| Dunfermline Athletic Football Club Limited | Arts & recreation | 1921 |
| Dunfermline Auto Electrics Ltd | Wholesale & retail | 2020 |
| Dunfermline Autocentre Limited | Extraterritorial | 1985 |
| Dunfermline BS Nominees Limited | Administrative & support | 1988 |
| Dunfermline Business Services Ltd | Administrative & support | 2021 |
| Dunfermline Carnegie Hockey Club | Arts & recreation | 2023 |
| Dunfermline Central Mosque Ltd | Other services | 2018 |
| Dunfermline City Taxis Ltd | Transport & logistics | 2023 |
| Dunfermline CT Taxis Ltd | Transport & logistics | 2024 |
| Dunfermline Development Company Limited | Construction | 2022 |
| Dunfermline Dual Control Car Hire Ltd | Administrative & support | 2025 |
| Dunfermline Eng/Tech Solutions Limited | Professional & scientific | 2023 |
| Dunfermline Events CIC | Wholesale & retail | 2025 |
| Dunfermline Golf Club Limited | Arts & recreation | 2023 |
| Dunfermline Home Furnishings Ltd. | Wholesale & retail | 2004 |
| Dunfermline Islamic Centre Limited | Other services | 2017 |
| Dunfermline Motor Company Limited | Wholesale & retail | 2020 |
| Dunfermline Nails & Spa 42 Ltd | Other services | 2023 |
| Dunfermline Photography Ltd | Professional & scientific | 2023 |
| Dunfermline Physiotherapy Limited | Health & social care | 2021 |
| Dunfermline Podiatry Centre Ltd | Health & social care | 2015 |
| Dunfermline Shopfitting Ltd | Manufacturing | 2022 |
| Dunfermline Tennis and Bridge Club Limited | Arts & recreation | 1966 |
| Dunfermline VIP Turkish Barber Ltd | Other services | 2023 |
| Duninodi Limited | Professional & scientific | 2022 |
| Dunlop Oceanic Ltd | Manufacturing | 2024 |
| Dunsire Associates (Electrical) Limited | Construction | 1973 |
| Duocleaning Ltd | Administrative & support | 2021 |
| Duradiamond Software Limited | Manufacturing | 2012 |
| Duvi Estates Limited | Real estate | 2024 |
| DX Tech Services Limited | Administrative & support | 2016 |
| Dycore Ltd | Real estate | 2019 |
| Dynamics Coaching and Guiding Ltd | Professional & scientific | 2025 |
| DZZ Consultants Ltd | Administrative & support | 2013 |
| E Moffat Training (Scotland) Limited | Education | 2025 |
| E&A Turkish Barbers Ltd | Administrative & support | 2020 |
| E-Construction Ltd | Construction | 2014 |
| E.S. Fleet Support Limited | Manufacturing | 1993 |
| Earlscove Ltd | Professional & scientific | 2015 |
| Earn $Femi Ltd | Information & communication | 2018 |
| Earthbreath Ltd | Wholesale & retail | 2019 |
| Earthy Books Limited | Information & communication | 2017 |
| EAS Global Ltd | Wholesale & retail | 2024 |
| East Coast Detailer's Ltd | Wholesale & retail | 2024 |
| East Coast Tours Ltd | Administrative & support | 2025 |
| East Coast Trades Ltd | Construction | 2025 |
| East Neuk Foods Ltd | Hospitality | 2019 |
| Eastend Roofing and Property Maintenance Ltd | Construction | 2023 |
| Easter Clune Ltd | Agriculture & fishing | 2025 |
| Eastgate Office Centre Management Company Limited | Administrative & support | 2016 |
| Eastons Training Limited | Other services | 2015 |
| EB Estates Limited | Real estate | 2017 |
| Eccles Heating Services Limited | Construction | 2022 |
| Eclipse (IP) Ltd | Administrative & support | 2007 |
| Eco Clean Masterton Ltd | Administrative & support | 2025 |
| Eco Pedals Limited | Wholesale & retail | 2010 |
| Eco Ship Design Ltd | Professional & scientific | 2022 |
| Ecoalgaform PVT Ltd | Agriculture & fishing | 2024 |
| Ecobi Limited | Agriculture & fishing | 2020 |
| Ecolodgy International Ltd | Professional & scientific | 2023 |
| Ecsr Limited | Administrative & support | 2017 |
| Eden Healthcare Ltd. | Health & social care | 2005 |
| Edge Radio Ltd | Information & communication | 2021 |
| Edin Taxi Services Ltd | Transport & logistics | 2023 |
| Edinburgh Campervans Limited | Administrative & support | 2017 |
| Edinburgh City Window Cleaning Limited | Other services | 2014 |
| Edinburgh Global Limited | Professional & scientific | 2019 |
| Edinburgh Telemedicine Solutions Ltd. | Information & communication | 1998 |
| Edindale Limited | Hospitality | 2019 |
| Edinmech Electrical Limited | Construction | 2022 |
| Edrm Properties Limited | Real estate | 2018 |
| Edwards Bar Ltd | Hospitality | 2023 |
| Edwards Incorporated Limited | Hospitality | 2026 |
| EDZ Property Ltd | Real estate | 2025 |
| EE21 Limited | Professional & scientific | 2020 |
| EGP Employment Services Limited | Administrative & support | 2019 |
| EH1 Management Limited | Hospitality | 2018 |
| Eight Concepts Ltd | Hospitality | 2021 |
| Eighteen Degrees Limited | Information & communication | 2003 |
| EIR Solutions Ltd | Health & social care | 2025 |
| EIT Group Ltd | Administrative & support | 2015 |
| Ej&Z Ltd | Real estate | 2024 |
| EJH Trading Limited | Hospitality | 2021 |
| Ekko Waste Solutions Ltd | Water & waste | 2010 |
| EKO Hardware Trading Company Ltd | Wholesale & retail | 2024 |
| EL Polako Properties Limited | Real estate | 2025 |
| ELB Software Ltd | Information & communication | 2024 |
| Elderslie Energy Limited | Other services | 2014 |
| Electronics 168 Ltd | Manufacturing | 2012 |
| Elevate Property Stagers Limited | Professional & scientific | 2025 |
| Elgin Inns Limited | Hospitality | 2025 |
| Elgin Investments (Scotland ) Limited | Hospitality | 2020 |
| Elgin Properties (Scotland) Limited | Real estate | 2023 |
| Elite Bathroom Agency Limited | Wholesale & retail | 2015 |
| Elite Estate Solutions Fife Ltd | Real estate | 2025 |
| Elite Painters and Decorators Ltd | Construction | 2015 |
| Elite Stonemasonry Ltd | Construction | 2024 |
| Ella Electrical Ltd | Construction | 2016 |
| Ella Jackson Veterinary Services Ltd | Professional & scientific | 2025 |
| Elliot Caunce Photography Ltd | Information & communication | 2023 |