| Fife Engineering Company Limited | Manufacturing | 1958 |
| Fife Free Mortgages Ltd | Financial services | 2023 |
| Fife Golf Studio Ltd | Arts & recreation | 2021 |
| Fife HMO Rentals Ltd | Real estate | 2025 |
| Fife Holdings Limited | Real estate | 2020 |
| Fife Homes Ltd | Real estate | 2021 |
| Fife Housing Group Yourplace Limited | Administrative & support | 2010 |
| Fife Neurology Services Limited | Health & social care | 2024 |
| Fife Physiotherapy Centre Ltd | Health & social care | 2018 |
| Fife Podiatry Hub Ltd | Health & social care | 2026 |
| Fife Property Agency Ltd | Real estate | 2025 |
| Fife Quality Butchers Ltd | Wholesale & retail | 2025 |
| Fife School of Music Ltd | Arts & recreation | 2024 |
| Fife Sports & Leisure Trust Limited | Arts & recreation | 2008 |
| Fife Swim Centre Ltd | Arts & recreation | 2014 |
| Fife Travel Ltd | Transport & logistics | 2020 |
| Fife Vehicle Services Ltd | Wholesale & retail | 2023 |
| Fife Wide 2 MAN and Van Ltd | Transport & logistics | 2020 |
| Fifeside Recovery and Transport Limited | Transport & logistics | 2025 |
| FIG Tree Software Limited | Information & communication | 2019 |
| Fight Store UK Ltd | Wholesale & retail | 2023 |
| Fill the GAP Learning Limited | Education | 2018 |
| Finely Pressed Domestic Services Ltd | Administrative & support | 2018 |
| Finlan - Medical Affairs Consultancy Ltd | Professional & scientific | 2023 |
| Fire Station Creative Limited | Arts & recreation | 2013 |
| Fire Station Creative Trading Limited | Arts & recreation | 2014 |
| Firestalk Ltd | Information & communication | 2019 |
| First Foot Fife Ltd | Health & social care | 2019 |
| First Steps Energy (Scotland) Ltd | Other services | 2024 |
| Firstplace Properties Ltd | Real estate | 2016 |
| Firth GIN Ltd | Wholesale & retail | 2020 |
| Fiscal Futures Group Ltd | Professional & scientific | 2021 |
| Fish Express Ltd | Hospitality | 2025 |
| Fitball Limited | Education | 2025 |
| Five Star BNB Ltd | Real estate | 2017 |
| Flagdor Limited | Extraterritorial | 2021 |
| Flagstaff Bar Limited | Hospitality | 2021 |
| Flauless Beauty Ltd | Other services | 2015 |
| Flear & Thomson (Perth) Limited | Wholesale & retail | 2020 |
| Flear & Thomson (Stirling) Limited | Wholesale & retail | 2017 |
| Flexenergy Limited | Administrative & support | 2001 |
| Flexi Business Support Limited | Administrative & support | 2020 |
| Flow Design Architects Ltd | Professional & scientific | 2020 |
| Flowaqua Limited | Manufacturing | 2023 |
| Flowerburn Medical Limited | Health & social care | 2021 |
| Fluid EYE Productions Limited | Information & communication | 2006 |
| Focus Construction & Developments Scotland Limited | Construction | 2019 |
| Food Shack Fife CIC | Hospitality | 2023 |
| Food Tech Systems Ltd | Hospitality | 2016 |
| Football AID Trading Limited | Other services | 2000 |
| Football FUN Factory (Community 105) Limited | Arts & recreation | 2023 |
| Footballcompany Limited | Arts & recreation | 2013 |
| Foothorn Developments Limited | Real estate | 2017 |
| Foothorn Investments Limited | Real estate | 2017 |
| Footprint Media Production Ltd | Information & communication | 2022 |
| Forbes Medical Cannabis Scotland Limited | Health & social care | 2022 |
| Fords Food Wholesale Limited | Wholesale & retail | 2024 |
| Foreman AI Limited | Information & communication | 2026 |
| Forgan Trading Limited | Wholesale & retail | 2015 |
| Forged Filament Ltd | Wholesale & retail | 2024 |
| Forrester Park Leisure Limited | Arts & recreation | 1996 |
| Forth Aesthetics Limited | Health & social care | 2021 |
| Forth Asbestos Services Ltd | Professional & scientific | 2016 |
| Forth Brickwork (Scotland) Limited | Construction | 2011 |
| Forth Clean Ltd | Administrative & support | 2026 |
| Forth Construction Ltd | Construction | 2012 |
| Forth Crossing Security Ltd | Administrative & support | 2025 |
| Forth Dual Controls Ltd | Administrative & support | 2025 |
| Forth Events Ltd | Hospitality | 2022 |
| Forth Marine Textiles Limited | Manufacturing | 2003 |
| Forth Pipe Organs Limited | Manufacturing | 2002 |
| Forth Property Maintenance Limited | Real estate | 2024 |
| Forth Quant Ltd | Information & communication | 2024 |
| Forth Tech Holdings Limited | Financial services | 2024 |
| Forth Tech Limited | Information & communication | 2001 |
| Forth Travels Ltd | Transport & logistics | 2016 |
| Forthroots Limited | Construction | 2013 |
| Forthside Flavours Ltd | Manufacturing | 2025 |
| Fortune Group Limited | Hospitality | 2025 |
| Fortune Ventures Limited | Hospitality | 2021 |
| Forum on Nuclear Legacies CIC | Professional & scientific | 2025 |
| Fossgar Services Limited | Manufacturing | 2015 |
| Four Braes Ltd | Agriculture & fishing | 2022 |
| Fourfront Global Group Ltd | Wholesale & retail | 2026 |
| Foursight Enterprises Limited | Real estate | 1989 |
| FOX CUB Properties Limited | Households | 2026 |
| Fractional Works Limited | Information & communication | 2025 |
| Framwellgate Ltd | Hospitality | 2026 |
| Fraser Dunnett Heating Solutions Ltd | Professional & scientific | 2024 |
| Frederick Hugh Tait Ltd | Extraterritorial | 2024 |
| Friendly Finds Limited | Wholesale & retail | 2025 |
| Front Line Builders Merchants Limited | Wholesale & retail | 1989 |
| Frontshore Limited | Hospitality | 2026 |
| FTM Homes Ltd | Real estate | 2025 |
| FTM Oasis Ltd | Arts & recreation | 2022 |
| Fulton and Inglewood Limited | Wholesale & retail | 2016 |
| Fusion Ceramics Limited | Manufacturing | 2017 |
| Fusion Nexus Ltd | Information & communication | 2022 |
| Fusion Whisky Limited | Other services | 2015 |
| Future Finance Scotland Ltd | Financial services | 2021 |