| Duncan Ward Legal Services Limited | Professional & scientific | 2019 |
| Duncan-Beers Bloodstock LLP | | 2022 |
| Dunridge Limited | Administrative & support | 2002 |
| Dunstal Field Residents Association Company Limited | Other services | 1974 |
| Dunwood Court Management Limited | Households | 1981 |
| Dunya Driving Academy Ltd | Education | 2025 |
| DUO MJD Limited | Professional & scientific | 2017 |
| DUO Property Limited | Real estate | 2016 |
| Durable Partners Ltd | Professional & scientific | 2024 |
| Duraj.Space Ltd | Health & social care | 2018 |
| Durga Estates Ltd | Real estate | 2022 |
| Durham Magneto Optics Ltd | Manufacturing | 2002 |
| Durman Stearn (Civil Engineering) Limited | Construction | 1979 |
| Durman Stearn Holdings Limited | Financial services | 2014 |
| Durman Stearn Plant Limited | Construction | 2014 |
| Duroliponte Group Limited | Professional & scientific | 2017 |
| Dusk Enterprises Limited | Wholesale & retail | 2021 |
| Duskiziro Limited | Hospitality | 2020 |
| Dutair Ltd | Manufacturing | 2024 |
| Dutton Composites Ltd | Manufacturing | 2019 |
| Duudeo Services Ltd | Hospitality | 2024 |
| Duxford Automotive Limited | Wholesale & retail | 2017 |
| Duxford Aviation Society | Arts & recreation | 1982 |
| Duxford Limited | Other services | 2017 |
| Duxford Motors Ltd | Wholesale & retail | 2026 |
| Duxford Radio Limited | Arts & recreation | 2017 |
| Duxford Scrumpy Company Limited | Manufacturing | 2016 |
| Duxford Taverns Limited | Hospitality | 2022 |
| Duxford Training Limited | Administrative & support | 2017 |
| DV Peakcore Ltd | Manufacturing | 2026 |
| DVH Composites Ltd | Manufacturing | 2023 |
| DVL Technology Limited | Information & communication | 2008 |
| Dvsdesign Ltd | Construction | 2021 |
| DW Residential (Cambridge) Limited | Real estate | 2018 |
| Dw&Rw Services Ltd | Other services | 2021 |
| Dwell Haus Ltd | Construction | 2020 |
| Dwelleth Aide Ltd | Administrative & support | 2024 |
| Dyadaxis Ltd | Professional & scientific | 2024 |
| Dyason Developments (Gills Hill) Ltd | Construction | 2024 |
| Dyason Developments (West Wratting) Ltd | Construction | 2024 |
| Dyason Developments Ltd | Construction | 2017 |
| Dyason Devhold Company Limited | Households | 2024 |
| Dycraft Ltd | Construction | 2018 |
| Dyecast Limited | Households | 1987 |
| Dylf Ltd | Wholesale & retail | 2024 |
| Dynamic DUO Dog Training Ltd | Other services | 2025 |
| Dynamic Environment Solutions Ltd | Professional & scientific | 2025 |
| Dynamics Impact Group Ltd | Professional & scientific | 2024 |
| Dynamiq Tech Limited | Information & communication | 2019 |
| Dynel Technology Limited | Manufacturing | 2021 |
| Dyspraxia UK Limited | Health & social care | 2015 |
| DZ Gordon Capital Limited | Financial services | 2024 |
| DZP Technologies Limited | Professional & scientific | 2008 |
| E & V Trading Dancewear Limited | Wholesale & retail | 2015 |
| E C Autocare LLP | | 2018 |
| E Ltd | Extraterritorial | 2026 |
| E M Ashby Limited | Real estate | 1972 |
| E M Spratt Unlimited | Agriculture & fishing | 2010 |
| E Seng International Co., Limited | Wholesale & retail | 2025 |
| E W LEE Holdings Limited | Financial services | 2019 |
| E W LEE Limited | Financial services | 2004 |
| E&A Property Group Limited | Real estate | 2019 |
| E&F Property Services Limited | Real estate | 2017 |
| E&N Decor Extra Ltd | Construction | 2014 |
| E-Aero Ltd | Manufacturing | 2024 |
| E-Fine Industrial Co., Ltd | Wholesale & retail | 2023 |
| E-Pay Logistics Ltd | Information & communication | 2012 |
| E-Statiq Limited | Manufacturing | 2025 |
| E. & P. Construction Limited | | 1985 |
| E.A.Q. Limited | Wholesale & retail | 1999 |
| E.C.O Window Cleaning Ltd | Other services | 2019 |
| E.H.Lee Limited | Information & communication | 1941 |
| E.J. Johnson & SON (Cambridge) Limited | Administrative & support | 1999 |
| E.R.Shopfitters Ltd. | Construction | 2012 |
| EA Sports Agent Ltd | Arts & recreation | 2019 |
| Eagle Genomics India Holdings Ltd | Information & communication | 2019 |
| Eagle Haulage Ltd | Transport & logistics | 2015 |
| Earith Plant Limited | Wholesale & retail | 1969 |
| Earl of Beaconsfield Cambridge Ltd | Hospitality | 2025 |
| Earls Hill Place Limited | Real estate | 2002 |
| Earmill Studios Ltd | Arts & recreation | 2013 |
| Earth & Baby Ltd | Wholesale & retail | 2020 |
| Earth & Mind CIC | Administrative & support | 2023 |
| Earth Positive Consulting Ltd | Professional & scientific | 2021 |
| Earthblink Limited | Information & communication | 2014 |
| Earthnity Limited | Information & communication | 2025 |
| Earthworks-Jobs.Com Limited | Professional & scientific | 2002 |
| Ease 26 Limited | Hospitality | 2023 |
| Ease Pilates Ltd | Arts & recreation | 2021 |
| Easi-Law Limited | Other services | 2000 |
| Easify Ltd | Information & communication | 2025 |
| Easili Ltd | Construction | 2013 |
| East & Willow Ltd | Agriculture & fishing | 2020 |
| East Anglia Surveyors Ltd | Administrative & support | 2017 |
| East Anglia Systema Ltd | Professional & scientific | 2026 |
| East Anglia Ultrasound Services Ltd | Health & social care | 2012 |
| East Anglia Wolfside FC Academy Ltd | Arts & recreation | 2026 |
| East Anglia Youth Rowing Ltd | Education | 2022 |
| East Anglia's Children's Hospices | Health & social care | 1998 |
| East Anglia's Children's Hospices (Trading) Limited | Health & social care | 2014 |