| South Cheshire Glass Limited | Manufacturing | 2017 |
| South Cheshire Homes Ltd | Real estate | 2018 |
| South Cheshire Painters & Decorators Limited | Construction | 2010 |
| Sovereign Automotive Limited | Professional & scientific | 2009 |
| Sowlved Limited | Information & communication | 2024 |
| SP Engineering Services Ltd | Wholesale & retail | 2026 |
| SP Grogan and Sons Limited | Construction | 2021 |
| Space Intel Ltd | Manufacturing | 2025 |
| Sparco Electrical Services Limited | Construction | 2022 |
| Spark Distillery Ltd | Manufacturing | 2021 |
| Spark Farm Ltd | Real estate | 2021 |
| SPD 1 Limited | Real estate | 2008 |
| SPD Distribution Ltd | Extraterritorial | 2024 |
| Spec Accounting Services Limited | Professional & scientific | 2004 |
| Spec Tile Limited | Wholesale & retail | 2002 |
| Specialist Driver Ltd | Transport & logistics | 2016 |
| Spectrum GAS Systems Limited | Manufacturing | 1995 |
| Speed Remiza Ltd | Transport & logistics | 2026 |
| Spencer & Co Surveyors Limited | Professional & scientific | 2009 |
| Spencer Hustwick Surveyors Limited | Professional & scientific | 2008 |
| Spenko International Limited | Manufacturing | 2020 |
| SPG Security Systems (UK) Limited | Administrative & support | 2003 |
| Sphathisene Limited | Health & social care | 2021 |
| Spicedise Limited | Hospitality | 2026 |
| Spill the Beans Coffee Ltd | Hospitality | 2025 |
| Spinney Garage Limited | Wholesale & retail | 1977 |
| Spiritual Rockstar Ltd | Other services | 2022 |
| Splhv Limited | Professional & scientific | 2015 |
| SPM GAS Ltd | Construction | 2025 |
| Spoilt 4 Choice of Bredbury Limited | Hospitality | 2018 |
| Spoke Consulting & Marketing Ltd | Professional & scientific | 2023 |
| Spoon and Maiden Ltd | Wholesale & retail | 2019 |
| Sports Admin Services Limited | Administrative & support | 2012 |
| Spotlight AI Consulting Limited | Information & communication | 2024 |
| Sprig Property Ltd | Real estate | 2023 |
| SPS (Agricultural) Ltd | Manufacturing | 2015 |
| Spuds N Spice Ltd | Hospitality | 2026 |
| Spyder-Labs (Emea) Limited | Information & communication | 2014 |
| SQL Consultancy Ltd | Information & communication | 2016 |
| Square Lettings Developments Ltd | Construction | 2024 |
| Square Sun Ltd | Manufacturing | 2025 |
| Square Telecom Limited | Information & communication | 2018 |
| Squirrel Joinery Ltd | Construction | 2025 |
| SR Rail Hire Ltd | Transport & logistics | 2020 |
| SRG Response Medical Ltd | Health & social care | 2020 |
| Ssas Reviews Administration Limited | Financial services | 2020 |
| Ssas Reviews Limited | Financial services | 2014 |
| Ssay Limited | Wholesale & retail | 2024 |
| SSI Investments Ltd | Real estate | 2010 |
| ST Andrews Court (Crewe) Management Company Limited | Households | 2000 |
| ST Sarah Home Health Care Ltd | Health & social care | 2024 |
| ST. Matthews Court Management (Fenton) Limited | Real estate | 1998 |
| Stable Vehicle Contracts LLP | | 2009 |
| Staffordshire Electrical UK Limited | Construction | 2019 |
| Staffordshire Mini Specialist Ltd | Wholesale & retail | 2024 |
| Staffordshire Neurological OT Services Ltd | Health & social care | 2022 |
| Staffordshire Printing Limited | Manufacturing | 1994 |
| Staffordshire Waste Limited | Construction | 2016 |
| Staicud Ltd | Transport & logistics | 2026 |
| Stanchion Living Limited | Health & social care | 2015 |
| Standsure Training Solutions Limited | Professional & scientific | 2021 |
| Stanowsky Ltd | Transport & logistics | 2025 |
| Stanton Wealth Limited | Financial services | 2025 |
| Star Afrique Ltd | Wholesale & retail | 2024 |
| Star Glaze UK Limited | Other services | 2016 |
| Starbrite Windows (Whitchurch) Limited | Wholesale & retail | 1990 |
| STATE2 Security Ltd | Administrative & support | 2024 |
| Station Park (Crewe) Limited | Real estate | 2010 |
| Stationery Box (Retail) Limited | Professional & scientific | 2005 |
| Stationery Box Limited | Professional & scientific | 2007 |
| Steele Fencing Ltd | Administrative & support | 2025 |
| Steele Heating & Plumbing Limited | Construction | 2012 |
| Steele Properties Limited | Real estate | 2016 |
| Steele Renovations Limited | Construction | 2025 |
| Stelios V8 Limited | Transport & logistics | 2024 |
| Stella Photography Ltd | Professional & scientific | 2017 |
| Stellar Global Consortium Limited | Health & social care | 2019 |
| Stellar Real Estate Limited | Real estate | 2019 |
| Stellar Sales Consulting Ltd | Information & communication | 2025 |
| Stem IS the Answer Ltd | Education | 2025 |
| Step Legal Limited | Professional & scientific | 2018 |
| Step Wise Property Ltd | Real estate | 2026 |
| Stephen Matthews & Associates Holdings Limited | Financial services | 2019 |
| Stephen Matthews & Associates Limited | Professional & scientific | 2001 |
| Stereg Property Limited | Real estate | 2023 |
| Sterling Clinics Group Limited | Health & social care | 2015 |
| Sterling Pharmacy Limited | Wholesale & retail | 1995 |
| Steve Clark Training Limited | Other services | 2014 |
| Stevedotluis Limited | Wholesale & retail | 2021 |
| Stevedotluis Properties Limited | Hospitality | 2024 |
| Stici Family Ltd | Wholesale & retail | 2023 |
| Stiller Tankers Ltd | Extraterritorial | 2025 |
| STL Scaffolding Services Limited | Construction | 2014 |
| Stoke Hall Barns Ltd | Real estate | 2021 |
| Stoke Packaging Supplies Ltd | Manufacturing | 2011 |
| Stone Biostatistics Limited | Information & communication | 2016 |
| Stone Quantity Surveying Ltd | Professional & scientific | 2023 |
| Stonedeaf Productions Ltd. | Arts & recreation | 2018 |
| Stop & Go Service Centre Ltd | Wholesale & retail | 2026 |
| Stop Shop Crewe Ltd | Wholesale & retail | 2026 |