| Resonate Therapy Limited | Administrative & support | 2021 |
| Resource and Waste Solutions Partners Ltd | Water & waste | 2020 |
| Rest and Root Ltd | Wholesale & retail | 2025 |
| Restorate (Holdings) Ltd | Wholesale & retail | 2021 |
| Restpaper Holdings Limited | Financial services | 2025 |
| Restpaper Limited | Financial services | 1988 |
| Retreat & Rewild Ltd | Arts & recreation | 2024 |
| Reuseus Limited | Health & social care | 2020 |
| Reverse Vending Ltd | Extraterritorial | 2011 |
| RFS Holdco 1 Limited | Financial services | 2018 |
| RFT Design Consultants Ltd | Professional & scientific | 2023 |
| Riarlo Property Ltd | Real estate | 2026 |
| Richard Hale Ltd | Professional & scientific | 2014 |
| Richlee Consulting Limited | Professional & scientific | 2023 |
| Ride 24/7 Ltd. | Wholesale & retail | 2006 |
| Ridgeback Partners Ltd | Professional & scientific | 2015 |
| Ridgeway Structures Ltd | Manufacturing | 2018 |
| Rigi Woodworks Limited | Manufacturing | 2023 |
| Riley & Jojo Limited | Wholesale & retail | 2025 |
| Ripp Sporting Limited | Administrative & support | 2020 |
| Risk Assured Limited | Administrative & support | 2009 |
| Risk Literate Ltd | Professional & scientific | 2019 |
| Rivington Property Holdings Limited | Households | 2013 |
| RJT Cirencester Ltd | Wholesale & retail | 2022 |
| RM24 Limited | Information & communication | 2016 |
| RMT Estates Ltd | Real estate | 2025 |
| RNS Motors Limited | Wholesale & retail | 2002 |
| ROB FOX Landscape Contractors Ltd | Administrative & support | 2019 |
| ROB Walker Broadcasting Limited | Information & communication | 2008 |
| Roben Homes Limited | Construction | 2005 |
| Robert Dickson Consulting Ltd | Professional & scientific | 2010 |
| Roch 38 Limited | Professional & scientific | 2010 |
| Rock and Roll Stew Music Ltd | Information & communication | 2006 |
| Rock the Cotswolds Ltd | Professional & scientific | 2014 |
| Rockwealth Cotswolds Ltd | Financial services | 2025 |
| Rocky Clinton Limited | Arts & recreation | 2009 |
| Roll ME UP Ltd | Manufacturing | 2022 |
| Roman Saltway Limited | Administrative & support | 2026 |
| Rookery Software Limited | Information & communication | 2011 |
| Room 14 Limited | Administrative & support | 2011 |
| Rooms With Intention Ltd | Wholesale & retail | 2025 |
| Roots Admin Ltd | Information & communication | 2023 |
| Rosa Q Ltd | Wholesale & retail | 2015 |
| Rostant Limited | Arts & recreation | 2010 |
| ROW Ergx Ltd | Arts & recreation | 2025 |
| Rowan Dartington Holdings Limited | Financial services | 2010 |
| Roxon Electrical Limited | Construction | 2007 |
| Royal Agricultural College Limited | Extraterritorial | 2013 |
| Royal Agricultural University | Education | 1908 |
| Royal Agricultural University Enterprises Limited | Wholesale & retail | 1992 |
| Royal Agricultural University Student Union | Education | 2013 |
| RP Social Business Consulting Ltd | Professional & scientific | 2013 |
| RPE Investments Limited | Administrative & support | 2015 |
| Rsschaf Ltd | Administrative & support | 2018 |
| Rtfm Consultants Limited | Information & communication | 2007 |
| Ruffnready Ltd | Manufacturing | 2014 |
| Rupert Beck Consultant Urological Services Ltd | Health & social care | 2012 |
| Rural Pursuits UK Ltd | Arts & recreation | 2023 |
| RVM Systems Limited | Water & waste | 2017 |
| RWB Event Hire Ltd | Hospitality | 2018 |
| RWZ Clinical Service Limited | Professional & scientific | 2007 |
| S & D Fire Door Sets Limited | Manufacturing | 2026 |
| S C Plant Ltd | Construction | 2014 |
| S E J Productions Ltd | Wholesale & retail | 2026 |
| S E Property Division Ltd | Real estate | 2021 |
| S J Charles Property Management Ltd | Real estate | 2022 |
| S Squared Mechanical Services Limited | Construction | 2020 |
| S Turner Electrical Ltd | Manufacturing | 2009 |
| S&J Building Works Ltd | Construction | 2024 |
| S.J.Phillips & Sons(Kemble)Limited | Agriculture & fishing | 1969 |
| Sacred Group Ltd | Wholesale & retail | 2020 |
| Safedocs Ltd | Professional & scientific | 2023 |
| Safemach Inspections Ltd | Extraterritorial | 2022 |
| Safemach Safety Ltd. | Professional & scientific | 2020 |
| Saga Aerospace Limited | Professional & scientific | 2020 |
| Sage Electrical Solutions Ltd | Construction | 2021 |
| Salah Catering Ltd | Hospitality | 2023 |
| Salon 7 (Cirencester) Limited | Other services | 2015 |
| SALPAUL6 Ltd | Real estate | 2020 |
| Salsal Incorporated Ltd | Real estate | 2023 |
| Salt Way Publishing Limited | Information & communication | 2004 |
| Salter.Co.Uk Limited | Wholesale & retail | 2000 |
| Saltway Global Limited | Information & communication | 2004 |
| Saltway Properties Limited | Real estate | 2015 |
| Saltwells Holdings Limited | Extraterritorial | 2021 |
| Saltwells Management Limited | Real estate | 2021 |
| Salubrium Limited | Professional & scientific | 2013 |
| SAM Evans Architecture LLP | | 2020 |
| SAM Wilczak Design Ltd | Arts & recreation | 2005 |
| Samdaha Limited | Professional & scientific | 2026 |
| Samuel Miles Limited | Real estate | 2008 |
| Samuel Miles Wootton Bassett LLP | | 2017 |
| Sandford Business Solutions Limited | Administrative & support | 2019 |
| Sandy 2014 Limited | Financial services | 2014 |
| Santlo Limited | Professional & scientific | 2009 |
| Sarah Gallop Counselling Limited | Administrative & support | 2025 |
| Sarah Ruggins Ltd | Health & social care | 2025 |
| Sarah Tingey Sonography Limited | Health & social care | 2012 |
| SAS Build Limited | Construction | 2015 |
| SAS Developments (Swindon) Limited | Real estate | 2015 |