| Delucia, Casa & Bere Ltd | Real estate | 2019 |
| Deluxe Nails Cheltenham Ltd | Other services | 2025 |
| Deluxetrade Ltd | Professional & scientific | 2025 |
| Demand Analysis Limited | Information & communication | 2012 |
| Demaya Ltd | Transport & logistics | 2026 |
| Demi Pranic Healing Ltd | Health & social care | 2026 |
| Demon Pixels Limited | Extraterritorial | 2010 |
| Dencare Holdings Limited | Financial services | 2017 |
| Dendy Ltd | Real estate | 2021 |
| Denham Lodge (Cheltenham) Management Company Limited | Real estate | 1997 |
| Denis Woulfe Consulting Limited | Professional & scientific | 2017 |
| Denman Property Ltd | Real estate | 2022 |
| Denmark House Management Company Limited | Households | 2005 |
| Denmark Road (Gloucester) Limited | Construction | 2022 |
| Densems (Holdings) Limited | Real estate | 1952 |
| Dental and Medical Wealth Management Limited | Financial services | 2011 |
| Dental Audio Notes Limited | Information & communication | 2020 |
| Dental Dimensions Limited | Health & social care | 2006 |
| Dental Network Agency Ltd | Administrative & support | 2024 |
| Dentalkart Distribution UK Ltd | Manufacturing | 2025 |
| Dentex Assets Limited | Health & social care | 2017 |
| Dentex Clinical Limited | Financial services | 2018 |
| Dentex Finco Limited | Financial services | 2018 |
| Dentex Healthcare Group Limited | Health & social care | 2014 |
| Dentex Manco Limited | Administrative & support | 2018 |
| Dentex Nominees Limited | Financial services | 2021 |
| Dentex Practices Limited | Health & social care | 2017 |
| Denton Lodge Limited | Households | 1996 |
| Denwell Mini Coaches Limited | Transport & logistics | 1999 |
| Denyer Electrical Services Limited | Construction | 2020 |
| Depot 65 Limited | Wholesale & retail | 2023 |
| Derbidge Brothers Limited | Administrative & support | 2015 |
| Derivation Limited | Other services | 1998 |
| Derp Studios Ltd | Manufacturing | 2012 |
| Desborough Solar and Battery Storage II Limited | Energy | 2023 |
| Desborough Solar and Battery Storage Limited | Energy | 2023 |
| Desert and Villaricos Properties Limited | Real estate | 2014 |
| Desert Ridge Estates LLP | | 2014 |
| Desi Trill Ltd | Information & communication | 2023 |
| Design and Building Services Ltd | Construction | 2023 |
| Design and Fit-Out Limited | Professional & scientific | 2000 |
| Design ANY Time Ltd | Wholesale & retail | 2025 |
| Design AV Europe Limited | Professional & scientific | 2008 |
| Design Capsule Limited | Manufacturing | 2025 |
| Design CDM Ltd | Professional & scientific | 2024 |
| Design Flooring Cirencester Limited | Construction | 2016 |
| Design Installation Service Limited | Construction | 1973 |
| Design Punch Limited | Information & communication | 2018 |
| Design-Mind Limited | Professional & scientific | 2008 |
| Design-N-Build Ltd. | Construction | 2017 |
| Designed by Anouska Ltd | Manufacturing | 2017 |
| Desirable Childcare Limited | Education | 2006 |
| Desito Ltd | Information & communication | 2014 |
| Despatch Pro Limited | Information & communication | 2016 |
| Deuce Deco Ltd | Administrative & support | 2015 |
| Developers Boardroom Limited | Education | 2018 |
| Devereaux Court Management (NO 3) Limited | Households | 1994 |
| Devereux & Co. Limited | Professional & scientific | 2001 |
| Deverson Scaffolding Services Ltd | Construction | 2012 |
| Deviant Hearts INK Ltd | Information & communication | 2022 |
| Devil MUD Run Limited | Arts & recreation | 2014 |
| Devil Sold HIS Soul Ltd | Arts & recreation | 2020 |
| Devine Builders Tewkesbury Ltd | Construction | 2023 |
| Devis Ductwork Services Ltd | Construction | 2019 |
| Devonshire House Residents Association Limited | Professional & scientific | 1993 |
| Dewebuild Ltd | Construction | 2024 |
| Dexi Candles Ltd | Wholesale & retail | 2024 |
| Deya Brewing Company Limited | Manufacturing | 2015 |
| Dezac Limited | Professional & scientific | 1997 |
| DFL Sales Consulting Limited | Professional & scientific | 2011 |
| DG8 Consulting LLP | | 2025 |
| DGP Professionals Limited | Professional & scientific | 2011 |
| Dgrt Educational Consultancy Ltd | Education | 2014 |
| DHA Holdings Limited | Financial services | 2023 |
| Dhaa Property Ltd | Real estate | 2026 |
| Dhelta Limited | Information & communication | 2025 |
| Dhillon Property Investment Group Ltd | Real estate | 2025 |
| DHL Developments Limited | Construction | 2019 |
| Di.Renders Limited | Information & communication | 2026 |
| Diagem Trading Limited | Professional & scientific | 2006 |
| Dial-A-Cab (Cheltenham) Limited | Transport & logistics | 2014 |
| Dialog Music Ltd | Arts & recreation | 2018 |
| Dialogix Limited | Professional & scientific | 2003 |
| Diamond Chef Recruitment Ltd | Administrative & support | 2022 |
| Diamond Group (Holdings) Ltd | Administrative & support | 2015 |
| Diamond Skies Explorers Ltd | Administrative & support | 2024 |
| Diamond Skies Travel Ltd | Administrative & support | 2018 |
| Diana AI Ltd | Information & communication | 2025 |
| Diass Limited | Information & communication | 2002 |
| Dickens Wealth Management Limited | Professional & scientific | 2024 |
| DIG Cat Dog Limited | Education | 2017 |
| Digbeth Enterprises Limited | Real estate | 2024 |
| Digby Morris Ltd | Administrative & support | 2003 |
| Digi Broadcast and Marketing Limited | Information & communication | 2022 |
| Digi Yeah Ltd | Information & communication | 2020 |
| Digiati Tecnologia Ltd | Information & communication | 2020 |
| Digital APE Training Ltd | Professional & scientific | 2019 |
| Digital Assured Ltd | Administrative & support | 2011 |
| Digital Conscience Ltd | Information & communication | 2022 |
| Digital Discipline Limited | Wholesale & retail | 2018 |