| Grant Wotherspoon Builders Ltd | Construction | 2015 |
| Gray Fabrication Limited | Manufacturing | 1981 |
| Greenhead Homes Limited | Construction | 2018 |
| Greenheart Contracts Ltd | Construction | 2010 |
| Gregor Allan Agri Ltd | Administrative & support | 2019 |
| Griselda Hill Pottery Limited | Manufacturing | 1989 |
| Growers Garden Limited | Manufacturing | 2018 |
| Grybai Ltd | Health & social care | 2026 |
| Guntur Enterprises Ltd | Real estate | 2017 |
| Guyana Limited | Wholesale & retail | 2001 |
| GYE Garden Care Limited | Administrative & support | 2010 |
| H.L. Stewart of Struthers Limited | Agriculture & fishing | 1960 |
| Hair at the Wynd Limited | Other services | 2015 |
| Hallpad Ltd | Information & communication | 2007 |
| Halo's Automotive Services Ltd | Wholesale & retail | 2025 |
| Halo's Construction Ltd | Construction | 2025 |
| Halo's Equestrian Ltd | Agriculture & fishing | 2025 |
| Halo's Fencing & Decking Ltd | Construction | 2025 |
| Halo's Holdings Ltd | Financial services | 2025 |
| Halo's Scaffolding Ltd | Construction | 2025 |
| Hame and Hearth Ltd | Real estate | 2026 |
| Hamilton Designed Ltd | Arts & recreation | 2023 |
| Handy Van Services Ltd | Transport & logistics | 2021 |
| Hatch Spirits Limited | Professional & scientific | 2017 |
| Hawklaw Consulting Ltd | Information & communication | 2014 |
| Hawkswood Country Estate Limited | Hospitality | 2020 |
| Hawkswood Resort Development Limited | Hospitality | 2020 |
| Heather Vets Limited | Professional & scientific | 2018 |
| Helen Mckenzie Smith Ltd | Wholesale & retail | 2026 |
| Henderson Black & Co Trustees Limited | Professional & scientific | 2014 |
| Heritage Hospitality Consulting Ltd | Administrative & support | 2020 |
| Hidden Hebrides Ltd | Administrative & support | 2016 |
| High Access Development Ltd | Construction | 2013 |
| Hillpark Management Services Limited | Professional & scientific | 2018 |
| Hillrig Holdings Limited | Financial services | 2023 |
| Hilton Produce Ltd | Energy | 2023 |
| Hogganfield Limited Partnership | | 2019 |
| Hoggs of Fife (Holdings) Limited | Financial services | 2019 |
| Home of Golf Vietnam Ltd | Administrative & support | 2026 |
| Howe Beer Project Ltd | Manufacturing | 2024 |
| Howe of Fife Rugby Football Club | Arts & recreation | 2013 |
| Howe of Fife Trading Limited | Arts & recreation | 2013 |
| Htma Ltd | Administrative & support | 2017 |
| Hugo Macmanaway Ltd | Manufacturing | 2026 |
| Hygge Homestyle Ltd. | Hospitality | 2025 |
| I & D Hunter Marketing Ltd. | Real estate | 2015 |
| I AM Well Gutsy Ltd | Other services | 2025 |
| Iain M Brown LLP | | 2014 |
| Iconic Garden Rooms Ltd | Construction | 2021 |
| Independent Security Supplies (Glasgow) Ltd | Wholesale & retail | 2011 |
| Indermis UK Limited | Health & social care | 2024 |
| Indos Cupar Ltd | Hospitality | 2018 |
| Industrial Services Plumbing and Heating Limited | Construction | 2025 |
| Inglis House Properties Ltd | Real estate | 2025 |
| Ingliston Trust | Other services | 2017 |
| Inicio Limited | Professional & scientific | 2022 |
| Innespections Ltd | Other services | 2017 |
| Intend Business Development Ltd | Professional & scientific | 2011 |
| Interiors by Tracy Smith Limited | Professional & scientific | 2015 |
| Inverie Ltd | Construction | 2019 |
| Iskrb Consulting Ltd | Health & social care | 2022 |
| Italiandely Ltd | Hospitality | 2024 |
| IVY Properties (Scotland) Limited | Real estate | 2024 |
| J C Bell Limited | Agriculture & fishing | 2008 |
| J Crawford Farrier Ltd | Agriculture & fishing | 2022 |
| J Jenkins Developments Ltd | Construction | 2024 |
| J Todd Holdings Ltd | Real estate | 2025 |
| J Todd Plant Services Ltd | Manufacturing | 2020 |
| J Wallace Electrical Ltd | Construction | 2019 |
| J&S Automotive Limited | Wholesale & retail | 2023 |
| J-Mech Fife Ltd | Professional & scientific | 2021 |
| J.B. Dall Limited | Real estate | 1956 |
| J.Fraser Holdings Ltd | Financial services | 2024 |
| Jackie Stewart Consulting Limited | Information & communication | 2014 |
| Jacs Catering Ltd | Hospitality | 2018 |
| James Hair & Co LLP | | 2009 |
| James Hair Group Limited | Administrative & support | 2016 |
| James S. Robertson & SON Limited | Agriculture & fishing | 1959 |
| Jarvis Property Projects Ltd | Real estate | 2020 |
| JB Dental Limited | Health & social care | 2021 |
| Jbrg Holdings Ltd | Financial services | 2025 |
| JD Subsea Ltd | Professional & scientific | 2017 |
| Jeffrey a C Meek LLP | | 2015 |
| Jeffrey Meek Limited | Professional & scientific | 2015 |
| JH Mechanical Services Ltd | Construction | 2025 |
| JJ Pilling Ltd | Transport & logistics | 2023 |
| JM Fire Protection Limited | Professional & scientific | 2021 |
| JMC Agricultural Contracts Limited | Administrative & support | 2013 |
| JMW Trading Enterprises Limited | Wholesale & retail | 2020 |
| Jodani Ecosse Ltd | Real estate | 2020 |
| JOE Cool (Holdings) Limited | Financial services | 2023 |
| JOE Cool (Property) Limited | Real estate | 2023 |
| JOE Cool (UK) Limited | Manufacturing | 1983 |
| JOE Cool Trading Limited | Manufacturing | 2021 |
| John Barr Contracts Ltd | Manufacturing | 2023 |
| John Lang Transport (Balmullo) Limited | Transport & logistics | 1975 |
| JPM Business Consultancy Limited | Professional & scientific | 2025 |
| JSK Developments Ltd | Construction | 2013 |
| Jtfw Ltd | Administrative & support | 2023 |
| JTH Ltd | Transport & logistics | 2026 |