| EL Cabezon Ltd | Wholesale & retail | 2019 |
| EL Madinah Ltd | Wholesale & retail | 2022 |
| Elaand Ltd | Wholesale & retail | 2021 |
| Elan Electronics Limited | Manufacturing | 1995 |
| Elara Corp. LP | | 2015 |
| Elassar Dental Implant Services Ltd | Health & social care | 2019 |
| Elavate Aesthetics Ltd | Health & social care | 2026 |
| Elberg Networks L.P. | | 2015 |
| ELC Drilling Limited | Mining & quarrying | 2003 |
| Elders Consulting Engineers LLP | | 2005 |
| Elderson Services Limited | Financial services | 2023 |
| Electric Bikes Scotland Limited | Wholesale & retail | 2013 |
| Electro Guard Fire & Security Limited | Administrative & support | 2005 |
| Electrocabs Dundee Ltd | Transport & logistics | 2020 |
| Electrotec Services Limited | Construction | 2015 |
| Elegance Brow and Lashes Limited | Other services | 2011 |
| Elegant Assets L.P. | | 2015 |
| Elegant Evergreens Decor Limited | Other services | 2025 |
| Elegant Modanero Services Ltd | Administrative & support | 2025 |
| Elektrocall Limited | Construction | 2018 |
| Elemental Crystals Limited | Wholesale & retail | 2018 |
| Elemental Specialist Recruitment Limited | Administrative & support | 2017 |
| ELF Bar Ltd | Wholesale & retail | 2025 |
| Elfrasertradesservices Limited | Manufacturing | 2014 |
| Elgin Artificial Grass Limited | Construction | 2020 |
| Elgog Ltd | Other services | 2020 |
| ELI Properties Ltd | Real estate | 2017 |
| Elie Harbour Operations Limited | Arts & recreation | 2025 |
| Elie Harbour Trust Limited | Arts & recreation | 1975 |
| Elite Alloy Wheels and Tyres Ltd | Wholesale & retail | 2014 |
| Elite Edge Educators Ltd | Education | 2023 |
| Elite Electrical (Scotland) Ltd | Construction | 2021 |
| Elite Electrical Dundee Ltd | Construction | 2020 |
| Elite Global Healthcare (UK) Ltd | Financial services | 2023 |
| Elite Global Healthcare Ltd | Professional & scientific | 2021 |
| Elite Scaffold Ltd | Construction | 2024 |
| Elix Outsourcing Ltd | Administrative & support | 2023 |
| Elizabeth Humble Jewellery Ltd | Manufacturing | 2021 |
| Eljoy Ltd | Health & social care | 2022 |
| Ella's Cleaning Services Ltd | Administrative & support | 2018 |
| Ellarose Homeware Limited | Wholesale & retail | 2025 |
| Ellenberg Estate LP | | 2017 |
| Elliot Estates Limited | Real estate | 2007 |
| Ellis Investments Dundee Limited | Health & social care | 2012 |
| Ellite Security Ltd | Administrative & support | 2023 |
| ELM Gardeners Limited | Administrative & support | 2015 |
| Elmbank Builders Limited | Construction | 2025 |
| Elmslea Estate Ltd | Real estate | 2025 |
| Elmwood Electrical (Dundee) Limited | Construction | 2026 |
| Elmwood Electrical G Ltd | Professional & scientific | 2024 |
| Elmwood Joinery Limited | Manufacturing | 2000 |
| Elmwood Slaters Limited | Construction | 2017 |
| ELS Edin Ltd | Hospitality | 2020 |
| Elvie (Trading) Limited | Manufacturing | 2016 |
| Elvie Framing (Dundee) Ltd | Manufacturing | 2022 |
| Elvoice Limited | Information & communication | 2021 |
| Elyangroup Ltd | Professional & scientific | 2025 |
| Elysia Tavern Ltd | Hospitality | 2019 |
| Elysian Homes (Scotland) Ltd | Construction | 2020 |
| Emaan Fatima Ltd | Wholesale & retail | 2025 |
| Emac Planning & Development Limited | Professional & scientific | 2017 |
| Emac Planning, Development & Design Limited | Professional & scientific | 2017 |
| Emarin Tech Ltd | Transport & logistics | 2025 |
| EMC Hydrographic Services Ltd | Administrative & support | 2015 |
| EMD Wellness Ltd | Health & social care | 2026 |
| Emdee Enterprises Ltd | Construction | 2026 |
| Emerald Classics Ltd | Wholesale & retail | 2022 |
| Emerald Playtime Limited | Education | 2021 |
| Emerald Trading Group Ltd | Wholesale & retail | 2025 |
| Emergency Medicine Consulting Limited | Health & social care | 2023 |
| EMG Services Limited | Professional & scientific | 2017 |
| Emigrantas Media L.P. | | 2015 |
| Emily Tighe Ltd | Other services | 2019 |
| Emma & Joseph Properties Ltd | Financial services | 2024 |
| Emma ROY Ltd | Wholesale & retail | 2017 |
| Emmock Estates Limited | Real estate | 2019 |
| Emmock Park Ltd | Administrative & support | 2015 |
| Emotorz Ltd | Wholesale & retail | 2022 |
| Empire Beauty Clinic Ltd | Other services | 2018 |
| Empire Plastering LH Ltd | Construction | 2026 |
| Empire Properties (JT) Ltd | Real estate | 2023 |
| Empire State Coffee Dundee Limited | Hospitality | 2018 |
| EMPIRE33 Ltd | Information & communication | 2026 |
| Emray Engineering Services Ltd. | Manufacturing | 2017 |
| Enable One Ltd | Information & communication | 2023 |
| Enders Analysis Limited | Professional & scientific | 1996 |
| Energomax L.P. | | 2015 |
| Energy Dundee 4 U Ltd | Administrative & support | 2016 |
| Energy Recovery Systems Ltd | Water & waste | 2012 |
| Energy Technical Services Ltd | Professional & scientific | 2005 |
| Energy Trilemma Solutions Limited | Professional & scientific | 2024 |
| Enervative Limited | Mining & quarrying | 2007 |
| Engineering Spreadsheets Ltd. | Real estate | 2006 |
| Enhance (Dundee) Limited | Other services | 2025 |
| Enhanced Intelligence Ltd | Information & communication | 2019 |
| Enmascarada Entertainment, Ltd. | Arts & recreation | 2010 |
| Enough Entertainment Ltd | Manufacturing | 2021 |
| Enresol Limited | Professional & scientific | 2007 |
| Enterford Inter L.P. | | 2016 |
| Entermark Trade LP | | 2015 |