| Trafalgar Marine Services Limited | Manufacturing | 2003 |
| Trailmarks Ltd | Information & communication | 2016 |
| Trained by FOX Limited | Arts & recreation | 2021 |
| Tramway Museum Services (R&C) Limited | Wholesale & retail | 2007 |
| Travall Limited | Manufacturing | 1999 |
| Trawlerfresh Fish Limited | Wholesale & retail | 2003 |
| Trekstock Cancer Support Ltd | Administrative & support | 2009 |
| Trekstock UK Limited | Wholesale & retail | 2009 |
| Trent Motor Traction Company Limited(The) | Transport & logistics | 1913 |
| Trinity HCS Consulting Limited | Professional & scientific | 2007 |
| Trinity Mews Management Company (Chesterfield) Limited | Real estate | 2002 |
| Triumph Motorcycles Group Limited | Professional & scientific | 1987 |
| Trovetred Limited | Wholesale & retail | 1985 |
| TRS Commercials Limited | Wholesale & retail | 2004 |
| TRT Limited | Manufacturing | 2000 |
| Tyre Specialists (Bakewell) Limited | Wholesale & retail | 2006 |
| U P Litigation Limited | Hospitality | 2000 |
| Ukcg Group Limited | Manufacturing | 2010 |
| Ukcg Limited | Manufacturing | 2010 |
| Ukmatrix Ltd | Professional & scientific | 2000 |
| Ukopa | Transport & logistics | 2000 |
| UKR Transmissions Limited | Wholesale & retail | 1988 |
| Ultramarine Investments Ltd | Real estate | 2004 |
| Underwood Tree Surgeons Limited | Agriculture & fishing | 2003 |
| Uniglaze Windows Limited | Construction | 1995 |
| Universal Paving & Building Specialists Limited | Construction | 2006 |
| University of Derby | Professional & scientific | 1995 |
| Unstone Garden Centre Ltd | Wholesale & retail | 2003 |
| Upanalp Limited | Professional & scientific | 2005 |
| Upland Systems Limited | Information & communication | 2002 |
| Upper Farm Enterprises Ltd. | Hospitality | 1999 |
| Usability Matters Ltd | Administrative & support | 2017 |
| V B Wellness Ltd | Arts & recreation | 2025 |
| Vaillant Group UK Limited | Administrative & support | 1934 |
| Vaillant Industrial UK Limited | Manufacturing | 1972 |
| Valkyrie Design Limited | Professional & scientific | 2005 |
| Vallaton Limited | Manufacturing | 2006 |
| Valogrime Limited | Real estate | 1982 |
| Valve and Pipeline Engineering Services Limited | Extraterritorial | 2020 |
| Vauxhall Repair Centre Ltd | Wholesale & retail | 2012 |
| VC Italian Hospitality Ltd | Hospitality | 2024 |
| Verobelle Healthcare Services Ltd | Health & social care | 2021 |
| Vibrock Limited | Professional & scientific | 1999 |
| Vine Estates Limited | Real estate | 2008 |
| W Ball & SON (Holdings) Limited | Manufacturing | 1996 |
| W Fearnehough Limited | Manufacturing | 1990 |
| W. Burgess and Co Ltd | Information & communication | 2002 |
| W.E.C. (Leeds) Ltd | Manufacturing | 1998 |
| Wakebridge Estates Limited | Professional & scientific | 2021 |
| Wakefield Driveways Ltd | Construction | 2003 |
| Walkers Design Limited | Professional & scientific | 2002 |
| Wallingford Reach Management Company Limited | Households | 2021 |
| Walton Heath Apartments Management Company Limited | Households | 2022 |
| Ward Electrical Engineers Limited | Professional & scientific | 2003 |
| Warringtons Coaches Limited | Transport & logistics | 2006 |
| Wastonson Limited | Construction | 2004 |
| Water Technical Limited | Professional & scientific | 2005 |
| Waterfall Estates Ltd | Construction | 2011 |
| Wayne Lawrence Electrical Contractors Limited | Construction | 2005 |
| Weatherwise Home Improvements Limited | Construction | 2003 |
| Wellglade Limited | Professional & scientific | 1986 |
| Wells Property Development Limited | Real estate | 2005 |
| West Derbyshire Holdings Limited | Real estate | 1981 |
| West Special Fasteners Limited | Manufacturing | 1999 |
| Westfield Planning Ltd | Professional & scientific | 2004 |
| Westville Contractors Limited | Construction | 2002 |
| Wheeldon Manor Management Company Limited | Real estate | 2002 |
| Whitehead Developments Limited | Financial services | 1993 |
| Whites Fine Jewellers Limited | Wholesale & retail | 2003 |
| Whittaker (Chesterfield) LLP | | 2007 |
| Whittaker Engineering Services Limited | Construction | 2006 |
| Whittaker Standard House Ltd | Construction | 2023 |
| Wigleys Shoes Limited | Wholesale & retail | 2006 |
| Wilbur Chase Residents Management Company Limited | Households | 2021 |
| William LEE Limited | Manufacturing | 1991 |
| William Lennon & Co. (Footwear Manufacturers) Limited | Manufacturing | 1964 |
| William Potts & Sons Limited | Manufacturing | 1906 |
| William West Distribution Limited | Transport & logistics | 2001 |
| Williamson Estates Ltd | Real estate | 2025 |
| Willowgrove Holdings Ltd | Professional & scientific | 2015 |
| Wincraft Limited | Wholesale & retail | 1975 |
| Windmill Lane Financial Services Limited | Professional & scientific | 2013 |
| Windows 2000 Limited | Administrative & support | 1993 |
| Wingfield Store Ltd | Wholesale & retail | 2018 |
| Wirksworth Regeneration and Development C.I.C. | Professional & scientific | 2003 |
| Wistaston (Church Lane) Management Company Limited | Households | 2017 |
| Woodward Plumbing & Heating Limited | Manufacturing | 1998 |
| Word of Limited | Professional & scientific | 1998 |
| Wordamber Limited | Real estate | 1996 |
| Wychstone Ltd. | Professional & scientific | 1995 |
| Wyvern Management (Plot 4000) Limited | Real estate | 2000 |
| X Sofas Limited | Wholesale & retail | 2003 |
| Xuanlee Limited | Wholesale & retail | 2026 |
| Your Business UK Limited | Professional & scientific | 2007 |
| Zaliant Limited | Information & communication | 2006 |
| Zeerok Ltd | Wholesale & retail | 2021 |
| Zest Property Development Limited | Real estate | 2007 |
| Zeus Contracts Limited | Information & communication | 1995 |