| Adam Flowers Advisory Ltd | Information & communication | 2026 |
| Adam J Symons Plumbing & Heating Ltd | Construction | 2021 |
| Adam Owens Financial Solutions Limited | Financial services | 2008 |
| Adam Partridge Limited | Administrative & support | 2008 |
| Adam Partridge South West Limited | Administrative & support | 2022 |
| Adams Aerial Photography Limited | Professional & scientific | 2025 |
| Adamsons Cars Ltd | Wholesale & retail | 2015 |
| Adastrapublishing Limited | Information & communication | 2021 |
| Adaugeo Rain Ltd | Administrative & support | 2021 |
| Adbecc Limited | Professional & scientific | 2025 |
| Adcote Consulting Limited | Administrative & support | 2017 |
| Adcroft Holding Inc. Limited | Professional & scientific | 2007 |
| Addanex International Limited | Administrative & support | 2022 |
| Added Value FMC Ltd | Construction | 2017 |
| Addhire Self Drive Limited | Administrative & support | 1990 |
| Additional Needs Housing Limited | Real estate | 2017 |
| Additive Housing Ltd | Real estate | 2021 |
| Address Sales and Lettings (Chester) Ltd | Real estate | 2025 |
| Addys DEL RIO Peon Limited | Health & social care | 2014 |
| Adeblinks Ltd | Health & social care | 2022 |
| Adeje Limited | Professional & scientific | 2007 |
| Adem Developments Limited | Hospitality | 2004 |
| ADG Living Limited | Construction | 2022 |
| Adhoc Worldwide Limited | Hospitality | 2020 |
| Adiuto Limited | Financial services | 2015 |
| Adrift Pictures Ltd | Arts & recreation | 2016 |
| ADS Commercial Ventures Ltd | Professional & scientific | 2026 |
| ADS Fire & Security Ltd | Administrative & support | 2020 |
| ADT Property Holdings Limited | Real estate | 2014 |
| Adta Ltd | Hospitality | 2025 |
| Advanced Fuel Solutions (Chester) Limited | Professional & scientific | 2012 |
| Advanced International Manufacturing Limited | Professional & scientific | 2015 |
| Advanced Leather Care Limited | Administrative & support | 2016 |
| Advantage Couriers Ltd | Transport & logistics | 1999 |
| Advantage Motorsport Ltd | Wholesale & retail | 2009 |
| Adventure Property Limited | Real estate | 2024 |
| Adviser Hero Limited | Information & communication | 2022 |
| AE Energy Ltd | Energy | 2023 |
| AE Global International Systems Ltd | Information & communication | 2023 |
| AE Property Management Ltd | Real estate | 2024 |
| Aeclipse Ltd | Financial services | 2014 |
| Aeee Ltd | Hospitality | 2019 |
| AEM Industry Advisory Ltd | Professional & scientific | 2024 |
| AEN PUB Co Ltd | Hospitality | 2024 |
| Aerohawk (E & I) Limited | Wholesale & retail | 1979 |
| Aeron Valley Tractors Limited | Wholesale & retail | 2002 |
| Aeroxpress Limited | Transport & logistics | 2025 |
| Aertec Solutions Limited | Construction | 2012 |
| Aesthetic Plastic Surgeons Limited | Health & social care | 2009 |
| Aesthetic Sense @ Chester Limited | Health & social care | 2014 |
| Aesthetically Insured Limited | Financial services | 2024 |
| Aestheticare Ltd | Wholesale & retail | 2024 |
| Aesthetics Chester Limited | Other services | 2015 |
| Aesthetics Telephone AI Ltd | Information & communication | 2026 |
| Aether Air Ltd | Manufacturing | 2020 |
| Affiche Store Ltd | Manufacturing | 2023 |
| Affiliate Famly Ltd | Professional & scientific | 2025 |
| Afra International Limited | Financial services | 2022 |
| Africa & Barefoot Experiences Ltd | Administrative & support | 2023 |
| African Octopus Ltd | Professional & scientific | 2010 |
| Afrozim Taste UK Limited | Wholesale & retail | 2025 |
| After School (UK) Limited | Education | 2002 |
| AG Dream Limited | Real estate | 2026 |
| AG Home Services Ltd | Administrative & support | 2018 |
| AG Roentgen Services Ltd | Health & social care | 2023 |
| AG4TECH Limited | Professional & scientific | 2020 |
| AGD Architectural Limited | Professional & scientific | 2012 |
| AGD Regeneration Ltd | Professional & scientific | 2007 |
| AGENCY97 Limited | Information & communication | 2011 |
| Agievolution Limited | Professional & scientific | 2024 |
| Agile Technologies Ltd | Wholesale & retail | 2023 |
| AGM Global Securities Limited | Administrative & support | 2024 |
| AGM Phelan Ltd | Health & social care | 2014 |
| Agri-Bits Ltd | Wholesale & retail | 2026 |
| Agrisound Limited | Manufacturing | 2020 |
| Agristo UK Ltd | Administrative & support | 2008 |
| Agroup Ltd | Wholesale & retail | 2019 |
| Agua Coolers Ltd | Wholesale & retail | 2012 |
| AH @ Thirty Two Ltd | Administrative & support | 2016 |
| AHF Electrical Ltd | Construction | 2024 |
| AHG Landscapes Ltd | Agriculture & fishing | 2018 |
| AHLX24 Ltd | Professional & scientific | 2024 |
| AHM Gardening Services Ltd | Administrative & support | 2025 |
| Ahmed’S Finest Ltd | Hospitality | 2026 |
| AI Edge Solutions Ltd | Information & communication | 2025 |
| Aijef Limited | Financial services | 2025 |
| AIM Gram Ltd | Transport & logistics | 2020 |
| Air 23 Limited | Professional & scientific | 2016 |
| Air Quality Research Ltd | Professional & scientific | 2008 |
| Airfab Ltd | Professional & scientific | 2025 |
| Airhosted Limited | Real estate | 2019 |
| Airwave Surfing Technologies Ltd | Wholesale & retail | 2015 |
| AJ & AJ Property Ltd | Real estate | 2021 |
| AJ & GM Cliff Ltd | Agriculture & fishing | 2012 |
| AJ Brown Financial Planning Ltd | Professional & scientific | 2010 |
| AJ Consulting (Chester) Ltd | Professional & scientific | 2017 |
| AJ Roofing & Guttering Ltd | Construction | 2025 |
| AJ Rowton Limited | Hospitality | 2026 |
| AJ Skyblue Ltd | Real estate | 2019 |
| AJG Software Ltd | Information & communication | 2023 |