| Road Bees Company Limited | Transport & logistics | 2023 |
| Roadside Revival and Recovery Ltd | Wholesale & retail | 2026 |
| ROB Johnson Consulting Ltd | Professional & scientific | 2019 |
| Rob’S Roast Ltd | Wholesale & retail | 2025 |
| Rock on Tommy B Ltd | Transport & logistics | 2022 |
| Rockerdar Canine Ltd | Agriculture & fishing | 2023 |
| Rocket's Rentals Ltd | Real estate | 2022 |
| Rockin' Hen's Teeth Ltd | Wholesale & retail | 2019 |
| Rockington & Co Ltd | Real estate | 2022 |
| Roform Steel Decking UK Limited | Transport & logistics | 2015 |
| Roform Steel Fabrications Limited | Manufacturing | 2014 |
| RON Bestwick Limited | Construction | 2016 |
| Rose Cottage Duffield Ltd | Real estate | 2019 |
| Rose Deli Limited | Hospitality | 2023 |
| ROY Jervis and Co Limited | Wholesale & retail | 1975 |
| ROY Nadin Print Limited | Manufacturing | 2012 |
| RRP Slasher Ltd | Wholesale & retail | 2024 |
| Rubicon (UK) Limited | Education | 2011 |
| Ruby Psychological Services Limited | Health & social care | 2015 |
| Rutland House Derbyshire Ltd | Real estate | 2024 |
| Ryan Brothers Limited | Administrative & support | 2008 |
| Ryans Property Management Limited | Real estate | 2014 |
| Ryefield Garages Limited | Real estate | 2023 |
| Ryefield Holdings Limited | Real estate | 2023 |
| Ryefields Close Management Company Limited | Extraterritorial | 2017 |
| Rytech Projects Ltd | Construction | 2022 |
| S & D Property Estates Limited | Real estate | 2024 |
| S & S Shetliffe Limited | Construction | 2004 |
| S + L Components Limited | Manufacturing | 2004 |
| S Burton Block Paving Limited | Construction | 2019 |
| S C Bradshaw Ltd | Professional & scientific | 2017 |
| S J Stainsby Holdings Limited | Arts & recreation | 2018 |
| S L Auto Electrical Limited | Wholesale & retail | 2008 |
| S Lester Public House Ltd | Hospitality | 2024 |
| S P Design (Ripley) Limited | Manufacturing | 2013 |
| S R E Group Limited | Real estate | 2020 |
| S&S Events Limited | Arts & recreation | 2025 |
| S. Chambers Electrical Ltd | Construction | 2016 |
| SAB Training Ltd. | Education | 2014 |
| Sabi House Ltd | Arts & recreation | 2026 |
| Sadler Automotive Limited | Professional & scientific | 2018 |
| Saffiecam Construction Midland Ltd | Construction | 2026 |
| Sage & Plates Catering Ltd | Wholesale & retail | 2021 |
| Saiidy Hasham Ltd | Health & social care | 2024 |
| Sally Cowan Limited | Professional & scientific | 2004 |
| Salt&Co Studio Ltd | Wholesale & retail | 2024 |
| Sam's and Buddy's Limited | Extraterritorial | 2020 |
| Sam's Barbers and Hairdressing Ltd | Other services | 2014 |
| Sammanth'Ears' Limited | Health & social care | 2024 |
| SAP Data Migrators Ltd | Information & communication | 2006 |
| Sarah Draws Ltd | Arts & recreation | 2021 |
| Sarlat Investments Limited | Real estate | 2001 |
| Savage Daughter Ltd | Wholesale & retail | 2020 |
| Saylavy Limited | Professional & scientific | 2014 |
| SB Drainage Limited | Water & waste | 2011 |
| SB Ventilation Limited | Wholesale & retail | 2015 |
| SBS Property & Lettings Ltd | Real estate | 2024 |
| Scaife Heating Ltd | Construction | 2020 |
| Schofield Commercial Interiors Limited | Construction | 2000 |
| School of Theatre Excellence Limited | Education | 2010 |
| SCL Innovation Group Ltd | Manufacturing | 2016 |
| Scott Bathrooms Limited | Construction | 2008 |
| Scott Lorenzo Limited | Administrative & support | 2010 |
| Scott's Garage Limited | Wholesale & retail | 2018 |
| Scpower Limited | Construction | 2026 |
| SDR Derbyshire Ltd | Construction | 2013 |
| Sean Lingwood Ltd | Professional & scientific | 2019 |
| Seat Savants Ltd | Wholesale & retail | 2023 |
| Secretarial Office Services Limited | Extraterritorial | 2001 |
| September Bridal Ltd | Wholesale & retail | 2019 |
| SFT Enterprises Limited | Hospitality | 1999 |
| SG Design and Build (Architectural) Ltd | Construction | 2024 |
| SG Design and Build (Construction) Ltd | Construction | 2023 |
| SGC Properties Limited | Real estate | 2016 |
| SH Rail Engineering Ltd | Professional & scientific | 2020 |
| Sharic Ltd | Transport & logistics | 2018 |
| Sharp Structural Solutions Ltd | Professional & scientific | 2018 |
| Sheerline Bespoke Limited | Manufacturing | 2018 |
| Sheerline Fabrications Limited | Manufacturing | 2019 |
| Sheerline Windows and Doors Limited | Manufacturing | 2019 |
| Sheet Metal Fabrication & Welding Ltd | Manufacturing | 2019 |
| Sherry LEA Limited | Wholesale & retail | 2019 |
| Shine at the Hollies Ltd | Extraterritorial | 2026 |
| Shine Yoga and Pilates Training Ltd | Education | 2024 |
| Shipley Engineering Services Limited | Professional & scientific | 2015 |
| Shire Flooring Limited | Construction | 2016 |
| Shire International Enterprises Limited | Wholesale & retail | 2015 |
| Shottle Hall Developments Limited | Hospitality | 2009 |
| Side Piece Ltd | Hospitality | 2026 |
| Sign and Print (Ripley) Limited | Manufacturing | 2002 |
| Signal Fuels Limited | Transport & logistics | 1999 |
| Sigtech Rail Consultancy Limited | Professional & scientific | 2016 |
| Silicon Forest Ltd | Energy | 2026 |
| Silverback Staffing Limited | Administrative & support | 2018 |
| Silverline GAS, Plumbing & Building Services Ltd | Administrative & support | 2013 |
| Silverwood Rentals Ltd | Real estate | 2021 |
| Simba Systems Ltd | Information & communication | 2018 |
| Simms Home Improvements Ltd | Construction | 2019 |
| Simon Edmonds Limited | Arts & recreation | 2022 |
| Sino Pangaea Trading Ltd | Wholesale & retail | 2007 |