| Tansa Management LP | | 2014 |
| Tarelgin Butchers Limited | Wholesale & retail | 2014 |
| Target Mot's Ltd | Wholesale & retail | 2017 |
| Target Renewables Ltd | Professional & scientific | 2013 |
| Tartan DNA Limited | Extraterritorial | 2019 |
| Tartanice Ltd | Manufacturing | 2015 |
| Tasty's Ayrshire Ltd | Hospitality | 2022 |
| Tax.Scot Limited | Professional & scientific | 2022 |
| Taylor & Reilly Home Improvements Ltd | Construction | 2025 |
| Taylor Industrial Supplies Ltd | Wholesale & retail | 2010 |
| Taylor Private Clients Ltd | Financial services | 2026 |
| Taylor Welding & Fabrication Ltd | Manufacturing | 2022 |
| Taylor's Entertainments Limited | Arts & recreation | 1973 |
| Taylormarine Support Ltd | Manufacturing | 2019 |
| TAZ Tandoori Limited | Hospitality | 2016 |
| Taza AYR ZP Ltd | Hospitality | 2022 |
| TB Carpets Ltd | Wholesale & retail | 2026 |
| Tbayla Ltd | Wholesale & retail | 2025 |
| TBC Renewables Limited | Professional & scientific | 2024 |
| Tcgblox Ltd | Wholesale & retail | 2025 |
| TDR Braehead Ltd | Real estate | 2019 |
| TDR Furniture Ltd | Real estate | 2020 |
| TDR Group Holdings Ltd | Real estate | 2020 |
| TDR Uddingston Ltd | Real estate | 2022 |
| TDR Warehouse Ltd | Real estate | 2020 |
| Team Barbers (AYR) Ltd | Other services | 2023 |
| TEC40 Ltd | Professional & scientific | 2022 |
| Techpoint Glasgow Ltd | Wholesale & retail | 2019 |
| Tectorum Property Ltd | Construction | 2011 |
| TED Nails Ayrshire Ltd | Other services | 2026 |
| Teebone Hair Ltd | Other services | 2023 |
| TEK Box (AYR) Ltd | Administrative & support | 2021 |
| Tektup Limited | Information & communication | 2015 |
| Temperature Control Services Limited | Professional & scientific | 2025 |
| Temple House Care Home Limited | Health & social care | 1997 |
| Templedouglas Ltd | Real estate | 2024 |
| Tenny Nursery Ltd | Health & social care | 2025 |
| TES Solutions Ltd | Wholesale & retail | 2026 |
| Test My Electrics Limited | Construction | 2020 |
| Tetris Promotions Ltd | Arts & recreation | 2017 |
| TFL Aerotech Ltd | Manufacturing | 2022 |
| TG Scaffolding (AYR) Ltd | Construction | 2025 |
| TH Nails Studio Ltd | Other services | 2026 |
| Thai Phanya Limited | Hospitality | 2015 |
| THE Airshow (Scotland) Ltd. | Arts & recreation | 2014 |
| THE Allisons Cafe Limited | Hospitality | 2020 |
| THE Anchor Bar (AYR) LLP | | 2020 |
| THE Arthurlie Inns Ltd | Hospitality | 2011 |
| THE Avenue Laundrette Ltd | Other services | 2024 |
| THE Avon Valley Housing Association Ltd | Administrative & support | 2013 |
| THE AYR United Football and Athletic Club, Limited | Arts & recreation | 1905 |
| THE Ayrshire Hospice | Health & social care | 1987 |
| THE Backstreet Social Ltd | Hospitality | 2026 |
| THE Beauty Spot Spa (Glasgow) Ltd | Other services | 2025 |
| THE BED Room AYR Ltd | Wholesale & retail | 2006 |
| THE BEE Hive Hotel Ltd | Hospitality | 2024 |
| THE Bellrock Bar Ltd | Hospitality | 2025 |
| THE Bettii POD Ltd | Wholesale & retail | 2021 |
| THE Bills Have Y'S Limited | Information & communication | 2021 |
| THE Blankfaces CIC | Wholesale & retail | 2018 |
| THE Boolers Limited | Hospitality | 2023 |
| THE Bullish Coffee Company Ltd | Wholesale & retail | 2022 |
| THE Business Timesaver Ltd | Administrative & support | 2013 |
| THE Centre for Integrative Nutrition Ltd | Education | 2019 |
| THE Centre for Integrative Sports Nutrition Ltd | Education | 2017 |
| THE Cleaning Fairies Ayrshire Ltd | Administrative & support | 2026 |
| THE Coast Clinic Limited | Other services | 2011 |
| THE Commercial Photographers Network Limited | Professional & scientific | 2014 |
| THE COO Shed Limited | Wholesale & retail | 2020 |
| THE Crafty COO Ltd | Wholesale & retail | 2024 |
| THE Dark Sky Observatory | Education | 2011 |
| THE Deli Troon Limited | Other services | 2011 |
| THE Democracy Project CIC | Education | 2026 |
| THE Dental Clinic AYR Ltd | Health & social care | 2025 |
| THE Dogs Deli Scotland Ltd | Wholesale & retail | 2024 |
| THE Drongan Fish and Chips Limited | Hospitality | 2026 |
| THE Emma Beauty Studio Ltd. | Other services | 2020 |
| THE Event Company (GB) Limited | Extraterritorial | 2017 |
| THE FAT Pizza AYR Ltd | Hospitality | 2024 |
| THE Fluxxworks Limited | Professional & scientific | 2017 |
| THE Foxybadger Limited | Education | 2012 |
| THE Free Range Dairy Company (Scotland) Limited | Extraterritorial | 2016 |
| THE Garden Room Coffee Ltd | Hospitality | 2026 |
| THE Ghillie DHU Limited | Hospitality | 2019 |
| THE Glencara Estate Company | Real estate | 1925 |
| THE Greenside Restaurant Ltd | Hospitality | 2020 |
| THE Groomery Ltd | Other services | 2022 |
| THE Harbourside Coffee and Kitchen Ltd | Hospitality | 2026 |
| THE Harvest Bar 2020 Ltd | Hospitality | 2020 |
| THE Hatinator Limited | Wholesale & retail | 2026 |
| THE Honeybee and the Hare Ltd | Hospitality | 2024 |
| THE House of Alicia Ltd | Hospitality | 2024 |
| THE Iris Arts AYR CIC | Administrative & support | 2019 |
| THE Irvine Diet Centre Ltd | Professional & scientific | 2021 |
| THE Isles Workshop LLP | | 2022 |
| THE Kilted Kitchen Limited | Wholesale & retail | 2021 |
| THE Kyle Rise Community Car Park CIC | Transport & logistics | 2026 |
| THE Kyle Rise Ltd | Other services | 2025 |
| THE Last Minute Barmaid Ltd | Hospitality | 2023 |
| THE Little Art School Ayrshire Limited | Education | 2019 |