| Enterport LP | | 2015 |
| Enterprise Community Centre CIC | Education | 2018 |
| Enterprise Investments (Dundee) Limited | Real estate | 2011 |
| Enterprise Mentoring (Property) Limited | Real estate | 2014 |
| Enterprise Mentoring C.I.C. | Professional & scientific | 2004 |
| Enterprise Screen Productions Limited | Information & communication | 2005 |
| Enterson Management L.P. | | 2015 |
| Entertrade L.P. | | 2015 |
| Entities Ltd | Wholesale & retail | 2025 |
| Entity Games UK Ltd | Information & communication | 2026 |
| Entrust People Limited | Administrative & support | 2010 |
| Entrust Resource Solutions Ltd | Administrative & support | 2012 |
| Enzo (Scotland) Limited | Hospitality | 2018 |
| Enzo Recordings Limited | Information & communication | 2008 |
| Enzo's AYR Limited | Hospitality | 2024 |
| Enzo's Cucina Italiana Limited | Hospitality | 2021 |
| EOC Productions Ltd | Professional & scientific | 2023 |
| EP Wealth Management Limited | Financial services | 2003 |
| EPC Planning and Project Controls Ltd | Professional & scientific | 2023 |
| Epic Developments Dundee Ltd | Construction | 2023 |
| Epic Drams Ltd | Wholesale & retail | 2019 |
| Epipole Ltd | Manufacturing | 2011 |
| Episoft Ltd | Information & communication | 2019 |
| EPM Offshore Ltd | Construction | 2023 |
| Epmh Limited | Wholesale & retail | 2001 |
| Eraze Computer Software Ltd | Information & communication | 2020 |
| Ergotex Transport L.P. | | 2016 |
| Eric Young Auto Electrician Limited | Manufacturing | 2019 |
| Ern-Dec Limited | Construction | 2007 |
| Eros Electrical Ltd | Construction | 2013 |
| Errol Engineering Ltd | Professional & scientific | 2022 |
| Erzz Ltd | Households | 2024 |
| Escape Community Hub CIC | Arts & recreation | 2024 |
| Escape Dundee Ltd | Arts & recreation | 2024 |
| Escapez Limited | Information & communication | 2020 |
| Eshaal Food Ltd | Hospitality | 2025 |
| ESJ Property Investments Ltd | Real estate | 2016 |
| ESK (Holdings) Limited | Real estate | 2013 |
| ESK Holdco 1 Limited | Financial services | 2025 |
| ESK Properties Limited | Real estate | 2021 |
| ESK Salmon Management Limited | Administrative & support | 2005 |
| ESM Accountancy Ltd | Professional & scientific | 2024 |
| Espc Dundee Holdings Ltd | Other services | 2024 |
| Espc Dundee Ltd | Arts & recreation | 2024 |
| Esposito Alliance LP | | 2017 |
| Espress OH! Ltd | Hospitality | 2012 |
| Espresstech Ltd | Agriculture & fishing | 2015 |
| Essential Contract Cleaning Scotland Limited | Administrative & support | 2023 |
| Estate and Succession Planning Limited | Professional & scientific | 2018 |
| Etaxi Dundee Ltd | Transport & logistics | 2020 |
| Ethicss Ltd | Professional & scientific | 2024 |
| Etive Construction Limited | Professional & scientific | 2013 |
| Etmaccess Ltd | Construction | 2025 |
| Euclid 2025 Ltd | Financial services | 2025 |
| Euclid Trading Limited | Arts & recreation | 2009 |
| Euroasian Traders Ltd | Wholesale & retail | 2026 |
| European Chemicals & Co | | 1993 |
| Europenet Experts L.P. | | 2015 |
| Eurostreet L.P. | | 2015 |
| Eurotrend Business L.P. | | 2014 |
| EV Homecharge Install Limited | Construction | 2020 |
| EV101 Ltd | Transport & logistics | 2024 |
| Evagreen Cleaning Ltd | Administrative & support | 2026 |
| Evagreen Professional Services Limited | Transport & logistics | 2013 |
| Evaluate HR Limited | Professional & scientific | 2021 |
| Evans Brewery Ltd | Hospitality | 2019 |
| Event Production Group Ltd | Administrative & support | 2017 |
| Events 105 Limited | Professional & scientific | 2016 |
| Everest R2G Limited | Hospitality | 2021 |
| Evergreen Business Consulting Limited | Administrative & support | 2014 |
| Evergreen Treecare Limited | Administrative & support | 2012 |
| Evergreen Warehousing Service Ltd | Transport & logistics | 2021 |
| Everlast Windows and Doors Ltd | Construction | 2021 |
| Everlux Partners L.P. | | 2015 |
| Evermark Trading Ltd | Wholesale & retail | 2026 |
| Evermore Wellbeing (Scotland) Ltd | Other services | 2022 |
| Eversley Property Ltd | Real estate | 2025 |
| Everview Trading Limited | Wholesale & retail | 2015 |
| Everwarm Doors and Windows Ltd | Construction | 2022 |
| Everydayfinds Ltd | Wholesale & retail | 2025 |
| Evolution Resources Limited | Administrative & support | 2018 |
| Evorra Padel Limited | Arts & recreation | 2025 |
| Evotek Holdings Limited | Professional & scientific | 2018 |
| Evotek Limited | Professional & scientific | 2004 |
| EW Health Ltd | Health & social care | 2025 |
| Ewan Fraser Contracts Ltd | Manufacturing | 2017 |
| Ewan Fraser Limited | Real estate | 2021 |
| EWI Motor Engineers Ltd | Wholesale & retail | 2025 |
| EWL Solutions Limited | Professional & scientific | 2001 |
| EWY Systems Ltd | Construction | 2026 |
| Examwell Group LP | | 2017 |
| Exclozuk Ltd | Wholesale & retail | 2023 |
| Executive Partners Network Ltd | Administrative & support | 2021 |
| Exford Transit L.P. | | 2015 |
| Exfront Inter L.P. | | 2015 |
| Exhale Vapour Lounge Tech Ltd | Wholesale & retail | 2018 |
| Exile Fitness Academy Limited | Arts & recreation | 2021 |
| Exinet Development LP | | 2017 |
| Exmile Universal L.P. | | 2016 |
| Exmoor Organization L.P. | | 2016 |