| RSM Inns Ltd | Hospitality | 2024 |
| RT Civils and Groundworks Ltd | Construction | 2021 |
| Ruby Ysabelle Hair Limited | Other services | 2018 |
| Rubys-Gifts Ltd | Wholesale & retail | 2024 |
| Rural Business Academy Limited | Information & communication | 2025 |
| Ruralink Ltd | Administrative & support | 2026 |
| RV SUB Ltd | Wholesale & retail | 2025 |
| RW British Certification Limited | Professional & scientific | 2023 |
| Rward Advisory Ltd | Information & communication | 2026 |
| RWM 2007 Limited | Financial services | 2024 |
| Ryan Yeomans Limited | Wholesale & retail | 2026 |
| S F Dale Commercials Limited | Extraterritorial | 2016 |
| S F Dale Limited | Transport & logistics | 2002 |
| S Farmer Consulting Limited | Administrative & support | 2010 |
| S Frith Limited | Agriculture & fishing | 2012 |
| S T Allen and SON Limited | Agriculture & fishing | 2012 |
| S Wood Electrical Contractors Ltd | Construction | 2020 |
| Sackville Developments (Warwick) Limited | Real estate | 2005 |
| Sacs of Ashbourne Limited | Wholesale & retail | 2021 |
| Safetytech Limited | Other services | 2021 |
| Salcombe Court Management Development Company Limited | Real estate | 2006 |
| Sallyfield Lane Limited | Hospitality | 2019 |
| Salt Consultants and Property Investments Ltd | Real estate | 2018 |
| Saracens Court Management Company Limited | Households | 2010 |
| Sarah Stanley-Smith Limited | Professional & scientific | 2017 |
| Saul Court Management Limited | Households | 2003 |
| Savonches Limited | Wholesale & retail | 2025 |
| SB & CL Properties Limited | Real estate | 2016 |
| Scarlett Security Solutions Ltd | Administrative & support | 2013 |
| Schneck Consulting Limited | Professional & scientific | 2021 |
| Schule Developments Ltd | Real estate | 2018 |
| Scope Automotive Limited | Wholesale & retail | 2016 |
| Scope Vehicle Care Limited | Extraterritorial | 2025 |
| Scott and Barlow Industrial Control Systems Ltd | Manufacturing | 2025 |
| SCR Contractors Ltd | Construction | 2024 |
| Screen Services Engineering Limited | Construction | 2006 |
| SDS Metal Decking Limited | Construction | 2017 |
| Sea Your Dreams Limited | Professional & scientific | 2005 |
| Seals Investments | Administrative & support | 2019 |
| Sean Clayton Limited | Wholesale & retail | 2025 |
| Searle Sports UK Limited | Arts & recreation | 2012 |
| Seasonal Productions Ltd | Arts & recreation | 2024 |
| Second Chance Dress Agency Limited | Wholesale & retail | 1999 |
| Secor Consultants Ltd | Professional & scientific | 2008 |
| Secure Base Psychological Services Ltd | Health & social care | 2017 |
| Securecom Limited | Real estate | 1997 |
| Segreto Ltd | Hospitality | 2019 |
| Self Storage Lichfield Limited | Other services | 2018 |
| Sellers Asset Finance Limited | Professional & scientific | 2024 |
| Sellers Building Services Ltd | Construction | 2021 |
| Sellers Developments Limited | Construction | 2016 |
| Sellors Fine Jewellery Limited | Manufacturing | 2026 |
| Sennon Limited | Information & communication | 2004 |
| Sentry Innovations Limited | Professional & scientific | 2019 |
| Servants of the Light Ltd | Other services | 2017 |
| SES Electronic Solutions Ltd | Administrative & support | 2025 |
| SH Driving Solutions Ltd | Administrative & support | 2012 |
| Shaws Equestrian Limited | Wholesale & retail | 2013 |
| Shaws Iberian Limited | Wholesale & retail | 2021 |
| Shaws Properties Limited | Real estate | 2008 |
| SHE Business Solutions Ltd | Administrative & support | 2013 |
| Sheard of Uttoxeter Limited | Real estate | 1970 |
| Shearer Holdings (SJ) Limited | Real estate | 2022 |
| Shearstud Supplies Ltd | Manufacturing | 2018 |
| Shentons Garage Limited | Wholesale & retail | 2009 |
| Shhs Solutions Ltd | Construction | 2025 |
| Shirleymill Limited | Transport & logistics | 2018 |
| Signatrace Ltd | Information & communication | 2026 |
| Signia Park Management Company Limited | Real estate | 2021 |
| Sillettz Ltd | Wholesale & retail | 2025 |
| Sills Farming Limited | Agriculture & fishing | 2020 |
| Simon AS a Service Limited | Information & communication | 2026 |
| Simon Himsworth Associates Limited | Professional & scientific | 2008 |
| Simply the Best Bags Limited | Wholesale & retail | 2022 |
| Simranger Limited | Manufacturing | 2013 |
| Sindenland Limited | Hospitality | 1990 |
| SIP Coffee and Wine Limited | Hospitality | 2025 |
| SJH Lean Consulting Ltd | Professional & scientific | 2024 |
| Skeet Brothers Limited | Real estate | 1954 |
| Skip and Scramble Limited | Professional & scientific | 2024 |
| SM Hair Salons Limited | Other services | 2003 |
| Small Oaks Childcare Limited | Education | 2017 |
| Small Wonders Clothing Ltd | Wholesale & retail | 2024 |
| Smithy Fields Campsite Ltd | Hospitality | 2017 |
| SMQ Media PR & Services Limited | Manufacturing | 2012 |
| Sniff and Snack Ltd | Manufacturing | 2025 |
| Sonyl Consulting Ltd | Information & communication | 2020 |
| South Dales Healthcare Ltd | Health & social care | 2021 |
| South Street ASH Ltd | Hospitality | 2024 |
| Spar Shop (Repton) Limited | Wholesale & retail | 1998 |
| Specialist Advisors Limited | Financial services | 2014 |
| SPORT4REAL Ltd | Administrative & support | 2012 |
| Sportive Cyclist Ltd | Information & communication | 2016 |
| Spryly Ltd | Professional & scientific | 2024 |
| Squeeze PIN Limited | Administrative & support | 2017 |
| SRB Accountancy Limited | Professional & scientific | 2018 |
| ST Webster Joinery & Building Services Ltd | Construction | 2019 |
| Stadium Retail Park Management Company Limited | Real estate | 2024 |
| Staffordshire Bespoke Joinery Limited | Construction | 2009 |
| Staffordshire Care Farming Development C.I.C. | Education | 2010 |