| Drainhub Oxford Limited | Water & waste | 2025 |
| Drakon Group Limited | Administrative & support | 2016 |
| DRC Healthcare Limited | Health & social care | 2015 |
| Dream Song Pubs Ltd | Hospitality | 2024 |
| Dreamloom Ltd | Wholesale & retail | 2025 |
| Dreams R V Centre Limited | Administrative & support | 2003 |
| Dribble and Dollop Ltd | Construction | 2025 |
| Drirug Limited | Wholesale & retail | 2019 |
| Drive & Co Events Ltd | Arts & recreation | 2026 |
| Drives Online Limited | Wholesale & retail | 2014 |
| Driving Ambitions (UK) Limited | Extraterritorial | 1997 |
| Drumseal Limited | Professional & scientific | 1994 |
| DT3K Ltd | Administrative & support | 2024 |
| DTF Express Ltd | Manufacturing | 2026 |
| Duggan Faria Limited | Wholesale & retail | 2022 |
| Dukes Fire Safety Limited | Other services | 2016 |
| Dulla Traders Ltd | Wholesale & retail | 2025 |
| Dumbleton & SON (Joiners) Limited | Construction | 1988 |
| Dura Vista Ltd | Wholesale & retail | 2023 |
| Durbbs Ltd | Information & communication | 2016 |
| Dust Buster Brigade Ltd | Administrative & support | 2024 |
| Dvellup Limited | Real estate | 2024 |
| DVP Property Group Limited | Real estate | 2021 |
| DW Creative Limited | Professional & scientific | 2019 |
| Dynamic Ropes and Rigging Ltd | Information & communication | 2014 |
| DYS CLO Limited | Wholesale & retail | 2025 |
| E Singer Equine Orthopaedics and Surgery Limited | Professional & scientific | 2017 |
| E&A Service Solutions Ltd | Professional & scientific | 2023 |
| E&P Services and Maintenance Limited | Construction | 2023 |
| E-Power Generators UK Limited | Manufacturing | 2023 |
| E. P. B. Holdings Limited | Extraterritorial | 1997 |
| E.G. Printers (Packaging) Limited | Manufacturing | 1991 |
| E.I.P. Properties Ltd | Real estate | 2024 |
| E.P.Barrus (Property) Limited | Real estate | 1997 |
| E.P.Barrus Limited | Transport & logistics | 1917 |
| E1 Trading Ltd | Wholesale & retail | 2025 |
| E10 Ltd | Construction | 2026 |
| E7 Programmes Ltd | Professional & scientific | 2024 |
| Eagle 95 Electrician Ltd | Extraterritorial | 2018 |
| Eagle Family Ltd | Real estate | 2020 |
| Eagle Graphics (Printers) Limited | Manufacturing | 2000 |
| Early Years First AID Ltd | Administrative & support | 2016 |
| Early Years Staffroom Ltd | Education | 2017 |
| Earthed Electrical (Southern) Limited | Construction | 2015 |
| Earthed Services Ltd | Construction | 2024 |
| Earthing Revolution Ltd | Wholesale & retail | 2022 |
| Easy Box Logistics Limited | Transport & logistics | 2021 |
| Easy Buy Global Ltd | Wholesale & retail | 2018 |
| Ebmourio Investments LLP | | 2023 |
| Ebmourio Property Investments Limited | Real estate | 2023 |
| Ecclesia Tours Limited | Administrative & support | 2023 |
| Eceipt Limited | Information & communication | 2025 |
| Echomind AI Technologies Limited | Health & social care | 2025 |
| Echostream Limited | Information & communication | 2024 |
| Eclipta Solutions Ltd | Information & communication | 2025 |
| Eco Professional Cleaning Service Ltd | Administrative & support | 2022 |
| Ecorondix Ltd | Wholesale & retail | 2025 |
| ED Kennedy Consultancy Limited | Professional & scientific | 2020 |
| Eday's Fishmonger Ltd | Wholesale & retail | 2020 |
| Eddell & Co Limited | Professional & scientific | 2021 |
| Eden Dwellings Ltd | Real estate | 2022 |
| Edens Consulting Ltd | Construction | 2012 |
| Edge Consulting (Oxford) Ltd | Professional & scientific | 2021 |
| EDN Consulting Ltd | Health & social care | 2018 |
| Eduard Esanu Ltd | Transport & logistics | 2019 |
| Eduqa Ltd | Education | 2024 |
| Edward Grace Property Ltd | Real estate | 2021 |
| Edwin Douglas Limited | Education | 2019 |
| Edyzav Trans Ltd | Transport & logistics | 2018 |
| Efeed Limited | Manufacturing | 2011 |
| Effectiv AI UK Limited | Wholesale & retail | 2021 |
| EGR International Ltd | Professional & scientific | 2019 |
| EJ Social Ltd | Hospitality | 2019 |
| EJB Property Partners Ltd | Real estate | 2025 |
| EJS Financial Services Limited | Financial services | 2016 |
| EKO Security Ltd | Administrative & support | 2016 |
| Ekta Global Immigration Advisers Ltd | Professional & scientific | 2026 |
| Ekta Holdings Ltd | Professional & scientific | 2021 |
| Electrical Insulators and Supports Limited | Wholesale & retail | 2000 |
| Elegance With Edge Ltd | Real estate | 2023 |
| Element 43 Consulting Limited | Information & communication | 2017 |
| Elemental Green Design CIC | Professional & scientific | 2025 |
| Elephas BA Limited | Health & social care | 2024 |
| Elevatebuild Construction Services Ltd | Construction | 2023 |
| ELF Tech Ltd | Real estate | 2020 |
| Elfgrange Limited | Hospitality | 2005 |
| Elgin Investments LLP | | 2014 |
| Eligius Services Limited | Water & waste | 2019 |
| Elise Devon Ltd | Construction | 2026 |
| Elite Aesthetics by Roxie Ltd. | Other services | 2018 |
| Elite Options Ltd | Construction | 2026 |
| Eliza Landscapes Limited | Other services | 2020 |
| Eliza MAY Ltd | Professional & scientific | 2017 |
| Ellehtay Ltd | Wholesale & retail | 2025 |
| Ellice Limited | Construction | 2018 |
| Elliot Consulting Limited | Professional & scientific | 2020 |
| Elmgreen Estates Ltd | Real estate | 2002 |
| Elmilock Limited | Wholesale & retail | 2024 |
| ELN London Ltd | Wholesale & retail | 2023 |
| ELS Air & Ocean Ltd | Transport & logistics | 2011 |