| ELU Limited | Professional & scientific | 2020 |
| Emanjo Holdco Ltd | Construction | 2018 |
| Emay Business Ltd | Wholesale & retail | 2021 |
| Emitom Design and Build Ltd | Construction | 2017 |
| Emmerson Contracting Ltd | Construction | 2025 |
| Emmerson Law Limited | Professional & scientific | 2009 |
| Empire Scaffolding Oxfordshire Limited | Construction | 2025 |
| Empowered Supported Living Ltd | Administrative & support | 2021 |
| Empyrian Ltd | Wholesale & retail | 2019 |
| EMS Controls Limited | Construction | 2010 |
| Endillion Limited | Real estate | 2018 |
| Endurance Rally Association Ltd | Administrative & support | 2018 |
| Energy & Resources Ltd | Professional & scientific | 2020 |
| Engineering Rationale Limited | Professional & scientific | 2013 |
| Engineering Trust Training Limited | Education | 1989 |
| Engraving LAB Ltd | Manufacturing | 2022 |
| Ensco 1366 Limited | Professional & scientific | 2020 |
| Enter-At Limited | Information & communication | 2016 |
| EP Marketing Logistics Limited | Administrative & support | 2010 |
| Epayd Solution Ltd | Administrative & support | 2018 |
| Epinomos Ltd | Information & communication | 2026 |
| Epiphany Enterprises Limited | Professional & scientific | 2014 |
| Epsilon Marketing Ltd | Information & communication | 2020 |
| Epsom Property Holdings Limited | Real estate | 2020 |
| Epynt Co. Ltd | Manufacturing | 2023 |
| EQ Property Holdings Limited | Real estate | 2021 |
| EQ Services & Consultancy Limited | Extraterritorial | 2011 |
| Equate Services Limited | Arts & recreation | 1993 |
| Equation Limited | Administrative & support | 2025 |
| Equation Recruitment Limited | Administrative & support | 2009 |
| Equicheck International Limited | Information & communication | 2025 |
| Equilibrium Advisors Limited | Professional & scientific | 2024 |
| Equine BIO Genie Ltd | Administrative & support | 2020 |
| Equine BIO Genie Services Limited | Administrative & support | 2022 |
| ERA Consulting Group Ltd | Information & communication | 2024 |
| Es-Tech Network Solutions Limited | Information & communication | 2002 |
| Esbr Hospitality Ltd | Hospitality | 2025 |
| Esker Landscapes Ltd | Professional & scientific | 2025 |
| Esnath Samasuwo Services Limited | Health & social care | 2018 |
| Especially AMY Limited | Professional & scientific | 2013 |
| Espley Design Ltd | Construction | 2021 |
| Esport+Cars Virtual Race Team Ltd | Professional & scientific | 2016 |
| Espresso Architects Ltd | Professional & scientific | 2010 |
| Essentar Limited | Extraterritorial | 2008 |
| Essenza Lumi Limited | Wholesale & retail | 2025 |
| Estlat Ltd | Transport & logistics | 2025 |
| Estmanco (Besford and Dinmont) Limited | Real estate | 1979 |
| Estmanco (Chelsiter Court) Limited | Households | 1979 |
| Estoback Limited | Professional & scientific | 2005 |
| Estrella Systems Ltd | Information & communication | 2002 |
| Ethical IT Services Limited | Information & communication | 2019 |
| Eurocell Recruitment Limited | Administrative & support | 2014 |
| Europa Invest Ltd | Real estate | 2023 |
| European Engineering Group Limited | Extraterritorial | 2007 |
| Euxton Properties LLP | | 2023 |
| Evans Investment Property Limited | Real estate | 2023 |
| Evans Jumps Limited | Manufacturing | 2014 |
| Evening Breeze (Nordic) Limited | Wholesale & retail | 2022 |
| Event Medical Consultancy Limited | Health & social care | 2010 |
| Event Utilities Limited | Water & waste | 2016 |
| Evidentify Ltd | Professional & scientific | 2024 |
| Evolito Battery Systems Ltd | Professional & scientific | 2022 |
| Evolito Ltd | Manufacturing | 2018 |
| Evolve MA Ltd | Arts & recreation | 2019 |
| Evolve OR DIE Limited | Arts & recreation | 2014 |
| Exadoctor Ltd | Information & communication | 2025 |
| Excalibur Properties Ltd | Real estate | 2019 |
| Excel Financial Consulting Limited | Professional & scientific | 2007 |
| Exchange Communications Group Limited | Financial services | 2014 |
| Exec Support Solutions Limited | Administrative & support | 2018 |
| Execufin Advisory Ltd | Information & communication | 2025 |
| Exeter Holdings Ltd | Real estate | 2025 |
| EXF1 Ltd | Professional & scientific | 2017 |
| Eximpex International Ltd | Wholesale & retail | 2004 |
| Expense Control LLP | | 2007 |
| Expert Logistics Solutions Limited | Transport & logistics | 2009 |
| Explore Oxford Tours Limited | Administrative & support | 2005 |
| Explorershub Ltd | Administrative & support | 2017 |
| Express BBQ Ltd | Hospitality | 2025 |
| Express Letting and Property Management (2009) Ltd | Real estate | 2009 |
| Express Plumbing & Heating Ltd | Construction | 2021 |
| Express Your Creative Ltd | Administrative & support | 2021 |
| EXPRESS1 Ltd | Wholesale & retail | 2025 |
| Exscien Training Ltd | Information & communication | 2006 |
| Exsell Academy UK Ltd | Education | 2026 |
| Extractus Ltd | Professional & scientific | 2023 |
| Extreme Environmental Services Limited | Administrative & support | 2000 |
| EZ Charge Ltd | Manufacturing | 2020 |
| F M Comp Ltd | Professional & scientific | 2022 |
| F&F Property Holdings Limited | Real estate | 2025 |
| F-Cal Support Services Limited | Extraterritorial | 2015 |
| F. Eagles Limited | Wholesale & retail | 1996 |
| Fabal UK Ltd | Wholesale & retail | 2020 |
| Faces by FLO Ltd | Other services | 2025 |
| Fairfax Retail Group Ltd | Wholesale & retail | 2022 |
| Fairfield House Hotel (AYR) Limited | Hospitality | 1993 |
| Fairfield House Hotel Limited | Financial services | 2022 |
| Fairford Heating Limited | Construction | 2024 |
| Faith Alive Church Ltd | Other services | 2018 |
| Faithjoy Group Ltd | Construction | 2026 |