| Startset Limited | Real estate | 1986 |
| Stash Self Storage Ltd | Transport & logistics | 2002 |
| Statham Properties Ltd | Construction | 2023 |
| Statham Properties Platform Limited | Real estate | 2024 |
| Statham Properties Short Stay Solutions Ltd | Real estate | 2025 |
| Statutory Safety Inspections Limited | Professional & scientific | 2004 |
| Staveley Car Wash Ltd | Other services | 2021 |
| Staveley and Wyles Holdings Limited | Professional & scientific | 2012 |
| Staveley Automats Limited | | 1964 |
| Staveley Barber Ltd | Other services | 2025 |
| Staveley Guest Inn Limited | Hospitality | 2024 |
| Staveley Hall Garden Nursery Ltd | Wholesale & retail | 2008 |
| Staveley Mobility Ltd | Wholesale & retail | 2024 |
| Stavros Holdings Limited | Financial services | 2020 |
| STB (Chesterfield) Ltd | Hospitality | 2012 |
| Steane Solar Limited | Construction | 2026 |
| Steel City Classics Limited | Wholesale & retail | 2017 |
| Steel Industries Limited | Extraterritorial | 2007 |
| Steel Rehab Ltd | Health & social care | 2022 |
| Steel Services (Chesterfield) Limited | Construction | 2011 |
| Stella & Co Hair Ltd | Other services | 2025 |
| Stellar Eleven Ltd | Real estate | 2017 |
| Stennack Residents Management Company Limited | Hospitality | 1999 |
| Stephanie Winter Foot Care Ltd | Health & social care | 2025 |
| Stephen Morgan Communication Support Limited | Professional & scientific | 2025 |
| Stephenson Plant Ltd | Administrative & support | 2018 |
| Sterling Construction & Building Ltd | Construction | 2012 |
| Sterling Machining Limited | Manufacturing | 2001 |
| Sterling Pipework Ltd. | Construction | 2003 |
| Sterling Staycations Limited | Real estate | 2025 |
| Sterry Farm Limited | Households | 2016 |
| Steve Byard Ltd | Agriculture & fishing | 2010 |
| Steve Diggers Limited | Mining & quarrying | 2015 |
| Steve Housden Business Services Ltd | Professional & scientific | 2005 |
| Steven Akers Limited | Other services | 2015 |
| Stevenage Property Developments Limited | Construction | 2007 |
| Stevenson Construction Ltd | Administrative & support | 2015 |
| Stewart Network Services Limited | Construction | 2025 |
| Stiperstones Ltd | Financial services | 2023 |
| Stirland Building Ltd | Construction | 2022 |
| Stirling Electrical Limited | Construction | 2006 |
| Stirling EVC Ltd | Construction | 2021 |
| Stixis Ltd | Arts & recreation | 2020 |
| STL Management Solutions Ltd | Professional & scientific | 2016 |
| Stockport Skills Academy Limited | Extraterritorial | 2020 |
| Stockton Construction Limited | Construction | 1999 |
| Stokkex (UK) Limited | Wholesale & retail | 2025 |
| Stokkt Limited | Wholesale & retail | 2024 |
| Stone & Slate Limited | Mining & quarrying | 2001 |
| Stone and Brickwork Contractors Ltd | Construction | 2016 |
| Stone Assets Limited | Real estate | 2022 |
| Stone Lodge Matlock Ltd | Hospitality | 2021 |
| Stonebridge Walk Management Company Limited | Households | 2026 |
| Stonecraft Developments Limited | Construction | 2015 |
| Stonegravel Supermarket Ltd | Wholesale & retail | 2019 |
| Stonelow Conservation and Construction Limited | Construction | 2017 |
| Stonemere Partnerships Limited | Construction | 2018 |
| Stonewell Living Ltd | Real estate | 2026 |
| Storage & Display Equipment Limited | Construction | 1992 |
| Storage 365 Limited | Administrative & support | 2024 |
| Storage Chesterfield Limited | Transport & logistics | 2020 |
| Storforth Lane Garage Limited | Wholesale & retail | 2008 |
| Storforth Lane Storage Ltd | Transport & logistics | 2017 |
| Storm Tableware Limited | Wholesale & retail | 2000 |
| Storm Water Consulting Ltd | Professional & scientific | 2026 |
| Stormshroud Solutions Limited | Administrative & support | 2012 |
| Storrs Electrical Ltd | Construction | 2016 |
| Storrs Property Ltd | Real estate | 2023 |
| Strada Rail Limited | Wholesale & retail | 2003 |
| Strada Real Estates Ltd | Real estate | 2020 |
| Straightcurves Creative Ltd | Education | 2015 |
| Straightfix Property Maintenance Limited | Construction | 2022 |
| Strain Solutions Limited | Professional & scientific | 2004 |
| Strange-Twig Theatre Company Ltd | Administrative & support | 2025 |
| Stratadenizen Ltd | Information & communication | 2025 |
| Strateclink Limited | Information & communication | 2022 |
| Strategic Care Consulting Limited | Professional & scientific | 2025 |
| Strategic Land Estates Limited | Construction | 2017 |
| Strategic Social Work Ltd | Health & social care | 2022 |
| Stratosphere Consultants Ltd | Hospitality | 2021 |
| Straygoat Writing Services Ltd | Administrative & support | 2012 |
| Street Structures Limited | Manufacturing | 2004 |
| Streetvision Ltd | Information & communication | 2025 |
| Streetwise Surveyors Ltd | Other services | 2014 |
| Stretford Valeting Limited | Wholesale & retail | 2018 |
| Stretton Manor Barn Ltd | Extraterritorial | 2019 |
| Stretton MOT Centre Ltd | Wholesale & retail | 2015 |
| Stretton Service Station Limited | Wholesale & retail | 2000 |
| Strictly Tyres Limited | Wholesale & retail | 2013 |
| Stringastory Ltd | Information & communication | 2017 |
| Strobe Precision Ltd. | Manufacturing | 2006 |
| Strong Hospitality Ltd | Hospitality | 2025 |
| Stuart Bradley Limited | Wholesale & retail | 1998 |
| Stuart Domestics Ltd | Other services | 2011 |
| Stuart Yates Decor Ltd | Construction | 2013 |
| Stubbs Motor Engineers Limited | Wholesale & retail | 2026 |
| Stubbs Property Ltd | Real estate | 2018 |
| Studio 12 Pilates Ltd | Arts & recreation | 2025 |
| Studio 9 Chesterfield Ltd | Other services | 2024 |
| Studio Aesthetics and PMU Ltd | Other services | 2024 |