| Sorby Village (Waverley 3A) Management Company Limited | Households | 2022 |
| Sorento Investment Holdings Limited | Real estate | 2023 |
| Sorrell's Groundworks Ltd | Water & waste | 2023 |
| Sorrels Cocoa Bakery Ltd | Manufacturing | 2021 |
| Soul Center Healing Hypnosis Ltd | Health & social care | 2023 |
| Sourmouthuk Ltd | Wholesale & retail | 2023 |
| South East Regional Assessment Centre Limited | Other services | 2015 |
| South Leverton Permaculture Limited | Agriculture & fishing | 2021 |
| South West Racking Limited | Wholesale & retail | 2021 |
| South Yorkshire Gymnastics Academy CIC | Education | 2026 |
| South Yorkshire Skills Academy Limited | Extraterritorial | 2020 |
| Southern Tea Merchants Limited | Extraterritorial | 2015 |
| Southgate Trading Limited | Wholesale & retail | 2001 |
| Sowter Homes Limited | Real estate | 2022 |
| SOX UK Official Ltd | Wholesale & retail | 2023 |
| Soxcraft Limited | Administrative & support | 2021 |
| SOZ 23 Ltd | Hospitality | 2025 |
| SP Accountancy Ltd | Professional & scientific | 2022 |
| SP Electrical Engineering and Design Limited | Professional & scientific | 2025 |
| SP1 Limited | Education | 2008 |
| Spa Engineering & Groundcare Machinery Services Ltd | Professional & scientific | 2003 |
| Spa Revive Ltd | Wholesale & retail | 2020 |
| Space and I Ltd | Professional & scientific | 2023 |
| Space Counselling Chesterfield Ltd | Health & social care | 2003 |
| Space Mustard Ltd | Administrative & support | 2024 |
| Spacecraft Garden Design Ltd | Professional & scientific | 2017 |
| Spacies Plumbing Ltd | Construction | 2025 |
| Span Engineering Ltd | Construction | 2012 |
| Spares Direct 2 U Limited | Wholesale & retail | 2011 |
| Sparkfix Ltd | Construction | 2024 |
| Sparky INK Studio Ltd | Information & communication | 2016 |
| Sparky Studio Ltd | Information & communication | 2024 |
| Sparrowhawk Capital LLP | | 2020 |
| Sparta Weld Ltd | Manufacturing | 2025 |
| Spartan Gardening Ltd | Administrative & support | 2019 |
| Sparton Group Property Holdings UK Ltd | Financial services | 2025 |
| Special Education Services Ltd | Education | 2018 |
| Special Educational Needs Support Advice and Assessment (Sensaaa) Ltd | Education | 2025 |
| Special Ingredients Limited | Hospitality | 2013 |
| Specialist 1ST Limited | Financial services | 2023 |
| Specific Purpose Limited | Real estate | 2008 |
| Spectrum Commercials Ltd | Wholesale & retail | 2014 |
| Spectrum Freight Limited | Transport & logistics | 1988 |
| Spectrum Packaging Solutions Ltd | Administrative & support | 2018 |
| Spectrum Welding Supplies (Holdings) Limited | Financial services | 2024 |
| Spectrum Welding Supplies Limited | Manufacturing | 1983 |
| Sped Limited | Wholesale & retail | 2024 |
| Speeddraw Limited | Real estate | 1994 |
| Speetley Equestrian Centre Ltd | Arts & recreation | 2012 |
| Speetley Healthcare Ltd | Extraterritorial | 2010 |
| Spell of Sparkle Limited | Administrative & support | 2026 |
| Spencer Allen Ltd | Professional & scientific | 2023 |
| Spencer Scaffolding Ltd | Construction | 2015 |
| Spharmex Ltd | Professional & scientific | 2011 |
| Spice of Asia Chesterfield Ltd | Hospitality | 2026 |
| Spice of Orient 666 Limited | Hospitality | 2023 |
| Spice Route Global Ltd | Wholesale & retail | 2025 |
| Spider Investors Limited | Extraterritorial | 2020 |
| Spirac Physiotherapy Limited | Health & social care | 2003 |
| Spiralway Limited | Transport & logistics | 1987 |
| Spire Access Solutions Ltd | Construction | 2019 |
| Spire Automotive Group Ltd | Wholesale & retail | 2025 |
| Spire Bars Ltd | Hospitality | 2025 |
| Spire Bellotte Consulting Limited | Information & communication | 2025 |
| Spire Bidco Limited | Financial services | 2025 |
| Spire Blinds Limited | Construction | 2022 |
| Spire Building Services (Chesterfield) Limited | Construction | 2010 |
| Spire Engineering Services Limited | Professional & scientific | 2021 |
| Spire Estates Limited | Real estate | 2002 |
| Spire Funeral Services Limited | Other services | 2016 |
| Spire Glazing Ltd | Construction | 2021 |
| Spire Golf Solutions Limited | Construction | 2012 |
| Spire Graphics Limited | Manufacturing | 1978 |
| Spire Holdings Ltd | Real estate | 2025 |
| Spire Homes Ltd | Construction | 2002 |
| Spire Hosting Limited | Information & communication | 2020 |
| Spire Hydraulics Limited | Manufacturing | 1984 |
| Spire Lawn Care Limited | Administrative & support | 2020 |
| Spire Lettings Ltd | Real estate | 2012 |
| Spire Machine Knives Ltd | Manufacturing | 2022 |
| Spire Machine Tools Limited | Professional & scientific | 2020 |
| Spire Midco Limited | Financial services | 2025 |
| Spire Model Distribution Limited | Wholesale & retail | 2007 |
| Spire Plumbing Limited | Other services | 2006 |
| Spire Property Services Ltd | Construction | 2025 |
| Spire Property Solutions Limited | Households | 2022 |
| Spire PUB Co Limited | Hospitality | 2022 |
| Spire Rail Solutions Limited | Extraterritorial | 2021 |
| Spire Recruitment Ltd | Administrative & support | 2014 |
| Spire Recruitment Solutions Limited | Administrative & support | 2013 |
| Spire School of Motoring Ltd | Education | 2023 |
| Spire Services (Chesterfield) Limited | Financial services | 2011 |
| Spire Sheff Limited | Wholesale & retail | 2024 |
| Spire Solar Limited | Construction | 2025 |
| Spire Sport Solutions Limited | Administrative & support | 2010 |
| Spire Steel Structures Ltd | Construction | 2025 |
| Spire Stone Mill Limited | Wholesale & retail | 2024 |
| Spire Storage Ltd | Transport & logistics | 2025 |
| Spire Structural Detailing Limited | Construction | 2016 |
| Spire Taverns Ltd | Hospitality | 2025 |