| THE Little Art School Company Limited | Education | 2014 |
| THE Little Art School Franchise Ltd | Education | 2022 |
| THE Little Art School Glasgow Limited | Education | 2016 |
| THE Little Art School Trading Company Ltd | Education | 2020 |
| THE Lucky Star Scotland Ltd | Hospitality | 2020 |
| THE Mckinstry Company LLP | | 2016 |
| THE Meadows Private Day Nursery Ltd | Health & social care | 2016 |
| THE Mill Cafe Ltd | Hospitality | 2024 |
| THE Music Spot (Ayrshire) Ltd | Education | 2018 |
| THE National Health, Safety & Risk Alliance Limited | Hospitality | 2025 |
| THE New Clansman Ltd. | Hospitality | 2018 |
| THE North Harbour Cafe Ltd | Hospitality | 2023 |
| THE Pawswell Group Ltd | Other services | 2025 |
| THE Pilates Club Studios Ltd | Other services | 2025 |
| THE PIV Beer Garden Ltd | Hospitality | 2025 |
| THE Rebel Baker Ltd | Manufacturing | 2024 |
| THE Rupee Room Limited | Hospitality | 2010 |
| THE School of Psychology Online Limited | Education | 2015 |
| THE Skin Clinic AYR Ltd | Health & social care | 2024 |
| THE Sleeping OWL Hospitality Ltd | Hospitality | 2019 |
| THE Spray Paint GUY & Co Ltd | Manufacturing | 2021 |
| THE Tipsy Lairds Tours Limited | Administrative & support | 2020 |
| THE Tudor (AYR) Ltd | Other services | 2021 |
| THE Turnstyles Enterprise CIC | Health & social care | 2025 |
| THE Water of AYR and TAM O'Shanter Hone Works Limited | Real estate | 1900 |
| THE WEE Shop (AYR) Limited | Wholesale & retail | 2025 |
| THE Weee Shop Ltd | Wholesale & retail | 2025 |
| THE Zone Initiative Limited | Arts & recreation | 2003 |
| Theatre Online Ltd | Arts & recreation | 2021 |
| Thedesignerrooms.Com Limited | Wholesale & retail | 2021 |
| Therapy and Coaching Services Ltd | Education | 2026 |
| Think Forward Coaching Ltd | Health & social care | 2022 |
| Thinking Forword AYR Limited | Information & communication | 2026 |
| Thinking Forword Social Community Interest Company | Information & communication | 2026 |
| This Must BE the Place Stays Ltd | Real estate | 2022 |
| Thistle & Rose Ltd | Manufacturing | 2015 |
| Thistle B Kilts Limited | Wholesale & retail | 2026 |
| Thistle Education Limited | Professional & scientific | 2012 |
| Thistle Education Solutions Limited | Education | 2012 |
| Thistle Fencing Ltd | Construction | 2022 |
| Thistle Homes Limited | Real estate | 2024 |
| Thistle Joinery (AYR) Ltd | Construction | 2021 |
| Thistle Realty Holdings Limited | Professional & scientific | 2020 |
| Thomson and Gray Limited | Administrative & support | 2000 |
| Thornborrow Farming Ltd | Agriculture & fishing | 2024 |
| Three Anchors Ltd | Hospitality | 2025 |
| Three SIX One Properties Ltd | Real estate | 2021 |
| Thrive Care Scotland Limited | Health & social care | 2024 |
| Tidal Electric UK Ltd | Energy | 2026 |
| Tietoisuusakatemia Ltd | Education | 2026 |
| Tikl Solutions LP | | 2017 |
| TIN Rocket Space Limited | Professional & scientific | 2022 |
| Tioram Ltd | Administrative & support | 2021 |
| Titchfield Traders Ltd | Wholesale & retail | 2024 |
| TJM Sales Solutions Ltd | Professional & scientific | 2023 |
| TK Automotive Limited | Wholesale & retail | 2013 |
| Tm&H Recruitment Ltd | Administrative & support | 2023 |
| TM@NO27 Ltd | Other services | 2022 |
| TMS Plumbing and Heating Ltd | Construction | 2024 |
| Toner & Mccartney LLP | | 2012 |
| Tool-Col Ltd | Wholesale & retail | 2021 |
| Toor Superstore Ltd | Wholesale & retail | 2013 |
| Top Cat Blinds Ltd | Construction | 2020 |
| Top Tier Scaffolding Solutions Ltd | Construction | 2022 |
| Topcom Development LP | | 2014 |
| Torbit Engineering Ltd | Manufacturing | 2017 |
| Tormore Properties Limited | Real estate | 2021 |
| Toro Blanco Personal Training Ltd | Other services | 2019 |
| Total Global Solutions Ltd | Professional & scientific | 2026 |
| Total Turfing Holdings Ltd | Administrative & support | 2025 |
| TPS1097 Ltd | Professional & scientific | 2023 |
| TR Group (Scot) Ltd | Hospitality | 2019 |
| Tractor Exports (Heavy Haulage) Ltd | Transport & logistics | 2017 |
| Tractor Exports Limited | Wholesale & retail | 2004 |
| Tracy Taylor Hairdressing Limited | Other services | 2004 |
| Trade and Union (UK) Limited | Professional & scientific | 2023 |
| Trade Today Ltd | Wholesale & retail | 2013 |
| Tradebeaver Ltd | Extraterritorial | 2018 |
| Tradition Giftware Limited | Wholesale & retail | 2010 |
| Trainlikeapro AYR Limited | Arts & recreation | 2024 |
| Tralorg Benefits Limited | Other services | 2018 |
| Trans Forwarding LP | | 2014 |
| Transcontinental Trade Group LP | | 2017 |
| Traylen Estates Limited | Agriculture & fishing | 2025 |
| Tree Business Ayrshire Ltd | Administrative & support | 2024 |
| Treehouse Carpentry Ltd | Construction | 2020 |
| Treehouse Property Scotland Ltd | Real estate | 2026 |
| Trend Textile Ltd | Wholesale & retail | 2020 |
| Trfk Limited | Professional & scientific | 2023 |
| Trianex LP | | 2014 |
| Trismega LP | | 2014 |
| Trosten Limited | Real estate | 2009 |
| Truesdale Property Company Limited | Administrative & support | 1998 |
| Trysting Thorn Hotel Ltd | Hospitality | 2015 |
| Tsmm Marketing Ltd | Information & communication | 2024 |
| Tsukure Hub CIC | Hospitality | 2019 |
| Tu-Max Storage Limited | Transport & logistics | 2014 |
| Tuatha Trails Ltd | Manufacturing | 2016 |
| TWA Dugs Design Ltd | Professional & scientific | 2020 |
| Twenty-Seven Alloway Limited | Wholesale & retail | 2008 |