| Spire Construction (NW) Limited | Construction | 2007 |
| Sports Distribution Limited | Wholesale & retail | 2020 |
| Spray Guns Direct Limited | Administrative & support | 2006 |
| Springhill International Limited | Wholesale & retail | 1994 |
| Sprinta Limited | Manufacturing | 2001 |
| Squirecroft Ltd | Manufacturing | 2012 |
| Ssec Limited | Professional & scientific | 2005 |
| ST Business Consulting Limited | Professional & scientific | 2012 |
| ST Chads Road Supporting Housing Limited | Health & social care | 2004 |
| ST Georges Park Apartments Management Company Limited | Households | 2022 |
| ST. Wystan's School | Education | 1966 |
| St.Christophers(Glossop)Limited | Health & social care | 1958 |
| Stable Close (Apartments) Management Company Limited | Households | 2025 |
| Staceys Bakery Limited | Manufacturing | 2002 |
| Staffordshire Clays Limited | Professional & scientific | 1991 |
| Staley Limited | Professional & scientific | 2003 |
| Stanley Fearn Cycles Ltd | Wholesale & retail | 2003 |
| Stapleford Heights Residents Management Company Limited | Households | 2025 |
| Steele Homes Limited | Real estate | 2004 |
| Sterigenics UK Limited | Health & social care | 1991 |
| Steve's Taxis (Cheshire) Limited | Transport & logistics | 2005 |
| Stonefirst Limited | Wholesale & retail | 2000 |
| Strathfield Estates Limited | Real estate | 1993 |
| Streamline Property Development Ltd | Construction | 2003 |
| Streamline Quality Builders Ltd | Construction | 2004 |
| Streason Limited | Professional & scientific | 1962 |
| Street Crane Company Limited | Manufacturing | 1958 |
| Structural Waterproofing Systems Limited | Administrative & support | 2000 |
| Strutt Engineering Ltd | Manufacturing | 2003 |
| Stuart Smith (Derby) Limited | Professional & scientific | 2003 |
| Stubbins and Hope Limited | Other services | 2002 |
| Stubley Lane Autocentre Ltd | Wholesale & retail | 2020 |
| Suburban Village PUB Company Limited | Hospitality | 2017 |
| SUE Carson Saddles Limited | Manufacturing | 2005 |
| Suma (Sales) Limited | Water & waste | 1986 |
| Sun Chemical Limited | Manufacturing | 1991 |
| Sunningdale (Yorkshire) Limited | Hospitality | 1982 |
| Sureturn Engineering Limited | Professional & scientific | 2004 |
| Swadlincote Window Co. Ltd. | Manufacturing | 1998 |
| Swift Development NW Limited | Construction | 2006 |
| Synergy Logistics Limited | Information & communication | 1972 |
| T & J Scoggins Limited | Agriculture & fishing | 2007 |
| T & R Slack Limited | Agriculture & fishing | 2005 |
| T M Glass & Glazing Services Limited | Manufacturing | 2005 |
| T N Webster & SON Limited | Manufacturing | 2004 |
| T Peel Roofing Contractors Limited | Construction | 2000 |
| T.D.P. Textiles Limited | Manufacturing | 1982 |
| Tacitus Projects Limited | Education | 2003 |
| Tapton Products Limited | Professional & scientific | 1946 |
| TBS Group (Midlands) Ltd | Wholesale & retail | 2024 |
| Techtrics Limited | Professional & scientific | 2000 |
| Tectonic Electrical Limited | Construction | 2006 |
| TED Johnson Plant Services Limited | Administrative & support | 2000 |
| Tetherworth Engineering (Sheepbridge) Limited | | 1976 |
| Tetley Capital Limited | Financial services | 2024 |
| Texstyle Solutions Limited | Wholesale & retail | 2006 |
| TF Jackson Farms Limited | Hospitality | 2003 |
| Thai Pavilion Limited | Hospitality | 2003 |
| THE Ashford Family Investment Company Limited | Professional & scientific | 2024 |
| THE Beauty Mill Treatment Rooms Limited | Other services | 2019 |
| THE Bulls Head (Monyash) Limited | Extraterritorial | 2003 |
| THE Calico Bazaar Ltd | Wholesale & retail | 2022 |
| THE Chic Hair Studio Limited | Professional & scientific | 2006 |
| THE Convenience Company (Nottingham) Limited | Administrative & support | 2013 |
| THE Derby Storewatch Association | Other services | 1999 |
| THE Falcon Inn (Long Whatton) Ltd | Hospitality | 2024 |
| THE Financial Supermarket Limited | Financial services | 1993 |
| THE Fletcher Group Belper Ltd | Administrative & support | 2018 |
| THE Giltbrook Dyers & Cleaners (Properties) Limited | Real estate | 2011 |
| THE Identity Store Limited | Wholesale & retail | 2001 |
| THE LMS Carriage Association | Other services | 2002 |
| THE Mortgage Zone Limited | Financial services | 2000 |
| THE Portland Hotel Limited | Extraterritorial | 2003 |
| THE Print Factory (Chesterfield) Ltd | | 1996 |
| THE Rolls-Royce Heritage Trust | Arts & recreation | 2006 |
| THE Spire Tea & Coffee Co Limited | Professional & scientific | 2003 |
| THE Square Management Company (Buxton) Limited | Households | 1999 |
| THE Sundial Farm (Brailsford) Management Company Limited | Households | 2001 |
| THE Taxi Centre (Derbyshire) Limited | Transport & logistics | 2006 |
| THE Terrace (Chinley) Management Company Limited | Households | 2003 |
| THE Travelling Car Valeter (Derby) Limited | Administrative & support | 2004 |
| THE WEE Dram Limited | Wholesale & retail | 2004 |
| THE Wellness Rooms CD Limited | Health & social care | 2024 |
| THE Willows (Harwell) Management Company Limited | Households | 2016 |
| Theme Team Limited | Hospitality | 1997 |
| Thomas-Zadora Associates | | 1995 |
| Three Jays Limited | Wholesale & retail | 2001 |
| Tiger Plant Training Ltd | Education | 2024 |
| TIM Horne Limited | Construction | 2003 |
| TIM Stubbs Limited | Administrative & support | 2001 |
| Time Services (UK) Limited | Administrative & support | 2003 |
| Timeactive Limited | Arts & recreation | 2003 |
| Timeless Moments Ltd | Manufacturing | 2006 |
| Tire Monitor System Limited | Manufacturing | 1997 |
| TK & K Recreation Limited | Real estate | 2024 |
| TOM Keating and Associates Limited | Professional & scientific | 2002 |
| Topflight Property Development Limited | Professional & scientific | 2000 |
| Topline PLY and Racking Ltd | Wholesale & retail | 2025 |
| Townwalk Limited | Real estate | 2004 |
| Tracey E Sharkey Ltd | Education | 2003 |