retail | 2016 |
| Estantia Care Limited | Real estate | 2008 |
| Estantia Limited | Construction | 2002 |
| Estate Agents Scotland Ltd | Real estate | 2026 |
| Ethaniel Studios Ltd. | Information & communication | 2017 |
| Ethax Ltd | Information & communication | 2019 |
| Etlp Plus Ltd. | Professional & scientific | 2023 |
| Etru Solutions Limited | Information & communication | 2019 |
| Eurotech Marine Data Services Limited | Professional & scientific | 2007 |
| Evenheart Limited | Administrative & support | 2026 |
| Everest Inn DUN Limited | Hospitality | 2022 |
| Evergrow Properties Limited | Real estate | 2019 |
| EVI Renewables Ltd | Construction | 2017 |
| Evolution a M Limited | Financial services | 2012 |
| Evolve Plumbing & Heating Ltd | Construction | 2018 |
| Evolve Primary Care Consultants Limited | Health & social care | 2021 |
| EW Gannon Pipeline Services Limited | Administrative & support | 2009 |
| EWS Lettings Ltd | Real estate | 2025 |
| Excel Care PVT Ltd | Health & social care | 2019 |
| Excel Digital Solutions Ltd | Administrative & support | 1985 |
| Exchange Performance Consulting Limited | Professional & scientific | 2020 |
| Executive Delivery Service Ltd | Transport & logistics | 2014 |
| Express Garage Doors Limited | Construction | 2003 |
| External Cleaning Co (Scotland) Ltd | Administrative & support | 2023 |
| EYE to YOU Limited | Health & social care | 2014 |
| EZ Cabz Ltd | Transport & logistics | 2025 |
| EZ Taxis Ltd | Transport & logistics | 2025 |
| F&F Joinery Ltd | Construction | 2023 |
| F.A.M. Trading (Scotland) Ltd | Wholesale & retail | 2016 |
| F.J. Logan Ltd. | Financial services | 2008 |
| FAB Landscaping Ltd | Professional & scientific | 2018 |
| Fabrilis Scotland Limited | Construction | 2021 |
| Fabulous Finish Housekeeping Limited | Administrative & support | 2024 |
| Facial Surgery Scotland Limited | Health & social care | 2022 |
| Fairy Holdings Ltd | Education | 2026 |
| Falkland Developments Ltd | Construction | 2019 |
| Family First Enterprises Limited | Other services | 2024 |
| Fampro Real Estate Ltd | Real estate | 2022 |
| FAN2CANOPY Ltd | Administrative & support | 2021 |
| Faria Grocers Ltd | Extraterritorial | 2023 |
| Farid Hillend Engineering Limited | Manufacturing | 1973 |
| FAS UK Fire Group Ltd | Public administration | 2024 |
| Fastrax Motor Sports Limited | Arts & recreation | 1999 |
| Fawdon Ltd | Hospitality | 2026 |
| Fawn & Fable Ltd | Hospitality | 2025 |
| FD Maziarz Ltd | Transport & logistics | 2021 |
| FDS Systems & Service Ltd | Administrative & support | 2023 |
| Ferdous Limited | Hospitality | 2008 |
| Ferry Saunas Ltd | Health & social care | 2025 |
| Fezzil Limited | Administrative & support | 2019 |
| FFS International (UK) Ltd | Professional & scientific | 2024 |
| Ffuhrmann Limited | Professional & scientific | 2012 |
| Fhmw Trading Limited | Real estate | 2023 |
| Fibre-Uk Ltd | Information & communication | 2022 |
| Fife Airport Transfers Ltd | Transport & logistics | 2025 |
| Fife Business Consultants Ltd | Professional & scientific | 2016 |
| Fife Car Cosmetics Limited | Wholesale & retail | 2024 |
| Fife Caravan Services Ltd | Transport & logistics | 2024 |
| Fife Cleaning Services Ltd | Administrative & support | 2017 |
| Fife College Limited | Extraterritorial | 2007 |
| Fife College Prison Education Limited | Extraterritorial | 2016 |
| Fife Community Garden Network Ltd | Agriculture & fishing | 2023 |
| Fife Curry House Ltd | Hospitality | 2025 |
| Fife Decor and Maintenance Ltd | Construction | 2025 |
| Fife Energy Management Ltd | Other services | 2019 |