| 64 Liverpool Road Chester Management Company Limited | Households | 2018 |
| 64H Limited Liability Partnership | | 2004 |
| 657 Consulting Ltd | Professional & scientific | 2021 |
| 66 Marketing Solutions Limited | Professional & scientific | 2022 |
| 77 Northgate Street Limited | Real estate | 2024 |
| 777 Aero Ltd | Manufacturing | 2025 |
| 777 Marketing Ltd | Professional & scientific | 2015 |
| 79/91 New Kings Road Limited | Real estate | 2005 |
| 8-18 Wentworth Mews (Pontefract) RTM Company Limited | Real estate | 2018 |
| 87 Pinner View Ltd | Real estate | 2023 |
| 899 Enterprises Limited | Financial services | 2023 |
| 8M Enterprise Limited | Wholesale & retail | 2023 |
| 8MB Ltd | Construction | 2019 |
| 9 Hunters Walk Holdings Ltd | Real estate | 2026 |
| 9 Hunters Walk Ltd | Construction | 2025 |
| 9 Princes Avenue Management Company Limited | Real estate | 2003 |
| A & a Evans Ltd | Real estate | 2023 |
| A & G Joinery (NW) Ltd | Construction | 2025 |
| A & L Performance Ltd | Wholesale & retail | 2017 |
| A & LM Clarke Ltd | Financial services | 2020 |
| A & S Contractors (NW) Limited | Construction | 2014 |
| A & S Evans Ltd | Professional & scientific | 2023 |
| A & W Joinery Limited | Construction | 2004 |
| A Beautiful Rebellion Limited | Other services | 2025 |
| A Catered Affair Limited | Wholesale & retail | 1999 |
| A J Development Consultancy Limited | Professional & scientific | 2016 |
| A J Kelly & Co (UK) Ltd | Administrative & support | 2003 |
| A J Project Management Ltd | Professional & scientific | 2022 |
| A J Shone & SON Limited | Construction | 2001 |
| A J V Consultants Limited | Administrative & support | 2020 |
| A Jahan Ltd | Wholesale & retail | 2018 |
| A Jarvis Electrical (Widnes) Limited | Construction | 2016 |
| A K N R Property Ltd | Real estate | 2025 |
| A Logan Pain Management Ltd | Health & social care | 2010 |
| A M Flight Limited | Professional & scientific | 2013 |
| A Mcdonnell Building Limited | Construction | 2019 |
| A Move Brokers Ltd | Financial services | 2016 |
| A Move Homes Limited | Real estate | 2025 |
| A Parry FS Ltd | Financial services | 2021 |
| A Price Consulting Limited | Professional & scientific | 2017 |
| A R Fearnall Limited | Agriculture & fishing | 2003 |
| A R Taxi Rentals Chester Ltd | Transport & logistics | 2026 |
| A Reef Limited | Professional & scientific | 2026 |
| A Secret Garden Landscape Design & Implementation Ltd | Administrative & support | 1999 |
| A Skip 2U Chester Limited | Water & waste | 2025 |
| A Smart Solution Ltd | Professional & scientific | 2009 |
| A T Green Ltd | Construction | 2006 |
| A Tasty Experience Ltd | Wholesale & retail | 2012 |
| A to Z Cover Ltd | Financial services | 2021 |
| A Transparent Financial Solution Limited | Financial services | 2014 |
| A Transparent Group Ltd | Financial services | 2015 |
| A V Wilding (Chester) Limited | Real estate | 1985 |
| A&A Property Builders Ltd | Real estate | 2025 |
| A&A Sahi Limited | Health & social care | 2024 |
| A&D Pubs Ltd | Hospitality | 2020 |
| A&J Project Services Limited | Real estate | 2017 |
| A&M Motorcycle Engineers Ltd | Wholesale & retail | 2017 |
| A&R Autosteer Limited | Wholesale & retail | 2025 |
| A&S Asset Management Limited | Construction | 2020 |
| A&S Step UP Properties Ltd | Real estate | 2023 |
| A&V Worx Limited | Information & communication | 2026 |
| A&Y Jenkins Ltd | Other services | 2003 |
| A-Tec Aerospace Ltd | Professional & scientific | 2026 |
| A-Z Performance Ltd | Wholesale & retail | 2019 |
| A. C. Counselling Limited | Public administration | 2000 |
| A. Day to Remember Chester Ltd. | Administrative & support | 2009 |
| A. P. 10 Ltd | Real estate | 2018 |
| A. Wall Brickwork Limited | Construction | 2003 |
| A.E.Pharma Limited | Health & social care | 2009 |
| A.M. Clearing (Transport) Limited | Transport & logistics | 1984 |
| A.M. Custom Clothing (UK) Limited | Wholesale & retail | 2014 |
| A.O.G. Paintcare Ltd | Other services | 2023 |
| A.R.M. Foundations and Groundworks Ltd | Construction | 2014 |
| A.W. Engineering (NW) Ltd | Professional & scientific | 2024 |
| A1 Autohaus Ltd | Wholesale & retail | 2022 |
| A1 Scaff Limited | Construction | 2001 |
| A2B Business Growth Ltd | Professional & scientific | 2017 |
| A55 Health & Protection Ltd | Financial services | 2026 |
| AA Decorative Events Ltd | Administrative & support | 2018 |
| AA PM Services Limited | Professional & scientific | 2021 |
| AAA & P Consulting Limited | Professional & scientific | 2015 |
| AAC Foundation CIC | Other services | 2025 |
| Aarco Nominees Limited | Professional & scientific | 1994 |
| Aarco Services Limited | Professional & scientific | 1994 |
| Aardvarker.Com Limited | Professional & scientific | 2010 |
| Aaron & Partners (Chester) Limited | Professional & scientific | 1994 |
| Aaron & Partners LLP | | 2004 |
| Aaron's Services Limited | Administrative & support | 2026 |
| Aarons Limited | Professional & scientific | 2003 |
| AAS Parts Ltd | Administrative & support | 2022 |
| AB Fire Prevention Wales Limited | Construction | 2001 |
| AB Images Limited | Other services | 2015 |
| AB Surgical Ltd | Health & social care | 2019 |
| AB Training Ltd | Education | 2015 |
| ABA Pinnacle Group Limited | Real estate | 2023 |
| Abacus Home Improvements Ltd | Construction | 2024 |
| Abacus Organic Services Limited | Administrative & support | 2001 |
| Abacus Radiology Ltd. | Health & social care | 2016 |
| Abberley Court NO.1 RTM Company Limited | Households | 2017 |
| Abberley Court NO.2 RTM Company Limited | Households | 2017 |