| Rooney & Roberts Management Services Ltd | Professional & scientific | 2021 |
| Roseleigh Hotel Limited | Hospitality | 2003 |
| Rosscorium Limited | Professional & scientific | 2023 |
| Round Hill Outdoor Adventures Limited | Education | 2017 |
| RSM Psychology Limited | Health & social care | 2023 |
| RTG Developments Limited | Real estate | 2001 |
| Rubber Duck Property Ltd | Real estate | 2025 |
| Rufloreo Holdings Ltd | Financial services | 2026 |
| Rufloreo Property Ltd | Real estate | 2026 |
| Rumpus Resort Limited | Wholesale & retail | 2007 |
| Running Ltd | Arts & recreation | 2024 |
| Rusty Farming Limited | Agriculture & fishing | 2005 |
| Rusty Holdings Limited | Financial services | 2022 |
| Ryan Adshead Property Ltd | Real estate | 2024 |
| S a Garlick Ltd | Transport & logistics | 2016 |
| S J Boyd Haulage Limited | Transport & logistics | 2016 |
| S P Davies Consulting Limited | Information & communication | 2017 |
| S P Davies Property Limited | Real estate | 2025 |
| S P Property Traders Ltd | Real estate | 2018 |
| S&L Energy Ltd | Construction | 2022 |
| S&R Holiday Property Management Limited | Real estate | 2025 |
| S. Booth Millward Ltd | Hospitality | 1997 |
| S.A.Training Solutions Limited | Education | 2026 |
| Safeguard Sprinkler Systems Limited | Construction | 2022 |
| Saltlick Limited | Hospitality | 2024 |
| SAM Hockenhull Ltd | Other services | 2022 |
| SAM Rodger Groundworks Ltd | Construction | 2024 |
| Sammie Ramsay Beauty Limited | Other services | 2024 |
| Sampson Design & Construction Ltd. | Administrative & support | 2008 |
| Samudra Buddhist Centre | Other services | 2000 |
| Sapouma Cleaning Limited | Administrative & support | 2025 |
| SAR Paramedics Ltd. | Health & social care | 2016 |
| Sascar Limited | Professional & scientific | 2000 |
| SAW Consult Ltd | Professional & scientific | 2025 |
| Saxon Security High Peak Ltd | Administrative & support | 2013 |
| SBF Mechanic Tuning and Remaps Ltd | Manufacturing | 2021 |
| SBL Consultants Ltd | Professional & scientific | 2025 |
| Scentra Fragrances Ltd | Wholesale & retail | 2026 |
| SCJ Construction Limited | Construction | 2025 |
| Scope Medical Products Limited | Health & social care | 2001 |
| Scriptoria Illuminate Limited | Information & communication | 2022 |
| Scythion Limited | Professional & scientific | 2026 |
| SDN Crafting Change Ltd. | Construction | 2021 |
| SE7EN Management Consultants Limited | Professional & scientific | 2007 |
| Sea ID Ltd | Other services | 2021 |
| Sea Kebab Ltd | Hospitality | 2025 |
| Seasons Cafe Limited | Hospitality | 2019 |
| Selden Research Limited | Manufacturing | 1970 |
| Selene Clinical Research Ltd | Professional & scientific | 2025 |
| Sella Properties Limited | Administrative & support | 1972 |
| Seltaeb Publishing Limited | Administrative & support | 2017 |
| Semester Recruitment Limited | Administrative & support | 2005 |
| Sentinel Screening Ltd | Information & communication | 2026 |
| Serpentine Court Management Company Limited | Households | 2007 |
| Serpentine Nursery School Limited | Education | 2006 |
| Sett Valley Cycles Limited | Wholesale & retail | 2012 |
| SEX Addiction and Psychosexual Therapy Services Limited | Health & social care | 2016 |
| SFS Joinery Limited | Construction | 2015 |
| Shakespeare Garage Limited | Wholesale & retail | 2019 |
| Shallow Grange Limited | Agriculture & fishing | 2004 |
| Shann Engineering Ltd | Wholesale & retail | 2026 |
| Shared Learning Limited | Information & communication | 2017 |
| Sheffield Shine Limited | Administrative & support | 2022 |
| Sheikhs Properties Limited | Real estate | 2023 |
| Sheldon Landscape Forestry Limited | Agriculture & fishing | 2021 |
| Shelton Grill Limited | Hospitality | 2024 |
| Shining Ford Consulting Limited | Professional & scientific | 2024 |
| Shooting Range Safety Services Ltd | Professional & scientific | 2023 |
| Sigma 6 Limited | Other services | 2016 |
| Signature Stone Worktops Ltd | Professional & scientific | 2024 |
| Signs of Light Ltd | Manufacturing | 2021 |
| Sijay Property Consultants Limited | Households | 2016 |
| Silent Brew Co. Limited | Manufacturing | 2022 |
| Silver FOX Training Ltd | Education | 2011 |
| Silverlands Park Management Company (Buxton) Limited | Hospitality | 1989 |
| Silveroo Holdings Ltd | Financial services | 2024 |
| Silverwood Gilson Limited | Professional & scientific | 2015 |
| Simon Paul Property Holdings Ltd | Financial services | 2025 |
| Sinbro Limited | Real estate | 2016 |
| SJ Briggam Services Limited | Professional & scientific | 2025 |
| SJ Events and Catering Ltd | Hospitality | 2022 |
| SJB Contracting (Derbyshire) Limited | Construction | 2014 |
| SK Leisure Properties Limited | Real estate | 2023 |
| SKI Concierge Limited | Administrative & support | 2025 |
| SKI Nova Ltd | Arts & recreation | 2019 |
| Skyfall Heights Limited | Hospitality | 2025 |
| Slackwater Co Limited | Administrative & support | 2025 |
| Slap Adventures Ltd | Arts & recreation | 2016 |
| Slater Financial Ltd | Financial services | 2009 |
| Smarter Packaging Solutions Ltd | Professional & scientific | 2019 |
| Smith and Walters Ltd | Construction | 2024 |
| Smith Sisters Ltd | Hospitality | 2021 |
| Smiths Units Limited | Transport & logistics | 2025 |
| Snap Labs Ltd | Professional & scientific | 2022 |
| Sneha PVT Ltd | Hospitality | 2026 |
| SNS Site Services Ltd | Manufacturing | 2022 |
| Social Sports Ltd | Arts & recreation | 2022 |
| Soho Stampings Limited | Manufacturing | 1975 |
| SOLUTIONZ4 Limited | Professional & scientific | 2010 |
| Sottobella Kennels Ltd | Agriculture & fishing | 2026 |