| Stand Properties Ltd | Real estate | 2021 |
| Standard Hound Ltd | Hospitality | 2024 |
| Star Aviation Fluid Services Ltd | Transport & logistics | 2021 |
| Stay Derbyshire Ltd | Real estate | 2022 |
| Steadfast UK Fire Safety Ltd | Public administration | 2025 |
| Stellacloud Limited | Information & communication | 2012 |
| Stenic Property Investments Limited | Real estate | 2004 |
| Stepping Stones Shoes Limited | Wholesale & retail | 2012 |
| Steven Holmes Carpets Limited | Wholesale & retail | 2010 |
| Sticky Fingers Trading Limited | Wholesale & retail | 2010 |
| Stoneburton Limited | Real estate | 2020 |
| Stoney & Cross Ltd | Professional & scientific | 2019 |
| Stoney Stanton Business Park Management Company Limited | Real estate | 2020 |
| Strategic Lines Limited | Professional & scientific | 2002 |
| Stuart Green Research Limited | Professional & scientific | 2012 |
| Stuart Sellers Limited | Construction | 2003 |
| Stubwood (UK) Limited | Agriculture & fishing | 2010 |
| Stud Brook Business Park Management Company Limited | Real estate | 2024 |
| Stud-Deck Services Ltd | Construction | 2011 |
| Studio Harrison Ltd | Professional & scientific | 2024 |
| Sturston Developments Limited | Construction | 2016 |
| Sturston Garage Limited | Wholesale & retail | 2002 |
| Stydd House Equestrian Limited | Agriculture & fishing | 2021 |
| Style Annex Limited | Wholesale & retail | 2026 |
| Styrecast Limited | Manufacturing | 2000 |
| SU Casa Conveyancing Ltd | Professional & scientific | 2021 |
| Su'Per Nosh Limited | Hospitality | 2012 |
| Sudbury Farms Limited | Agriculture & fishing | 2007 |
| Sudbury Gasworks Restoration Trust Ltd | Real estate | 2014 |
| Sun-Days Tanning (Ashbourne) Limited | Other services | 2025 |
| Sunflower Accountancy Limited | Professional & scientific | 2023 |
| Superlytics Ltd | Professional & scientific | 2020 |
| Sutton-On-The-Hill Farms Limited | Agriculture & fishing | 1963 |
| Sweet Vips Ltd | Arts & recreation | 2025 |
| Swinderby (Chaddesden) Management Company Limited | Administrative & support | 1982 |
| Swinscoe Camping Ltd | Hospitality | 2024 |
| Swuk Steel Decking Limited | Construction | 2011 |
| Swuk Steel Decking Supplies Limited | Construction | 2014 |
| Sycamore Farm Properties Limited | Construction | 2020 |
| T & G Stone Ltd | Administrative & support | 2018 |
| T Greatorex Engineering Limited | Construction | 2010 |
| T L Groundworks & Surfacing Limited | Construction | 2021 |
| T Worsey Ltd | Agriculture & fishing | 2024 |
| Talbot Consulting Limited | Other services | 2012 |
| Tamper Technologies Group Limited | Professional & scientific | 2012 |
| Tamper Technologies Limited | Manufacturing | 2002 |
| Tampertape Limited | Professional & scientific | 2009 |
| Tampertech Limited | Professional & scientific | 2009 |
| Tanglewood LLP | | 2015 |
| TAO Adventure Therapies Ltd | Education | 2023 |
| Tayco Ltd | Administrative & support | 2006 |
| Taylor-Smith Solutions Limited | Professional & scientific | 2011 |
| TDB Events Ltd | Hospitality | 2022 |
| TDR Design Services Limited | Professional & scientific | 2015 |
| Teal Laboratory Service Limited | Professional & scientific | 2015 |
| Team Radford Show Horses Ltd | Agriculture & fishing | 2026 |
| Tear UP Company Ltd | Arts & recreation | 2024 |
| Tear UP Festival C.I.C. | Arts & recreation | 2025 |
| Technical Capping Services Limited | Professional & scientific | 2015 |
| Tecinteractive Limited | Information & communication | 2005 |
| Tell the Housekeeper Ltd | Administrative & support | 2024 |
| Terrier Tech Limited | Professional & scientific | 1998 |
| Teveq Ltd | Transport & logistics | 2025 |
| Tgis Aviation Limited | Transport & logistics | 2002 |
| That's the Ticketing Ltd | Information & communication | 2024 |
| That's WHY Limited | Professional & scientific | 2025 |
| THE 137/141 Park Road Buxton Management Company Limited | Households | 2002 |
| THE 3D Print Business Limited | Manufacturing | 2014 |
| THE 3D Service Business Limited | Information & communication | 2014 |
| THE 58 ST John Street Ashbourne Management Company Limited | Households | 2006 |
| THE AI Guys Group Ltd | Information & communication | 2024 |
| THE Artisan Handyman Limited | Construction | 2021 |
| THE Ashbourne Little BUS Company Limited | Transport & logistics | 2014 |
| THE Attachment Consultancy Limited | Education | 2009 |
| THE Best Tapas Ltd | Hospitality | 2025 |
| THE Big PIG PIE Company Ltd | Manufacturing | 2018 |
| THE Birdsgrove Fly-Fishing Club Ltd | Agriculture & fishing | 1992 |
| THE Burden Basket Limited | Health & social care | 2007 |
| THE Carsington Sailing Club Limited | Arts & recreation | 1990 |
| THE Catering Barn Limited | Hospitality | 2025 |
| THE Clayrooms LB Limited | Arts & recreation | 2018 |
| THE Clayrooms Limited | Financial services | 2023 |
| THE Clayroomsfranchise Ltd | Administrative & support | 2023 |
| THE Clayroomsmacc Ltd | Arts & recreation | 2023 |
| THE Co-Working Spot Ltd | Real estate | 2025 |
| THE COW Dalbury Limited | Hospitality | 2014 |
| THE Crown Inn (Marston) Limited | Hospitality | 2015 |
| THE Derwent Water Arms Ltd | Hospitality | 2012 |
| THE Duncombe Arms Limited | Hospitality | 2011 |
| THE Early Talent Consultancy Ltd | Administrative & support | 2020 |
| THE George & Dragon Ashbourne Limited | Hospitality | 2022 |
| THE Heavy Tractor Group 37714 | Other services | 2017 |
| THE Hopton-Wood Stone Firms Limited | Manufacturing | 1998 |
| THE Horse Long Lane Limited | Hospitality | 2014 |
| THE Ilam Cross Trust | Professional & scientific | 2003 |
| THE Kitchen & Bar Co Ltd | Hospitality | 2024 |
| THE Little Shed Annex Limited | Wholesale & retail | 2025 |
| THE Little Shed Bakery Ltd | Hospitality | 2024 |
| THE Little Shed Gift Shop Ltd | Wholesale & retail | 2022 |
| THE Longship Trading Company Ltd. | Education | 1992 |