| Earwax.Co.Uk Ltd | Health & social care | 2019 |
| EAS Properties Ltd | Households | 2018 |
| Easi-Tel Limited | Information & communication | 2013 |
| East Claydon Bess Limited | Energy | 2023 |
| East Claydon DC Limited | Information & communication | 2025 |
| East Claydon Green NG Limited | Financial services | 2022 |
| East Claydon Road Solar Limited | Energy | 2024 |
| East Glos Club Coaching Limited | Arts & recreation | 2016 |
| East Gloucestershire Club Limited | Arts & recreation | 2015 |
| East Midland Contract Services Limited | Construction | 2017 |
| East Tower Properties Limited | Construction | 2022 |
| Eastabrook Architects Limited | Professional & scientific | 2002 |
| Eastabrook Properties Ltd | Real estate | 2020 |
| Eastcot Developments Ltd | Construction | 2023 |
| Eastern Business Park Limited | Real estate | 2002 |
| Eastgate SE14 (Blocks a, B, C, and D) Management Company Limited | Households | 1994 |
| Eastgate SE14 (Blocks E,F,G and H) Management Company Limited | Administrative & support | 1995 |
| Eastholm Lawns Management Limited | Households | 1980 |
| Eastleach Limited | Real estate | 2016 |
| Easton Grey Estate Limited | Real estate | 1962 |
| Eastwood & Co Developments Ltd | Construction | 2025 |
| Eastwood Pest Control Limited | Agriculture & fishing | 2013 |
| Easy Indian Cookbook Ltd | Information & communication | 2024 |
| Easy Wireless Hire Ltd | Administrative & support | 2018 |
| EAT the Bird Ltd | Hospitality | 2018 |
| Eaton Property Maintenance Limited | Construction | 2003 |
| EB Engineering UK Limited | Professional & scientific | 2005 |
| EB Machinery Limited | Wholesale & retail | 2014 |
| Ebdons of Cheltenham Limited | Wholesale & retail | 2011 |
| Eberto Wester Limited | Real estate | 2024 |
| Eboknova Ltd | Professional & scientific | 2022 |
| EBS Design Limited | Manufacturing | 2015 |
| Ecctis Limited | Information & communication | 1989 |
| ECG Agri Limited | Wholesale & retail | 2022 |
| Echo Partners Limited | Professional & scientific | 2024 |
| Eckley Consulting Ltd | Professional & scientific | 2026 |
| Eclipse Business Supplies Limited | Wholesale & retail | 2023 |
| Ecliptic Dynamics Limited | Information & communication | 2019 |
| Eco Traveler. Ltd. | Wholesale & retail | 2016 |
| Eco-Wood Imports Limited | Wholesale & retail | 2023 |
| Ecobinsolutionsuk Ltd | Manufacturing | 2025 |
| Ecoclean Carpet & Upholstery Care Limited | Administrative & support | 2019 |
| Ecoclean Environmental Limited | Administrative & support | 2015 |
| Ecoclean Pest Control Ltd | Administrative & support | 2025 |
| Ecodian Digital Limited | Wholesale & retail | 2024 |
| Ecodian Holdings Limited | Financial services | 2023 |
| Ecoeats Ltd | Information & communication | 2024 |
| Ecopoint Solutions Ltd | Administrative & support | 2024 |
| Ecoswash Limited | Wholesale & retail | 2022 |
| Ecpc Software Ltd | Information & communication | 2025 |
| Ecpf Ltd | Health & social care | 2024 |
| ED and Sarna Ltd | Wholesale & retail | 2015 |
| ED DE Giles Racing LLP | | 2015 |
| Edad Limited | Education | 2014 |
| EDB Consultants Ltd | Professional & scientific | 2025 |
| Edcentral Limited | Information & communication | 2007 |
| Edco Latam UK Ltd | Professional & scientific | 2024 |
| Eddison Properties Ltd | Real estate | 2025 |
| Eden Holme Management Company Limited | Households | 1990 |
| Eden One Group Ltd | Wholesale & retail | 2019 |
| Edenhill Productions Ltd | Information & communication | 2022 |
| Edenity Limited | Professional & scientific | 2024 |
| Edgcumbe Ltd | Construction | 2023 |
| Edge Architectural & Building Consultants Ltd | Professional & scientific | 2021 |
| Edge Design Workshop Limited | Professional & scientific | 2003 |
| Edge Research Limited | Professional & scientific | 2022 |
| Edgebuild Construction Ltd | Construction | 2018 |
| Edgecombe Holdings (Glos) Ltd | Wholesale & retail | 2026 |
| Edgecombe Management Consulting Limited | Professional & scientific | 2021 |
| Edgecombe Property Investment Ltd | Real estate | 2017 |
| Edging (UK) Limited | Manufacturing | 2014 |
| Edio Education Ltd | Education | 2007 |
| Edison Trading Limited | Financial services | 2025 |
| Edit Brands Limited | Financial services | 2026 |
| EDIT58 Limited | Wholesale & retail | 2015 |
| Edmonds Aviation Limited | Transport & logistics | 2025 |
| Edmonstone House (Cheltenham) Management Company Limited | Households | 2024 |
| Education-Training Ltd | Administrative & support | 2007 |
| Edward Bick Anaesthesia Limited | Health & social care | 2012 |
| Edward Elgar Publishing Limited | Information & communication | 1986 |
| Edward Gilder & Co Limited | Transport & logistics | 2001 |
| Edwards & Company (Glos) Ltd | Construction | 2000 |
| EE Hospitality Ltd | Professional & scientific | 2023 |
| Eevolv Holdings Limited | Financial services | 2021 |
| Eevolv Ltd | Professional & scientific | 2018 |
| EGM Capital Limited | Real estate | 2025 |
| EGN Golf Ltd | Wholesale & retail | 2020 |
| EHG Developments Ltd | Real estate | 2023 |
| EHG Holdings Limited | Real estate | 2021 |
| EHP Mechanical Limited | Construction | 2020 |
| EI Partners Ltd | Professional & scientific | 2026 |
| Eight Legal Limited | Professional & scientific | 2010 |
| Eivissa GLO Ltd | Other services | 2025 |
| EJ Lake Properties Limited | Real estate | 2025 |
| EJ Mcgurk Limited | Financial services | 2025 |
| EJH Consulting Ltd | Professional & scientific | 2005 |
| EJM 08 Limited | Extraterritorial | 2018 |
| EJM 87 Limited | Construction | 2020 |
| Ekin Coaching and Development Limited | Health & social care | 2025 |
| Ekollon Limited | Wholesale & retail | 2011 |