| SJM Interiors (Midlands) Limited | Construction | 2012 |
| SK Hospitality Ltd | Hospitality | 2024 |
| Skillington Workshop Limited | Construction | 1999 |
| Skitterbyte Ltd | Information & communication | 2025 |
| Sky High Rides Limited | Arts & recreation | 2013 |
| Skyline Engineering Design Limited | Professional & scientific | 2024 |
| Slate Construction Ltd | Construction | 2026 |
| SLD Engineering Services Ltd | Professional & scientific | 2020 |
| Slenderella Limited | Extraterritorial | 1997 |
| Slenderella Wholesale Limited | Wholesale & retail | 1999 |
| Smart-R Technology Limited | Other services | 2024 |
| Smarter Letts Ltd | Real estate | 2021 |
| Smartmove (Derbyshire) Limited | Real estate | 2004 |
| Smartmove Homes (Derbyshire) Limited | Real estate | 2013 |
| Smartmove Mortgages Limited | Financial services | 2026 |
| Smiths Caravan Services Limited | Other services | 2017 |
| SMK Property Group Limited | Real estate | 2024 |
| Snapper Media Limited | Professional & scientific | 2022 |
| Snowden Cottages Limited | Real estate | 2010 |
| Snowy 1957 Limited | Extraterritorial | 2013 |
| Soda&Water Limited | Wholesale & retail | 2018 |
| Solit Candles Ltd | Wholesale & retail | 2026 |
| Solo Rose Limited | Professional & scientific | 2016 |
| Solution Spot Company Ltd | Agriculture & fishing | 2025 |
| Sonarose Limited | Real estate | 1974 |
| Soncell Middle East Limited | Administrative & support | 2010 |
| Sonic Communications (International) Limited | Administrative & support | 1976 |
| Sound Dynamics Limited | Wholesale & retail | 1988 |
| Sourceone HR Ltd | Administrative & support | 2019 |
| Southfield Services Limited | Administrative & support | 2012 |
| Southwest Paddlesports Ltd | Education | 2017 |
| Sovereign Peveril Brookfield Limited | Construction | 2019 |
| Spar Property Limited | Construction | 2018 |
| Sparkle and Shine Tutoring Ltd | Education | 2025 |
| SPC Consultancy Ltd | Health & social care | 2025 |
| Spire Plastics Limited | Wholesale & retail | 2012 |
| Spires Homes Limited | Construction | 2005 |
| Splash Zone Ltd | Arts & recreation | 2025 |
| Spondon Carpets and Flooring Limited | Extraterritorial | 2006 |
| Spondon Carpets Limited | Wholesale & retail | 1998 |
| Spondon Property Care.Com Ltd | Extraterritorial | 2010 |
| Spondon Property Holdings Ltd | Real estate | 2021 |
| Springfield Farm (Ambergate) Limited | Wholesale & retail | 2002 |
| Spudwop Ltd | Other services | 2023 |
| SPW Construction Limited | Construction | 2019 |
| Squarise Design Limited | Professional & scientific | 1997 |
| SRB Inns Limited | Hospitality | 2015 |
| SRF Investments Limited | Extraterritorial | 2015 |
| ST Faith's Community Limited | Extraterritorial | 2017 |
| ST Laurence Convent Management Limited | Households | 2004 |
| Stacey FOO Consulting Limited | Information & communication | 2013 |
| Staffordshire Speech Therapy Limited | Health & social care | 2024 |
| Stancliffe Stone Company Limited | Mining & quarrying | 2023 |
| Starch Nemesis Ltd | Hospitality | 2025 |
| Starling Dodd Properties LLP | | 2019 |
| Stately Brides Ltd | Administrative & support | 2009 |
| Station Meadow Management Company Limited | Households | 2013 |
| Steel City Aviation and Training Ltd | Professional & scientific | 2020 |
| Steelytex Limited | Wholesale & retail | 2001 |
| Stepping Stones (Derby) Limited | Real estate | 2015 |
| Steve Barratt Building Solutions Limited | Construction | 2023 |
| Steve Carlier Consulting Ltd | Professional & scientific | 2026 |
| Steve Hulme Plumbing and Heating Ltd | Construction | 2020 |
| Steven Alan Wilson Ltd | Information & communication | 2023 |
| Steven Bradshaw Photography Limited | Professional & scientific | 2009 |
| Steven Pountain Limited | Construction | 2016 |
| Stirling James Risk Management Consultants Ltd | Real estate | 2019 |
| Stix Drum and Percussion Tuition Limited | Education | 2016 |
| STK Electrical (Belper) Ltd | Construction | 2014 |
| Stoneycliff Farms Limited | Agriculture & fishing | 1995 |
| Stourbridge 1967 Limited | Extraterritorial | 2013 |
| Strategy and Beyond Limited | Professional & scientific | 2018 |
| Straw and Jones Limited | Construction | 2020 |
| Street Media Derby Limited | Arts & recreation | 2014 |
| Street Sports Academy Ltd. | Arts & recreation | 2017 |
| Strengthen My Spine Limited | Health & social care | 2018 |
| Strut Style Ltd | Wholesale & retail | 2024 |
| Strutts Centre Limited | Real estate | 2009 |
| STU Walsh Ltd | Information & communication | 2024 |
| Studio 1825 Ltd | Professional & scientific | 2019 |
| Style Barbers Belper Limited | Other services | 2023 |
| Stylistic Hosting Limited | Information & communication | 2021 |
| Stynys Transport Limited | Other services | 2011 |
| Suba Property Ltd | Real estate | 2020 |
| Subism Limited | Information & communication | 2012 |
| Summerside Computers Limited | Information & communication | 2003 |
| Sunleap Limited | Administrative & support | 2020 |
| Sunshine Forest School and Training Centre Ltd | Education | 2026 |
| Susstax Limited | Information & communication | 2023 |
| Sutherland-Hullbailey Blends Ltd | Hospitality | 2021 |
| Suzie Rees Fundraising Ltd | Professional & scientific | 2022 |
| SW Installations (Derbyshire) Ltd | Construction | 2025 |
| Swadlincote Fabrication Ltd | Manufacturing | 2022 |
| Swale Trading Company Limited | Real estate | 2012 |
| Swan Management Services Limited | Information & communication | 2012 |
| Swan Multimedia Limited | Information & communication | 1996 |
| Swan Vehicle Sales Ltd | Wholesale & retail | 2025 |
| Swangrounds Limited | Administrative & support | 2025 |
| SWD Digital Limited | Information & communication | 2009 |
| Swift Staffing Ltd | Administrative & support | 2023 |