| Weir Wildlife Management Ltd | Agriculture & fishing | 2023 |
| Wellbeing Studios Ltd | Arts & recreation | 2025 |
| Wellington Capital Limited | Real estate | 2022 |
| Wellington House Events Limited | Real estate | 2025 |
| Wellington House Holdings Limited | Real estate | 2025 |
| Wellington School (AYR) Limited | Education | 1948 |
| Wellpark Engineering Ltd | Professional & scientific | 2021 |
| Wellpark Properties Ltd | Real estate | 2020 |
| Wescott Scaffolding Limited | Construction | 2016 |
| West Coast Classic and Car Club Ltd | Other services | 2024 |
| West Coast Classic Car Club Ltd | Other services | 2021 |
| West Coast Classic Car Storage Ltd | Transport & logistics | 2021 |
| West Coast Ices Limited | Hospitality | 2026 |
| West Coast Property Specialists Ltd | Construction | 2025 |
| West Coast Resources Ayrshire Ltd | Administrative & support | 2025 |
| West Coast Venues Group Ltd | Hospitality | 2026 |
| West END Garden Darvel Limited | Real estate | 2025 |
| West Mains Real Estate Investments Limited | Real estate | 2013 |
| West of Scotland Assets Limited | Real estate | 1944 |
| West of Scotland Insurance Brokers (Commercial) Limited | Financial services | 1983 |
| Westclub Limited | Arts & recreation | 2002 |
| Westgale Limited | Construction | 2005 |
| Weston Energy Services Limited | Construction | 2025 |
| What the PUP (Ayrshire) Ltd | Wholesale & retail | 2024 |
| Wheatpark Holdings Limited | Extraterritorial | 2022 |
| Wheatpark Motors Ltd | Extraterritorial | 2022 |
| Whighams Young & Saunders Limited | Real estate | 1968 |
| Whimlers Ltd | Wholesale & retail | 2026 |
| White Cart Chippy Limited | Hospitality | 2014 |
| White House Weddings & Events Ltd | Other services | 2018 |
| White Rose GMT Limited | Construction | 2025 |
| Whitehill Stags Limited | Hospitality | 2023 |
| Whitfield Training Ltd | Education | 2019 |
| Whyte's Carriage Masters Ltd | Administrative & support | 2018 |
| Whyte's Family Funeral Directors Ltd | Other services | 2018 |
| Wilkie Management Limited | Real estate | 1982 |
| Willabert Solutions LP | | 2013 |
| William Duncan + Co (Audit) Ltd | Professional & scientific | 2022 |
| William Duncan + Co (Group) Ltd | Financial services | 2021 |
| William Duncan + Co Ltd | Professional & scientific | 2013 |
| Willie Wastles Ltd | Hospitality | 2022 |
| Willis Clinics Ltd | Health & social care | 2009 |
| Willjam Properties Limited | Real estate | 2020 |
| Wills Enterprises Limited | Hospitality | 2023 |
| Wilson Forest Products Limited | Agriculture & fishing | 2002 |
| Wilson Imports Limited | Wholesale & retail | 1994 |
| Wilson Renewables II LLP | | 2015 |
| Wilson Renewables III LLP | | 2022 |
| Wilson Renewables IV LLP | | 2022 |
| Windrose Removals Ltd | Transport & logistics | 2026 |
| Wireless Telecoms Services Limited | Information & communication | 2008 |
| Wisdom Education Academy Ltd | Education | 2020 |
| Witch Limited | Information & communication | 2007 |
| WM Cumming & SON (AYR) Ltd | Construction | 2021 |
| WMA Group Limited | Real estate | 2011 |
| Wnrgp Limited | Administrative & support | 2015 |
| Wood Graham Property Investments Ltd | Real estate | 2019 |
| Wood Outdoor Limited | Professional & scientific | 2013 |
| Wood Property Group (Lorne Arcade) Limited | Construction | 2023 |
| Wood Property Group Ltd | Construction | 2019 |
| Woodfield Stores Local Limited | Wholesale & retail | 2023 |
| Woodlands Cars Ayrshire Ltd | Transport & logistics | 2024 |
| Woodpark Stores Limited | Wholesale & retail | 2014 |
| Woodstone Insulation Ltd | Professional & scientific | 2026 |
| Woodwynd Limited | Professional & scientific | 2023 |
| WRE Consultants Ltd | Professional & scientific | 2024 |
| Wright Roofing Services Ltd | Construction | 2025 |
| WS Facilities Ltd | Wholesale & retail | 2025 |
| Wthr Ltd | Information & communication | 2025 |
| WU11SON Waste Management Ltd | Water & waste | 2020 |
| Wulyum's Ltd | Hospitality | 2022 |
| WXS Consulting (UK) Limited | Information & communication | 2018 |
| Wyliefoxmedia Ltd | Professional & scientific | 2024 |
| XPC International Limited | Professional & scientific | 2005 |
| Xpert Properties & Investment Ltd | Real estate | 2025 |
| Xtra Accounting Ltd | Professional & scientific | 2013 |
| Xtreme Fit Lanark Ltd | Other services | 2023 |
| XU AYR Ltd | Hospitality | 2025 |
| Yarra Investments Limited | Administrative & support | 2010 |
| Yelsel Limited | Wholesale & retail | 2025 |
| Yournest Limited | Other services | 2016 |
| Yuen Aviation Limited | Manufacturing | 2023 |
| Yuille Holdings Ltd | Financial services | 2023 |
| Zaynax Limited | Wholesale & retail | 2024 |
| Zeen Properties Ltd | Construction | 2015 |
| ZEN Business Ltd | Professional & scientific | 2025 |
| ZEN Commercial Finance Limited | Financial services | 2016 |
| Zengus Ventures Ltd | Professional & scientific | 2024 |
| Zmat Ltd | Hospitality | 2016 |
| Zotax Foods Ltd | Wholesale & retail | 2025 |
| ZPF Aero Structures Ltd | Professional & scientific | 2025 |
| ZPM Services Ltd | Professional & scientific | 2026 |
| Zylovista Marketing Ltd | Professional & scientific | 2024 |