| Eulalie Limited | Real estate | 2019 |
| Evolution (Scotland) Ltd | Administrative & support | 2023 |
| Evolution Business Services Limited | Professional & scientific | 2015 |
| Exotic Vets Ltd | Professional & scientific | 2023 |
| Ezenggileer Ltd | Agriculture & fishing | 2013 |
| F X Simulation (Scotland) Ltd | Arts & recreation | 2001 |
| Fabspect Ltd | Professional & scientific | 2015 |
| Falcon Contracts Limited | Construction | 2017 |
| Falkland Convenience Store Limited | Wholesale & retail | 2014 |
| Falkland Futures Trust Limited | Other services | 2026 |
| Falkland Rare Malt Limited | Wholesale & retail | 2023 |
| Falkland Rural Enterprises Limited | Agriculture & fishing | 1954 |
| Falkland Stewardship Trust | Education | 2010 |
| Falkland Whisky Company Limited | Wholesale & retail | 2023 |
| Fast'N Fresh Limited | Hospitality | 2002 |
| Faval (Cupar) Limited | Wholesale & retail | 1978 |
| Fay&Grey Ltd | Professional & scientific | 2025 |
| Fayre Earth Ltd | Wholesale & retail | 2022 |
| FCH HDD Trenchless Solutions Limited | Construction | 2025 |
| FEB Ltd | Health & social care | 2021 |
| Fellowship Film Limited | Information & communication | 2017 |
| Ferrigan's Flats Ltd | Hospitality | 2025 |
| Fetch My Feed Limited | Wholesale & retail | 2022 |
| Fetch ‘N’ Friends Dog Walking Fife Ltd | Agriculture & fishing | 2024 |
| Fiadhaich Gàirdeachas Ltd | Manufacturing | 2024 |
| Fife Athletic Club | Arts & recreation | 2015 |
| Fife Crops Limited | Agriculture & fishing | 1995 |
| Fife Food Network | Other services | 2010 |
| Fife Fruits Limited | Agriculture & fishing | 2005 |
| Fife Holiday Homes Limited | Hospitality | 2020 |
| Fife ZOO Ltd | Hospitality | 2015 |
| Financial Advice & Support (FAS) Limited | Professional & scientific | 2016 |
| Fiona Wilson Interiors Limited | Professional & scientific | 2018 |
| Fisher & Donaldson (Cupar) Limited | Other services | 2020 |
| Fisher & Donaldson (ST. Andrews) Limited | Manufacturing | 1962 |
| Fisher & Donaldson Limited | Extraterritorial | 1957 |
| Fishers Services Limited | Other services | 1979 |
| Fishers Topco Limited | Other services | 2013 |
| Five Kingdoms Brewery Limited | Manufacturing | 2019 |
| Five Vision Ltd | Hospitality | 2019 |
| FIVE2ELEVEN Ltd | Professional & scientific | 2018 |
| Fliskmillan Farmers 2002 | | 2002 |
| Flower and Co. Holdings Ltd | Financial services | 2026 |
| Fluid Management Ltd | Information & communication | 2021 |
| Flumill Limited | Professional & scientific | 2010 |
| Focus Business Consultancy Limited | Professional & scientific | 2018 |
| Fone Box (Scot) Ltd | Wholesale & retail | 2021 |
| Forbes Macpherson LLP | | 2021 |
| Forres.A. Ltd | Real estate | 2026 |
| Forrester Properties Limited | Real estate | 2019 |
| Fort Springs Ltd | Hospitality | 2021 |
| Forth & TAY Marine Limited | Professional & scientific | 1997 |
| Forth Events and Training Ltd | Hospitality | 2025 |
| Forth Park Games Limited | Information & communication | 2025 |
| Found by Chance Ltd | Real estate | 2026 |
| Four Eyes Radiology Ltd | Health & social care | 2026 |
| Foxall Studio Ltd | Administrative & support | 2005 |
| Fraser Co Property Ltd | Real estate | 2024 |
| Fraxinus Advisory Limited | Professional & scientific | 2024 |
| Fsew Limited | Other services | 2002 |
| FT Stonemasonry Ltd | Construction | 2020 |
| Future Skills and Learning Initiative Limited | Education | 2026 |
| FVM Medical Services Ltd | Professional & scientific | 2022 |
| G Commercial Property Limited | Households | 2023 |
| G J Bruins Properties Ltd | Administrative & support | 2023 |
| G Paterson Architect Ltd | Professional & scientific | 2014 |
| G Winter Property Maintenance and Landscaping Ltd | Construction | 2014 |
| G&L Labour Services Limited | Construction | 2025 |
| G.S.L. Plumbing & Heating Ltd | Construction | 2018 |
| Gain Line Impact Ltd | Professional & scientific | 2025 |
| Galbraith Petrie Ltd. | Education | 2002 |
| Gallant & Whyte Limited | Construction | 2023 |
| Gallus Recruitment Ltd | Administrative & support | 2024 |
| Gavin Love Orthopaedics Ltd | Health & social care | 2021 |
| Geebar Joinery Limited | Manufacturing | 2022 |
| Geillis Holdings Limited | Real estate | 2024 |
| GEM Psychology Ltd | Professional & scientific | 2025 |
| Gemrock Holdings Ltd | Real estate | 2025 |
| Gemrock Plumbing & Heating Ltd | Construction | 2019 |
| GENER8SCOTLAND Ltd | Wholesale & retail | 2018 |
| Generation Barbers Ltd | Other services | 2017 |
| Geoglic Ltd | Administrative & support | 2026 |
| George Storrar (Farmers) Limited | Agriculture & fishing | 1960 |
| GH Power Solutions Ltd | Manufacturing | 2024 |
| GHC Projects Ltd | Construction | 2024 |
| Gialink Limited | Information & communication | 2006 |
| Gieling Industries Ltd | Financial services | 2024 |
| Gladney Consultants Limited | Professional & scientific | 2015 |
| Gladney Investments Limited | Real estate | 2025 |
| Glenacre Holdings Limited | Financial services | 2017 |
| Glentarkie Limited | Real estate | 2009 |
| GM Supplies & Distribution Ltd | Wholesale & retail | 2009 |
| Go Financial Services Group Ltd | Financial services | 2017 |
| Golden POT Cupar Ltd | Hospitality | 2023 |
| Gooddrop Supply Co. Limited | Manufacturing | 2016 |
| Gourley Construction Limited | Construction | 2005 |
| Gpms Media Ltd | Information & communication | 2022 |
| GR Enterprises Limited | Professional & scientific | 2025 |
| GR Hughes Property Management Limited | Households | 2019 |
| Graham & Sherret LLP | | 2014 |