| Exoticclean Services Ltd | Administrative & support | 2025 |
| Exotron L.P. | | 2013 |
| Expark Partners L.P. | | 2015 |
| Expected Values Consulting Ltd | Professional & scientific | 2018 |
| Expert Clinics Scotland Limited | Health & social care | 2019 |
| Expert Co L.P. | | 2015 |
| Expert Sales Consultancy Ltd | Professional & scientific | 2020 |
| Experts in English Limited | Administrative & support | 2013 |
| Explorer Heat Limited | Energy | 2014 |
| Expo Inter Associates L.P. | | 2015 |
| Expobrand L.P. | | 2014 |
| Expocross L.P. | | 2015 |
| Expoway Networks L.P. | | 2016 |
| Express Heating & Plumbing Ltd | Construction | 2013 |
| Express Laundry Dundee Ltd | Other services | 2025 |
| Expressmax L.P. | | 2015 |
| Exscientia AI Limited | Professional & scientific | 2012 |
| Extech Electrical Services Limited | Construction | 2025 |
| External Transformations Access Solutions Ltd | Construction | 2023 |
| Extractionrc Ltd | Transport & logistics | 2026 |
| Extrade Nordic L.P. | | 2015 |
| Extragrade L.P. | | 2015 |
| Extraroad Impex LP | | 2017 |
| Extrasteel Products L.P. | | 2014 |
| Extrawest L.P. | | 2015 |
| Exwave Worldwide L.P. | | 2014 |
| Exwood Trade L.P. | | 2015 |
| Eyam Vaccines and Immunotherapeutics UK Ltd | Professional & scientific | 2022 |
| Ezaan Investment Limited | Wholesale & retail | 2019 |
| F X Simulation Operating Ltd | Manufacturing | 2008 |
| F&A Estates Limited | Real estate | 2025 |
| F&E Ross Designs Ltd | Administrative & support | 2013 |
| F&L Stays Ltd | Hospitality | 2024 |
| F&M Properties (Dundee) Ltd | Real estate | 2020 |
| F&M Property (Dundee) Ltd | Real estate | 2014 |
| F&S Production Vehicles Limited | Administrative & support | 2022 |
| F. Mclaughlin Holdings Limited | Financial services | 2025 |
| F.M.S.E. Macfarlane Limited | Health & social care | 2003 |
| F1 Training Services (UK) Limited | Health & social care | 2013 |
| FAB Aesthetics Scotland Limited | Health & social care | 2024 |
| FAB Electrical Solutions Ltd | Construction | 2023 |
| Fable Antiques Ltd | Wholesale & retail | 2025 |
| Fabrication Machinery Ltd. | Manufacturing | 2011 |
| Face-It by MMS Limited | Wholesale & retail | 2022 |
| Factora Impex L.P. | | 2015 |
| Fade Asylum Ltd | Other services | 2024 |
| Faff Digital Limited | Information & communication | 2025 |
| Fair City Munchkins Day Nursery Limited | Education | 2014 |
| Fair City Scaffolding Limited | Construction | 1983 |
| Fair Fares Cabs Ltd | Transport & logistics | 2020 |
| Fair Price Fintry Ltd | Wholesale & retail | 2018 |
| Fairfield Community Hub Limited | Arts & recreation | 2018 |
| Fairmuir Paving Ltd. | Other services | 2016 |
| Fairmuir Properties Ltd | Real estate | 2026 |
| Fairmuir Roofing Ltd | Construction | 2021 |
| Fairouz 48 Limited | Hospitality | 2026 |
| Fairway Escapes Ltd | Real estate | 2025 |
| Fairwind Property Ltd | Real estate | 2019 |
| Falcon Lashes Limited | Other services | 2024 |
| Falconz Ltd | Hospitality | 2024 |
| Falkirk Asset Managers Limited | Real estate | 2003 |
| Falkirk Traders Ltd | Hospitality | 2019 |
| Falkirk Whisky Storage Limited | Administrative & support | 2026 |
| FAM Hospitality & Events Ltd | Hospitality | 2018 |
| Famh Dundee Ltd | Real estate | 2025 |
| Family Bay Ltd | Real estate | 2026 |
| Family Friends Homecare Limited | Health & social care | 2017 |
| Famlam Ltd | Wholesale & retail | 2023 |
| Fanduel Limited | Information & communication | 2007 |
| Fank 45 Ltd | Transport & logistics | 2025 |
| Fareland Ltd | Hospitality | 2021 |
| Farington Consultancy Ltd | Information & communication | 2025 |
| Farington House Properties Ltd. | Real estate | 2002 |
| Farington Joinery Ltd | Construction | 2026 |
| Farlane Donald Ltd | Construction | 2025 |
| Farlo Well Services Ltd | Professional & scientific | 2018 |
| Farnell Estates Limited | Real estate | 2006 |
| FARO5 Ltd | Real estate | 2016 |
| Farooq & Gair Properties Ltd | Real estate | 2023 |
| Farooq Traders Limited | Wholesale & retail | 2014 |
| Farquhar Engineering Limited | Professional & scientific | 2012 |
| Farrell Access Technician Ltd | Construction | 2014 |
| Farwest Company L.P. | | 2015 |
| Fasque Castle Limited | Real estate | 2017 |
| Fasque House Estate Limited | Construction | 2019 |
| Fasque House Properties Ltd. | Professional & scientific | 2010 |
| Fast Cleaning Dundee Ltd | Administrative & support | 2024 |
| Fast Drive Impex LP | | 2017 |
| Fast Drop Store Ltd | Wholesale & retail | 2024 |
| Fast Way Accents LP | | 2017 |
| Fastway Sellers Ltd | Wholesale & retail | 2025 |
| Faulkner Instrument Engineering Limited | Professional & scientific | 2016 |
| Faux Fancy Records Limited | Arts & recreation | 2015 |
| Fawns Interactive Ltd | Information & communication | 2023 |
| Fazal Travel Services Ltd | Transport & logistics | 2026 |
| Fazano Estate LP | | 2017 |
| Fazi Taxis Ltd | Transport & logistics | 2025 |
| FBW Consulting Limited | Professional & scientific | 2010 |
| FC Fish Merchants Limited | Wholesale & retail | 2021 |
| FC Holdings Dundee Limited | Financial services | 2024 |