| Dryfesdale NSA Limited | Households | 2016 |
| Dryfesdale NSH Limited | Households | 2016 |
| Dryfield Meadow Properties Ltd | Real estate | 2022 |
| Drygrounds Farm Ltd | Agriculture & fishing | 2004 |
| Dryhill Wine Ltd | Manufacturing | 2016 |
| DS Construction (Cheltenham) Limited | Construction | 2014 |
| DS Marine Contracting Limited | Construction | 2016 |
| DS Planning Services Limited | Professional & scientific | 2023 |
| DS Property (Cheltenham) Limited | Real estate | 2017 |
| DS Tutoring Limited | Education | 2019 |
| Ds&As Catering Ltd | Hospitality | 2026 |
| Dsfearn Limited | Professional & scientific | 2023 |
| DSP (UK) Limited | Wholesale & retail | 2006 |
| DSP Photography Limited | Professional & scientific | 2014 |
| Dsquare Limited | Professional & scientific | 2025 |
| DT Motors Limited | Wholesale & retail | 2015 |
| DTC Coaching & Consultancy Ltd | Professional & scientific | 2002 |
| DTM Management Ltd | Professional & scientific | 2014 |
| DU Preez Ltd | Administrative & support | 2023 |
| Duality Technologies Ltd | Administrative & support | 2024 |
| Dualstone Projects Limited | Real estate | 2025 |
| Dubbtech Ltd | Real estate | 2019 |
| Ducere Global Business School UK Limited | Education | 2018 |
| Ducklington Solar Limited | Energy | 2020 |
| Duckworth Overland Ltd | Manufacturing | 2020 |
| Dudbridge Meadows Limited | Households | 2005 |
| Dudley Court RTM Company Ltd | Households | 2020 |
| Dull Enterprises Limited | Manufacturing | 2025 |
| Dulwich Wood Investments Limited | Financial services | 1959 |
| Dunalley Street (Cheltenham) Management Company Limited | Households | 2002 |
| Dunkerton Properties LLP | | 2009 |
| Dunkerton Properties Projects Limited | Professional & scientific | 2017 |
| Dunkertons Cider Company Limited | Manufacturing | 2000 |
| Dunkertons Cider Holdings Limited | Extraterritorial | 2016 |
| Dunkertons Group Holdings Limited | Construction | 1999 |
| Dunkirk Mills Management Company Limited | Households | 1994 |
| Dunmil Care UK Limited | Health & social care | 2025 |
| Dunn's Electrical Services Limited | Professional & scientific | 2008 |
| Dunsford Properties Limited | Administrative & support | 1999 |
| Durasto Limited | Wholesale & retail | 2018 |
| Durbin Properties Limited | Real estate | 2023 |
| Durkan Cahill Limited | Professional & scientific | 2009 |
| Duskytv Ltd | Information & communication | 2014 |
| Dustbusters Contract Cleaning Services Limited | Administrative & support | 2011 |
| Dutton Property Management Limited | Real estate | 2016 |
| Dutton Referrals Limited | Professional & scientific | 2024 |
| Duża Dupa Ltd | Professional & scientific | 2024 |
| Dvijanair Ltd | Wholesale & retail | 2026 |
| DW Automotive Transport Ltd | Transport & logistics | 2020 |
| Dwellston Ltd | Administrative & support | 2025 |
| Dyback Ltd | Manufacturing | 2019 |
| Dyenamic Solutions Ltd | Extraterritorial | 2023 |
| Dyfi Sporting Limited | Arts & recreation | 2024 |
| Dykes Property Investment Ltd | Real estate | 2024 |
| Dynamic Youth Property Solutions Limited | Real estate | 2024 |
| Dynamic Youth Support Services Limited | Health & social care | 2013 |
| Dynamite Songs Limited | Information & communication | 2023 |
| Dziubas Ltd | Professional & scientific | 2019 |
| E & G Websales Limited | Wholesale & retail | 2004 |
| E & K Hairstyle Limited | Arts & recreation | 2024 |
| E B Hayward & Co Limited | Manufacturing | 2015 |
| E Basri Ltd | Transport & logistics | 2020 |
| E Estates Management AMF Limited | Other services | 2026 |
| E Estates Management Limited | Other services | 2021 |
| E G M Real Estates Limited | Real estate | 2017 |
| E H Sports Limited | Arts & recreation | 2024 |
| E M Badger Ltd | Construction | 2013 |
| E P G Services Limited | Administrative & support | 1974 |
| E Parker Precision Limited | Transport & logistics | 2025 |
| E Restaurants Limited | Hospitality | 2022 |
| E&L Jinks Ltd | Financial services | 2025 |
| E-Qs Construction Management Ltd | Professional & scientific | 2017 |
| E-Tec Recruitment Ltd. | Transport & logistics | 2019 |
| E-Volition Internet Services Ltd | Information & communication | 2001 |
| E.A.Haddrell Ltd | Hospitality | 2024 |
| E.G.Farley and Sons Limited | Construction | 2005 |
| E.G.M Homes Limited | Real estate | 2014 |
| E.O.E. Housing Limited | Hospitality | 2013 |
| E.T. Electrical Partners LLP | | 2011 |
| E7 Property Ltd | Real estate | 2024 |
| Eacanhouse Ltd | Real estate | 2024 |
| Eacanproject Ltd | Professional & scientific | 2017 |
| Eachday Shared Ltd | Wholesale & retail | 2018 |
| Eager Readers Ltd | Extraterritorial | 2023 |
| Eagle & Hodges Ltd | Professional & scientific | 2022 |
| Eagle Court Management Company Limited | Households | 2015 |
| Eagle Strategic Land Limited | Real estate | 2004 |
| Eagle Strategic Property Limited | Real estate | 2003 |
| Eagle Strategic Property NW10 Ltd | Real estate | 2020 |
| EAM Equestrian Limited | Arts & recreation | 2017 |
| Eamtree Homes Limited | Construction | 2004 |
| EAR Caddy Ltd | Education | 2025 |
| Earls Court Farm (Berkshire) Limited | Agriculture & fishing | 2022 |
| Early Life Ultrasound Centre Ltd | Health & social care | 2012 |
| Earnz Holdings Limited | Financial services | 2024 |
| Earnz PLC | Professional & scientific | 2016 |
| Earnz Property Services Limited | Construction | 2019 |
| Earth Anatomy Europe Limited | Wholesale & retail | 2012 |
| Earthstars Education & Play CIC | Education | 2019 |
| Earthy Music Limited | Arts & recreation | 2015 |