| Sattva Organic Products Limited | Wholesale & retail | 2019 |
| Saturn Capital Limited | Financial services | 2014 |
| Satya Advisors Ltd | Other services | 2023 |
| Savvy Choices Limited | Financial services | 2024 |
| Scalable - IT Ltd | Wholesale & retail | 2017 |
| Scandishire Property Group Ltd | Real estate | 2017 |
| Scarab Construction Ltd | Construction | 2014 |
| Science Boffin Labs Ltd | Wholesale & retail | 2024 |
| Scion Property Ltd | Real estate | 2021 |
| Scitus Services Ltd | Construction | 2013 |
| Scitus Servicing Ltd | Construction | 2022 |
| Scom Group Limited | Information & communication | 2019 |
| Scom Services Limited | Information & communication | 2021 |
| Scotford Insulations Limited | Construction | 2015 |
| Scott Cooper Limited | Wholesale & retail | 1945 |
| Scotts Hydraulics & Agri Services Limited | Manufacturing | 2010 |
| Sebastian James Kitchens Ltd | Manufacturing | 2025 |
| Secondsyt Limited | Information & communication | 2021 |
| Secret Cottage Limited | Hospitality | 2014 |
| Secret Feasts Cotswolds Limited | Hospitality | 2025 |
| Secret Gardens (Cotswold) Ltd | Administrative & support | 2018 |
| Security Development Associates Limited | Professional & scientific | 2010 |
| SEE Electrics Ltd | Manufacturing | 2010 |
| Seed Product Design Ltd | Professional & scientific | 2025 |
| Select Enclosures Ltd | Extraterritorial | 2019 |
| Selexa Group Limited | Administrative & support | 2020 |
| Selexa Life Sciences Limited | Extraterritorial | 2025 |
| Selwych Limited | Water & waste | 1994 |
| Sensical Ltd | Manufacturing | 2025 |
| Sentinel Systems Limited | Manufacturing | 2005 |
| SERIES60 Limited | Information & communication | 2014 |
| Serity Limited | Information & communication | 2014 |
| Sertain Consulting Ltd | Professional & scientific | 2024 |
| Seven Counties Media Photography Ltd | Professional & scientific | 2019 |
| Seventeen Things Ltd | Wholesale & retail | 2025 |
| Severells Fields Community Interest Company | Agriculture & fishing | 2025 |
| Sewell Mullings Logie LLP | | 2010 |
| SGD Accountancy Ltd | Professional & scientific | 2018 |
| Shamiso Ltd | Professional & scientific | 2020 |
| Shannon Harris Coaching Limited | Other services | 2023 |
| Sharvell Property Ltd | Real estate | 2017 |
| Sheeghra UK Limited | Transport & logistics | 2021 |
| Sheep Street PUB Co Ltd | Hospitality | 2024 |
| Sheep&Chic Limited | Wholesale & retail | 2017 |
| Shefford Park Shoot Ltd | Arts & recreation | 2013 |
| Shelburne Farming LLP | | 2011 |
| Shell's Clever Company Limited | Information & communication | 2013 |
| Sheltered Housing Rentals Limited | Real estate | 2021 |
| Shepherd Marine Limited | Wholesale & retail | 2004 |
| Sheppard Enterprises Ltd | Real estate | 2021 |
| Sherston Wine Company Limited | Wholesale & retail | 1975 |
| Sherwood & Sons Ltd | Administrative & support | 2006 |
| Shine Master Cleaning Limited | Administrative & support | 2025 |
| Shoeworks Limited | Wholesale & retail | 2009 |
| Shrinking Space CIC | Arts & recreation | 2015 |
| Shropshire Shavings Company Ltd | Wholesale & retail | 2026 |
| Shustoke Barn Limited | Hospitality | 2012 |
| Sidley Auto Service Ltd | Wholesale & retail | 2024 |
| Sierra Victor IT Ltd | Information & communication | 2024 |
| Sigma Principal Capital Limited | Financial services | 2016 |
| Silberhorn Advisory Services Ltd | Professional & scientific | 2022 |
| Silbury Coins Limited | Other services | 2010 |
| Siluette Care Ltd | Health & social care | 2023 |
| Silverbill Properties Limited | Real estate | 2013 |
| Silverpoint Studios Limited | Arts & recreation | 2010 |
| Simmons Clark Ltd | Professional & scientific | 2014 |
| Simon Edward Associates Limited | Financial services | 2023 |
| Simpler Advice Limited | Real estate | 2008 |
| Simply Crystals Limited | Wholesale & retail | 2011 |
| Simply Opex Limited | Public administration | 2022 |
| Simply Plans Limited | Administrative & support | 2007 |
| Simply Properties (Cotswolds) Limited | Real estate | 2023 |
| Simply Scaffolding Limited | Construction | 2020 |
| Simplytics Ltd | Information & communication | 2024 |
| Singlepoint Networks Limited | Professional & scientific | 1984 |
| Sirensound Digital UK Limited | Information & communication | 2001 |
| Sirius Organics Limited | Agriculture & fishing | 2020 |
| Site Access Location Attendance Systems Limited | Information & communication | 2019 |
| Site Hire Ltd | Wholesale & retail | 2016 |
| Sitech Surveying Services Limited | Construction | 1996 |
| Situs Services Ltd | Extraterritorial | 2022 |
| Sizzle & BOO Ltd | Professional & scientific | 2022 |
| SJ Taylor Consulting Ltd | Professional & scientific | 2019 |
| SK FD Services Ltd | Professional & scientific | 2018 |
| Skako Concrete Limited | Manufacturing | 2024 |
| Skomer Limited | Manufacturing | 1993 |
| Skyhub Pro Ltd | Information & communication | 2026 |
| Skylark Air Services Ltd | Transport & logistics | 2013 |
| Slow Love Knitwear Limited | Wholesale & retail | 2022 |
| Smart DFT Solutions Limited | Other services | 2023 |
| Smart Rtms Limited | Health & social care | 2015 |
| Smart Study LLP | | 2021 |
| Smartaccess Systems Limited | Professional & scientific | 2024 |
| Smash N Grill Ltd | Hospitality | 2026 |
| Smesurance Limited | Financial services | 2021 |
| Smilewright Ltd | Health & social care | 2020 |
| SML Law Limited | Extraterritorial | 2016 |
| Snooty Flamingo Ltd | Hospitality | 2025 |
| SO Fresh & SO Clean UK Ltd | Administrative & support | 2016 |
| Soap Opera (Cirencester) Limited | Administrative & support | 2025 |