| Spire Topco Limited | Financial services | 2025 |
| Spire Translations Limited | Professional & scientific | 2022 |
| Spire Vets Ltd | Professional & scientific | 2010 |
| Spire View (Chesterfield) Ltd | Hospitality | 2020 |
| Spire Wedding Fayres and Events Ltd | Administrative & support | 2024 |
| Spire Wholesale Flooring Ltd | Wholesale & retail | 2014 |
| Spirebirds Ltd | Wholesale & retail | 2022 |
| Spirestaffs Ltd | Agriculture & fishing | 2025 |
| Spirex Properties Ltd | Real estate | 2025 |
| Spirits High Events Limited | Arts & recreation | 2016 |
| Spirits of the Afterlife Limited | Administrative & support | 2020 |
| Spital Developments Limited | Construction | 1937 |
| Spital Services Limited | Extraterritorial | 2019 |
| SPJ Capital Ltd | Real estate | 2017 |
| Splat Products Limited | Manufacturing | 2014 |
| SPM 1A Limited | Financial services | 2023 |
| Spolding & Sons Ltd | Manufacturing | 2015 |
| Spoolhaven Limited | Households | 1984 |
| Sport Scholarship USA Ltd | Administrative & support | 2023 |
| Sportingold Auctions Limited | Wholesale & retail | 2025 |
| Sportsman Leisure Limited | Hospitality | 2018 |
| Spotty Dog Properties Ltd | Real estate | 2016 |
| Spraytec Plastering and Rendering Systems Ltd | Construction | 2012 |
| Spring Healthcare Support Services Limited | Health & social care | 1999 |
| Springbank Properties Ltd | Real estate | 2019 |
| Springhill Beauty Barn Limited | Other services | 2021 |
| Springs BFD Ltd | Hospitality | 2023 |
| Springwater Cars Limited | Wholesale & retail | 2014 |
| Springwell Properties (Chesterfield) Limited | Real estate | 2024 |
| Sprint Sports & Leisure Limited | Wholesale & retail | 1977 |
| Spud Heaven Ltd | Hospitality | 2025 |
| Spuds 4 U (Chester) Limited | Wholesale & retail | 2024 |
| Spuds 4 U (Macclesfield) Limited | Wholesale & retail | 2026 |
| SPUDS4U (Northwich) Limited | Wholesale & retail | 2026 |
| SQ1 DNB Ltd | Arts & recreation | 2023 |
| Squad JIU Jitsu Ltd | Arts & recreation | 2019 |
| Square One Property Consultants Limited | Professional & scientific | 2014 |
| Squareco Limited | Wholesale & retail | 2021 |
| Squizzer Limited | Information & communication | 2019 |
| Sram Service UK Limited | Manufacturing | 2023 |
| SRB Medical Consulting Limited | Health & social care | 2010 |
| Srh-Ec Ltd | Construction | 2015 |
| Srisiddi Limited | Professional & scientific | 2023 |
| SRS Rail System International Hire Ltd | Administrative & support | 2010 |
| SRS Rail System International Limited | Construction | 2010 |
| SSI Property Portfolio Ltd | Real estate | 2025 |
| Sspool Ltd | Manufacturing | 2025 |
| SSS Logistics and Transportation Limited | Transport & logistics | 2025 |
| SSV Foods Private Limited | Hospitality | 2025 |
| ST Bernadette's Centre CIC | Wholesale & retail | 2013 |
| ST Civils Ltd | Construction | 2022 |
| ST Fibre Solutions Limited | Other services | 2016 |
| ST Fried Chicken Ltd | Hospitality | 2021 |
| ST Helens Inn Ltd | Hospitality | 2026 |
| ST Joseph's Close Maintenance Limited | Real estate | 2019 |
| ST Lawrence Pre-School (North Wingfield) | Education | 2007 |
| ST Mary's Catholic High School Academy Trust | Education | 2012 |
| ST Mary's Walk (Wickford) Management Company Limited | Households | 2011 |
| ST Minver Management Limited | Households | 2021 |
| ST. Luke's Maintenance (Chesterfield) Limited | Other services | 1974 |
| ST. Mary's Ridge Estate Management Company Limited | Households | 2004 |
| ST. Mary’S Walk Newbold Management Company Limited | Households | 2019 |
| ST. Peter and ST. Paul School Trust(The) | Education | 1984 |
| Stablecaps Ltd | Information & communication | 2023 |
| Stables Property Services Ltd | Construction | 2021 |
| Stacked and Packed Limited | Wholesale & retail | 2026 |
| Stainless Steel Drums Limited | Administrative & support | 2018 |
| Stakesby Holdings Limited | Financial services | 2025 |
| Stal Limited | Manufacturing | 2003 |
| Stampco UK Ltd | Wholesale & retail | 2023 |
| Stan Civils Holdings Ltd | Construction | 2025 |
| Stan Civils Limited | Construction | 2021 |
| Stancliffe Homes (Bentley) Ltd | Construction | 2021 |
| Stancliffe Homes (Casterton) Ltd | Construction | 2026 |
| Stancliffe Homes (Forest) Ltd | Construction | 2018 |
| Stancliffe Homes (Gateford) Ltd | Construction | 2025 |
| Stancliffe Homes (Hackney) Ltd | Construction | 2026 |
| Stancliffe Homes (Oxcroft) Ltd | Construction | 2022 |
| Stancliffe Homes (Standford) Ltd | Construction | 2025 |
| Stancliffe Homes (Sutcliffe) Ltd | Construction | 2025 |
| Stancliffe Homes (Willow) Ltd | Construction | 2026 |
| Stancliffe Homes Limited | Construction | 2018 |
| Standards for Europe Limited | Professional & scientific | 2006 |
| Standing Tall Support | Health & social care | 2021 |
| Stanedge Golf Holdings Limited | Professional & scientific | 2019 |
| Stanedge Golf Limited | Arts & recreation | 2016 |
| Stanfree Farm Leisure Ltd | Hospitality | 2025 |
| Stanfree Farm Shop Limited | Wholesale & retail | 2021 |
| Stanfree Holdings Limited | Wholesale & retail | 2022 |
| Stanley Express Ltd | Transport & logistics | 2011 |
| Stanway Consulting Limited | Transport & logistics | 2012 |
| Stanwood Engineering Limited | Manufacturing | 1975 |
| Star Chesterfield Ltd | Hospitality | 2019 |
| Star EMB Limited | Manufacturing | 2006 |
| Star Park Galaxy Limited | Arts & recreation | 2025 |
| Starbridge Sports Limited | Administrative & support | 2012 |
| Starling BUS & Coaches Ltd | Manufacturing | 2018 |
| Starmann Plumbing Ltd | Construction | 2026 |
| Start Financial Planning Limited | Financial services | 2016 |
| Startkind Limited | Information & communication | 2025 |