| Abbey Glass Chester Limited | Administrative & support | 2000 |
| Abbey Rentals Management Company Limited | Households | 2021 |
| Abbey School Limited | Health & social care | 2011 |
| Abbey Square Limited | Manufacturing | 2012 |
| Abbey Window Cleaning Limited | Administrative & support | 2015 |
| Abbeyfield Chester Society Limited (the) | Health & social care | 1963 |
| Abbeyfields Flat Management Company Limited | Households | 2002 |
| Abbiss Estates Ltd | Real estate | 2024 |
| Abbiss Property Services Ltd | Real estate | 2024 |
| Abbotpost Limited | Professional & scientific | 1996 |
| Abbots Mead Limited | Construction | 2003 |
| Abbots Mead Solutions Limited | Construction | 2008 |
| Abbots Park Dental Care Ltd | Health & social care | 2024 |
| Abbots Park Developments Ltd | Construction | 2025 |
| Abbots Park Wealth Management Ltd | Financial services | 2015 |
| Abbotts Building & Paving Limited | Construction | 2007 |
| ABC Cake Creations Limited | Hospitality | 2013 |
| ABC Cakes Ltd | Manufacturing | 2021 |
| ABC Lifestyle Ltd | Wholesale & retail | 2020 |
| ABC MK Limited | Financial services | 2016 |
| ABC Taxis Chester Ltd | Transport & logistics | 2021 |
| ABE Ashby Road Ltd | Extraterritorial | 2017 |
| ABE Construction Services Ltd | Construction | 2022 |
| ABE Investment & Management Services Ltd | Real estate | 2003 |
| Aben Properties Limited | Real estate | 2006 |
| Abersarn Ltd | Construction | 2022 |
| Aberteg Ltd | Professional & scientific | 2022 |
| Abhirati Plants and Pots Ltd | Agriculture & fishing | 2025 |
| Abigail Owen Architects Limited | Professional & scientific | 2020 |
| Ability Homes Limited | Real estate | 2007 |
| Ability Kitchens Ltd. | Wholesale & retail | 1999 |
| Abiza (UK) Ltd | Wholesale & retail | 2011 |
| Abodebookings Ltd | Information & communication | 2025 |
| Abrams Hughes Associates Limited | Professional & scientific | 2015 |
| ABS 88 Limited | Hospitality | 2025 |
| ABS IT Ltd | Professional & scientific | 2019 |
| Absolute Chiro Health Limited | Health & social care | 2023 |
| Absr Osula Limited | Education | 2024 |
| Abstract Hair Design Limited | Other services | 2017 |
| Abstract Nails and Beauty Ltd | Other services | 2020 |
| Abundance Accommodation Ltd | Real estate | 2026 |
| Abundant Properties Ltd | Construction | 2024 |
| AC Davlen Limited | Administrative & support | 2006 |
| AC Kave Ltd | Administrative & support | 2016 |
| Acacia of Chester Ltd | Wholesale & retail | 2013 |
| Academize Limited | Information & communication | 2024 |
| ACB Inspire Limited | Education | 2024 |
| Accelerate Change Limited | Professional & scientific | 2012 |
| Acceptable LLP | | 2013 |
| Access Digital Systems Limited | Professional & scientific | 2005 |
| Accession Network Ltd | Other services | 2024 |
| Accounts Direct (Chester) Ltd | Professional & scientific | 2002 |
| Accted Limited | Professional & scientific | 2013 |
| Accurate Utilities Ltd | Other services | 2007 |
| Ace Events & Development Limited | Professional & scientific | 2020 |
| Ace Lolly Limited | Manufacturing | 2014 |
| Ace Nature Ltd | Agriculture & fishing | 2022 |
| Ace of Fades Barber Chester Ltd | Other services | 2023 |
| Ace Wilding Ltd | Agriculture & fishing | 2026 |
| Aceto Agricultural Chemicals Corporation Limited | Extraterritorial | 2006 |
| ACF Direct Ltd | Wholesale & retail | 2023 |
| Acgoya Wellness Ltd | Wholesale & retail | 2025 |
| Ackner Amber Limited | Real estate | 2020 |
| Acmos U.K. Ltd. | Manufacturing | 1986 |
| Acol FI STD Ltd | Financial services | 2021 |
| Acol Gamma Fixed Interest LLP | | 2019 |
| Acol Limited | Real estate | 2018 |
| Acol Sigma LLP | | 2019 |
| Acol STD Ltd | Real estate | 2019 |
| Acorn Property Ventures Limited | Real estate | 2000 |
| Acorus Therapeutics Limited | Professional & scientific | 2000 |
| Acoustica North West Limited | Wholesale & retail | 2019 |
| Acqualda Ltd | Wholesale & retail | 2026 |
| Acrefare Estates Ltd | Real estate | 2003 |
| Acres Dairy Limited | Manufacturing | 2009 |
| Acres Farm Cheshire Ltd | Agriculture & fishing | 2024 |
| Acres Industrial Ltd | Real estate | 2025 |
| Acres Storage Ltd | Transport & logistics | 2025 |
| Acres Touring Ltd | Hospitality | 2024 |
| ACT Events Ltd | Arts & recreation | 2026 |
| Action Stunts UK Limited | Extraterritorial | 2022 |
| Actionistasx Limited | Professional & scientific | 2019 |
| Active Community Engagement Limited | Other services | 2008 |
| Active Control Solutions Limited | Professional & scientific | 2015 |
| Active Leisure Management Limited | Arts & recreation | 2017 |
| Active Quality Recruitment Limited | Professional & scientific | 1997 |
| Active Signal Box Ltd | Wholesale & retail | 2024 |
| Activity Carers Agency Ltd | Health & social care | 2018 |
| Acts Venture Ltd | Real estate | 2017 |
| ACU Consultancy Ltd | Professional & scientific | 2022 |
| Acumen Biomedical Limited | Professional & scientific | 2018 |
| Acute Consultant Care Limited | Health & social care | 2014 |
| Acute Consultants Limited | Professional & scientific | 2007 |
| Ad.Surf.Eng. Limited | Professional & scientific | 2001 |
| Adada Healthcare Group Ltd | Health & social care | 2015 |
| Adada Ltd. | Health & social care | 2017 |
| Adada Recruitment and Training Services (Arts) Ltd | Education | 2019 |
| Adam Bebbington Heating & Plumbing Ltd | Construction | 2022 |
| Adam Chippy CH1 Ltd | Hospitality | 2025 |
| Adam Clark Holdings Ltd | Financial services | 2021 |