| Twentyone (AYR) Ltd | Other services | 2023 |
| TWH Scotland Limited | Information & communication | 2019 |
| Twist Business LP | | 2017 |
| Twohatsdigital.Com Limited | Professional & scientific | 2015 |
| Tygan's Musical Moments Limited | Arts & recreation | 2023 |
| Tympanic EAR Care Ltd | Health & social care | 2024 |
| Ucoz Limited | Real estate | 2016 |
| Uddingston 5 Limited | Hospitality | 2022 |
| UK Clinics Edinburgh Limited | Health & social care | 2015 |
| UK Turf Ltd | Wholesale & retail | 2013 |
| Ultima Building Solutions Ltd | Construction | 2023 |
| Ultimate Leisure (Scotland) Limited | Hospitality | 1973 |
| Ultimate Make-Up and Lashes Limited | Other services | 2012 |
| Uncle Roy's Limited | Manufacturing | 2011 |
| Undersky Ltd | Wholesale & retail | 2024 |
| Underwater EYE Limited | Professional & scientific | 2022 |
| Unforgettable Jewellery Limited | Wholesale & retail | 2013 |
| Universal TDI LP | | 2015 |
| Unlimited Landscapes Construction Ltd | Administrative & support | 2018 |
| Upscale Property Ltd | Construction | 2025 |
| Urban Kandi Limited | Wholesale & retail | 2005 |
| Urium Ltd | Information & communication | 2024 |
| USA Nail Bar 54 Ltd | Other services | 2022 |
| Usługi Prowadzenia Pojazdów Monika Hyska Ltd | Transport & logistics | 2025 |
| Utilityco Ltd | Administrative & support | 2025 |
| Uzair North 1 Ltd | Wholesale & retail | 2018 |
| V G Travel Ltd. | Administrative & support | 2011 |
| V R Growth Limited | Education | 2011 |
| V12 Auto Care Ltd | Arts & recreation | 2022 |
| Vaas-X Ltd | Information & communication | 2025 |
| Vanell Ltd | Hospitality | 2019 |
| Vanilla Joe's Limited | Extraterritorial | 2015 |
| Vanoise Limited | Real estate | 2018 |
| Vansel Trade LP | | 2015 |
| Vaovao Ltd | Information & communication | 2020 |
| Varnylord LP | | 2014 |
| Vast Quantum Ltd | Professional & scientific | 2017 |
| Vavasour Property (Ayrshire) Ltd | Real estate | 2023 |
| Vegos Corp. LP | | 2014 |
| Vekont Trade LP | | 2014 |
| Velanya Ltd | Manufacturing | 2025 |
| Venetian Plaster Specialists Ltd | Construction | 2021 |
| Vennal Cameras Limited | Wholesale & retail | 2003 |
| Venture Lettings Ltd | Real estate | 2019 |
| VENUE38 AYR Ltd | Arts & recreation | 2025 |
| VENUE38 CIC | Arts & recreation | 2025 |
| Vespa Films Limited | Information & communication | 2017 |
| Vest Life Sciences Consulting Limited | Professional & scientific | 2025 |
| Vesta Consult LP | | 2014 |
| Viga Homes (Dalmellington) Limited | Construction | 2013 |
| Viggen Aviation Limited | Transport & logistics | 2023 |
| Villaris Corp. LP | | 2014 |
| Viloso Limited | Information & communication | 2024 |
| Vintage Luxury Chauffeurs Ltd | Transport & logistics | 2022 |
| Virtual Production Hub Ltd | Information & communication | 2025 |
| Virtual Sauce Studios Limited | Information & communication | 2026 |
| Vista Corp. LP | | 2014 |
| Visuplan Limited | Professional & scientific | 2012 |
| Vitality Bathrooms Ltd | Other services | 2024 |
| VL Chauffeurs Ltd | Transport & logistics | 2024 |
| VM Surgical Care Limited | Health & social care | 2025 |
| Voluntary Action South Ayrshire | Other services | 2003 |
| Vonair Aviation Services Limited | Manufacturing | 2015 |
| Vouterie Limited | Wholesale & retail | 2023 |
| Voyage Business Services Ltd | Professional & scientific | 2023 |
| VP Consultancy Services Ltd | Professional & scientific | 2017 |
| VR Shack Ltd | Hospitality | 2025 |
| VTB East LP | | 2014 |
| Vurce Ltd | Administrative & support | 2025 |
| Vycon Products Limited | Administrative & support | 1997 |
| W G Mcharg & Co Ltd. | Professional & scientific | 2011 |
| W S Data Ltd | Information & communication | 2016 |
| W.R Joinery Ltd | Construction | 2022 |
| Waddell Property Group (Residential) Ltd | Real estate | 2025 |
| Waddell Property Group Ltd | Real estate | 2024 |
| Waggon Properties Limited | Real estate | 2016 |
| Walker Commercial Consultancy Ltd | Professional & scientific | 2022 |
| Walker Moore Limited | Real estate | 2013 |
| Walker Realty Limited | Wholesale & retail | 2004 |
| Walker X Ltd | Real estate | 2020 |
| Walker X TD Ltd | Financial services | 2018 |
| Wallace Hodge & Co Limited | Professional & scientific | 2011 |
| Wallacetown Community Energy Project Limited | Energy | 2025 |
| Wallflowers AYR Limited | Wholesale & retail | 2024 |
| Wardblawg Limited | Information & communication | 2010 |
| Warwood Property Investments Ltd | Construction | 2025 |
| Waterfront Entertainment Group Ltd | Arts & recreation | 2026 |
| Waterfront Restaurant @Coyle Ltd | Hospitality | 2020 |
| WAX With Love Ltd | Wholesale & retail | 2026 |
| Waymarker Muse Ltd | Professional & scientific | 2025 |
| WCA Electrical Ltd | Construction | 2016 |
| WD Wealth Management (Holdings) Ltd | Financial services | 2018 |
| WD Wealth Management Ltd | Financial services | 2015 |
| WE ARE Living LAB Ltd | Professional & scientific | 2025 |
| Webb Finance Ltd | Financial services | 2021 |
| Wecan Theatrical Productions Ltd | Arts & recreation | 2025 |
| WEE Knead Desserts LLP | | 2021 |
| WEE Trade Local Ltd | Professional & scientific | 2018 |
| Weemedia Ltd | Information & communication | 2021 |
| Weir Agrishire Services Ltd | Manufacturing | 2023 |