| Scribble and Splat Limited | Arts & recreation | 2025 |
| Scribe Furniture Ltd | Construction | 2010 |
| SD Events Catering Ltd | Hospitality | 2025 |
| SD Wilkinson Ltd | Professional & scientific | 2023 |
| SDC Security Development Consultants Limited | Professional & scientific | 2009 |
| SDE Electrical Limited | Construction | 2021 |
| SDE Energy Solutions Limited | Construction | 2022 |
| SDE Roofing Limited | Construction | 2023 |
| SDK Aesthetics & Beauty Limited | Other services | 2025 |
| SDM Catering Ltd | Hospitality | 2018 |
| Sdpd Assets Ltd | Administrative & support | 2022 |
| Sdpd Holdings Ltd | Financial services | 2022 |
| Sdpd Land Ltd | Real estate | 2022 |
| SDS Enterprises Ltd | Professional & scientific | 2024 |
| SDS Metalwork Ltd. | Construction | 2010 |
| Seadam Ltd | Wholesale & retail | 2024 |
| Seafresh Desalinators Limited | Manufacturing | 1997 |
| Seal Team Live Limited | Administrative & support | 2021 |
| Seal Team Systems Limited | Manufacturing | 2005 |
| Seals & Shelters Limited | Extraterritorial | 1986 |
| Search Selection Recruitment Ltd | Extraterritorial | 2009 |
| Seat Covers UK Ltd | Manufacturing | 2018 |
| Seatherny Outdoor Living Ltd | Wholesale & retail | 2023 |
| Seclinic Limited | Other services | 2025 |
| Second Chance Uniform Ltd | Other services | 2024 |
| Secondary Glazing Specialists Ltd | Construction | 2024 |
| Secure Chain Technology Group Ltd | Information & communication | 2019 |
| Securecase Limited | Information & communication | 2026 |
| Securicore Global Limited | Professional & scientific | 2023 |
| Securit (GB) Limited | Manufacturing | 2004 |
| Security Design (Leeds) Ltd | Administrative & support | 2012 |
| Securnet Solutions Ltd | Information & communication | 2022 |
| Sedgwick & Sons Limited | Wholesale & retail | 2007 |
| Seehow Designs Limited | Professional & scientific | 1996 |
| Selasecure Ltd | Construction | 2025 |
| Select Driveways & Paving Ltd | Administrative & support | 2025 |
| Select Industrial Supplies Ltd | Wholesale & retail | 2017 |
| Select International Products Limited | Wholesale & retail | 1995 |
| Select Properties (Chesterfield) Limited | Real estate | 2014 |
| Select Property (North) Limited | Real estate | 2017 |
| Self Assessment and Corporate Tax Limited | Professional & scientific | 1996 |
| Self Solutions (UK) Ltd | Information & communication | 2018 |
| Self Storage Yard Limited | Other services | 2012 |
| Selim Dental Limited | Health & social care | 2025 |
| Sellsold Ltd | Wholesale & retail | 2026 |
| Selwood & Sons Ltd | Transport & logistics | 2023 |
| Sema Fish Ltd | Hospitality | 2020 |
| Senate Training Limited | Administrative & support | 2000 |
| Send Health Consultancy Ltd | Health & social care | 2023 |
| Senescence Care Agency Ltd | Health & social care | 2016 |
| Senior Environmental Monitoring Limited | Manufacturing | 2016 |
| Senior Machinery Ltd | Wholesale & retail | 2012 |
| Sense Developments Limited | Construction | 1996 |
| Sentinel Medical Solutions Ltd | Professional & scientific | 2025 |
| Sentura Chauffeurs Ltd | Transport & logistics | 2023 |
| Sequence.Cool Ltd | Administrative & support | 2026 |
| Serasto Limited | Professional & scientific | 2017 |
| Sereco Windows Limited | Construction | 2022 |
| Seren Holdings (UK) Ltd | Administrative & support | 2011 |
| Serendipity Healthcare Ltd | Health & social care | 2007 |
| Serenity and Wellness Pilates Ltd | Other services | 2025 |
| Serenity Outdoor Living Ltd | Wholesale & retail | 2023 |
| Serenity Outsourced Solutions Ltd | Financial services | 2019 |
| Servo Cabling Services Ltd | Other services | 2016 |
| Seven Promotions Limited | Administrative & support | 2006 |
| Sevens Bar Ltd | Hospitality | 2019 |
| SGA Minis Ltd | Education | 2021 |
| SGB Properties Limited | Professional & scientific | 2005 |
| SGC Digital Limited | Professional & scientific | 2025 |
| SH Chesterfield Limited | Wholesale & retail | 2012 |
| SH Housing and Property Development Ltd | Wholesale & retail | 2023 |
| SH Plant (Chesterfield) Limited | Mining & quarrying | 2008 |
| Sh&Dw Property Ltd | Real estate | 2025 |
| Shahram Barbers Ltd | Other services | 2023 |
| Shakespeare Wood Ltd | Real estate | 2023 |
| Shambadek Draughting Services Limited | Professional & scientific | 2017 |
| Sharks SKI Club C.I.C. | Arts & recreation | 2012 |
| Sharma Consultancy Limited | Professional & scientific | 2017 |
| Sharon's Painting and Decorting Ltd | Construction | 2025 |
| SHC Properties (Midlands) Ltd | Real estate | 2021 |
| Shcl AVN Newco Spinout 8 Limited | Professional & scientific | 2015 |
| SHE Advisory Services Ltd | Professional & scientific | 2024 |
| She's Unstoppable Ltd | Other services | 2023 |
| Shed Grounds Maintenance Ltd | Administrative & support | 2003 |
| Sheepish Plaice Properties Limited | Real estate | 2022 |
| Sheff-Vend Limited | Wholesale & retail | 2025 |
| Sheffield Digital Academy Limited | Extraterritorial | 2020 |
| Sheffield Kitchen Centre Ltd | Construction | 2014 |
| Sheffield MMA Ltd | Education | 2022 |
| Sheffield Motorcycle Centre Limited | Wholesale & retail | 2006 |
| Sheffield Piston Rings Ltd | Extraterritorial | 2011 |
| Sheffield Scooters Limited | Wholesale & retail | 2002 |
| Sheffield Skills Academy Limited | Extraterritorial | 2019 |
| Sheffield Weightlifting Club CIC | Arts & recreation | 2025 |
| Sheglobal LLP | | 2016 |
| Shelagh Hayes Limited | Financial services | 2019 |
| Shepcote Engineering Limited | Manufacturing | 1988 |
| Shepherds Tree Rentals Limited | Real estate | 2021 |
| Sherides Collective Ltd | Hospitality | 2025 |
| Sherwood Court (Bolsover) Management Company Limited | Households | 2019 |