| Craig Swinton Electrical Limited | Construction | 2012 |
| Crawford & Sons Limited | Real estate | 2023 |
| Creating Eden Ltd | Administrative & support | 2016 |
| Crewbraveheart Limited | Construction | 2025 |
| Croft Consultancy Training & Development Limited | Professional & scientific | 1999 |
| Crombie Trancoso Investments Limited | Financial services | 2011 |
| CRR Services (Scotland) Limited | Mining & quarrying | 2011 |
| Cruive Ltd | Hospitality | 2022 |
| Cupar Analytics Ltd | Professional & scientific | 2021 |
| Cupar Business Centre Limited | Administrative & support | 2013 |
| Cupar Dental & Implant Centre Ltd | Health & social care | 2025 |
| Cupar Development Trust Limited | Other services | 2015 |
| Cupar Executive Travel Limited | Transport & logistics | 2022 |
| Cupar Foods Limited | Hospitality | 2025 |
| Cupar Granary Limited | Construction | 1992 |
| Cupar North Farming LLP | | 2014 |
| Cupar Online Security Ltd | Administrative & support | 2022 |
| Cupar Rice Bowl Ltd | Hospitality | 2026 |
| Cupar Takeaway Ltd | Hospitality | 2025 |
| Cupar Tree Services Ltd | Administrative & support | 2016 |
| Cysys Consulting Ltd | Professional & scientific | 2014 |
| D Christie Electrical Services Ltd | Construction | 2026 |
| D&Rose Properties Limited | Real estate | 2025 |
| D-Mac Tanks and Construction Limited | Construction | 2013 |
| Dairsie Autopoint Limited | Wholesale & retail | 2025 |
| Daniel Gardner Buy to Lets Ltd | Real estate | 2024 |
| Daniel Gardner Electrical Contracting Limited | Construction | 2009 |
| Daniel Gardner Holdings Ltd | Other services | 2023 |
| Daniel Gardner Properties Limited | Real estate | 2014 |
| Darksnow Limited | Information & communication | 2013 |
| Datajack Limited | Information & communication | 2018 |
| Datca Limited | Financial services | 2026 |
| David Alison Coaching Ltd | Education | 2019 |
| Davtec Limited | Real estate | 2014 |
| Dempsey Decorating Limited | Construction | 2024 |
| Denbrae Drilling Consultants Limited | Administrative & support | 2014 |
| Derek Rutherford (Holdings) Limited | Administrative & support | 1995 |
| Dessers 786 Cupar Ltd | Hospitality | 2022 |
| DG Renewables Ltd | Professional & scientific | 2024 |
| DHF Plumbing & Heating Limited | Construction | 2026 |
| DHT Technologies Ltd | Manufacturing | 2016 |
| Different Solutions Limited | Information & communication | 2001 |
| Diffingo UK Limited | Professional & scientific | 2008 |
| DJC Autoservices Limited | Manufacturing | 2025 |
| DL Driving Services Limited | Administrative & support | 2019 |
| DMP Statistical Solutions UK Limited | Professional & scientific | 2005 |
| Dock of the Bay Ltd | Wholesale & retail | 2022 |
| Dogadora Ltd | Wholesale & retail | 2020 |
| Doloi Tea Company Limited | Agriculture & fishing | 1884 |
| Donibristle Construction Limited | Construction | 2017 |
| Donnelly Technical Services Limited | Professional & scientific | 2017 |
| Dory Bistro Limited | Hospitality | 2022 |
| Douglas Cairns Photography Ltd | Professional & scientific | 2020 |
| Downfield Limited | Manufacturing | 2014 |
| DP Testing Consultancy Ltd. | Information & communication | 2013 |
| DR David Stephens Limited | Health & social care | 2014 |
| Drew Gibb 2014 Ltd | Real estate | 2025 |
| Drinks Innovation UK Ltd | Professional & scientific | 2016 |
| Droneguard Ltd | Administrative & support | 2026 |
| Drumcarro Farm Limited | Agriculture & fishing | 2024 |
| Dryburgh Contracts Limited | Construction | 2010 |
| Duncraig Joiners and Builders Limited | Construction | 2017 |
| Dundee Farming Company Limited | Agriculture & fishing | 1953 |
| Dunfermline Private Hire Ltd | Transport & logistics | 2025 |
| Dura House Guest Services Limited | Hospitality | 2025 |
| DXY Land Services Ltd | Administrative & support | 2025 |
| Eagle Brae Limited | Hospitality | 2017 |
| East Coast Electrical Ltd | Construction | 2025 |
| East Coast Propshafts & Hydraulic Hoses Ltd | Manufacturing | 2016 |
| East Neuk Developments Ltd | Construction | 2025 |
| EB House Limited | Real estate | 2015 |
| Eden Civils Ltd | Construction | 2019 |
| Eden Springs Fife Ltd | Arts & recreation | 2019 |
| Eden Valley Prawns Ltd | Agriculture & fishing | 2023 |
| Eden Veterinary Practice | Professional & scientific | 2016 |
| Eden Woodcraft Limited | Construction | 2017 |
| Eden-Dale Estate Agency Limited | Professional & scientific | 1972 |
| Edenside Agricultural & Commercial Holdings Limited | Real estate | 2009 |
| Edinburgh City Escape Limited | Hospitality | 2017 |
| Edinburgh Psych Limited | Health & social care | 2023 |
| Ejkw Ltd | Construction | 2025 |
| EL Resales Ltd | Wholesale & retail | 2025 |
| Elie Holiday Park Limited | Hospitality | 2020 |
| Elliott Homes & Plant Ltd | Construction | 2012 |
| Elliott Production Services Ltd | Construction | 2019 |
| Ellis Energy Consulting Ltd | Professional & scientific | 2019 |
| Elmwood Golf Ltd | Arts & recreation | 2024 |
| EMBERFLOW5 Ltd | Construction | 2024 |
| Employment Cultural and Community Trust Limited | Administrative & support | 1986 |
| Energyex Group Limited | Mining & quarrying | 2025 |
| Energyscape (UK) Limited | Professional & scientific | 2025 |
| Engeco Ltd | Construction | 2012 |
| Eplan Aviation Ltd | Manufacturing | 2019 |
| Eplansystems Ltd | Manufacturing | 2025 |
| Equi-Back Limited | Professional & scientific | 1998 |
| Equineox Ltd | Professional & scientific | 2024 |
| Erin Clara Limited | Administrative & support | 2008 |
| Estate Investment Portfolio Ltd | Households | 2023 |
| Etec Design Ltd | Information & communication | 2012 |
| Ettles (UK) Limited | Professional & scientific | 2012 |