| EWC4 Ottringham Limited | Energy | 2019 |
| EWH Group Limited | Financial services | 2021 |
| EWH Properties (Burnside) Limited | Real estate | 2020 |
| Exact Start Limited | Wholesale & retail | 2015 |
| Excel Cleaning & Property Maintenance Ltd | Administrative & support | 2022 |
| Excel GAS Services Limited | Construction | 2003 |
| Excel Learning Trust | Education | 2016 |
| Excel Sports Clinic Limited | Health & social care | 2019 |
| Excel Trust Company Limited | Financial services | 1997 |
| Excelgens Consulting UK Limited | Information & communication | 2023 |
| Excellence Floorcare Limited | Administrative & support | 2016 |
| Exchange Properties Limited | Extraterritorial | 1991 |
| Executive Miles Limited | Health & social care | 2026 |
| Exell Technology Limited | Manufacturing | 1995 |
| Exellfoods Ltd | Hospitality | 2023 |
| Exhibition Hotel (York) Limited | Hospitality | 2012 |
| Exhibition Hub UK Ltd | Administrative & support | 2019 |
| Exley Wobbly Club Ltd. | Other services | 2012 |
| Exodus (Yorkshire) Limited | Other services | 2005 |
| Exomens Limited | Information & communication | 2008 |
| Exotic Eco Ltd | Agriculture & fishing | 2025 |
| Expatsinfrance Accountant Limited | Professional & scientific | 2009 |
| Expeditionary Software Company Limited | Information & communication | 2026 |
| Experience Counts (York) CIC | Other services | 2016 |
| Expert Psychological Reports Ltd | Health & social care | 2012 |
| Expert Quantum Reports Ltd | Professional & scientific | 2025 |
| Expert Witness Network Limited | Public administration | 2021 |
| Explore Enterprises Limited | Hospitality | 2015 |
| Explosive Developments Limited | Manufacturing | 1983 |
| Exposure Property Marketing Limited | Professional & scientific | 2009 |
| Express Electronics Limited | Wholesale & retail | 2004 |
| Expression Homes Ltd | Construction | 2014 |
| Expresso Property Limited | Construction | 2014 |
| Exto Consult Ltd | Professional & scientific | 2021 |
| Extra Electrical Ltd | Construction | 2016 |
| Extran Limited | Extraterritorial | 2002 |
| Extratext Limited | Households | 1982 |
| Extreme by Design Limited | Wholesale & retail | 2013 |
| Extreme Yorkshire Power Washing Ltd | Administrative & support | 2024 |
| Extreme-Eyewear Limited | Health & social care | 2007 |
| Exuberant Soul Limited | Hospitality | 2024 |
| EYE Exam (Holdings) Limited | Financial services | 2023 |
| EYE Exam Limited | Information & communication | 2002 |
| EYE of York Apartments Limited | Real estate | 2023 |
| EYE of York Limited | Real estate | 2021 |
| Eyecare Payments Limited | Information & communication | 2007 |
| Eyesthetic Limited | Health & social care | 2010 |
| Ezee Parts Limited | Wholesale & retail | 2021 |
| Ezra Management Services Ltd | Administrative & support | 2016 |
| F & E Developments Ltd | Construction | 2000 |
| F & H Development Limited | Real estate | 2019 |
| F & S Cleaning York Ltd | Administrative & support | 2025 |
| F Atkinson (Potatoes) Limited | Agriculture & fishing | 2022 |
| F Bayram & Sons Limited | Transport & logistics | 1995 |
| F&P (Huddersfield) Limited | Hospitality | 2021 |
| F. Machin & Sons Limited | Agriculture & fishing | 1977 |
| F.C. Spear Limited | Extraterritorial | 1926 |
| F.D. Todd & Sons Limited | Water & waste | 1963 |
| F.W.Wood & Son(York)Limited | Real estate | 1955 |
| F.Y.R. Project Management Services Ltd | Construction | 2003 |
| F10 Construction Co-Ordination Limited | Professional & scientific | 2014 |
| F13 Marketing Ltd | Professional & scientific | 2014 |
| F17 Limited | Professional & scientific | 2015 |
| F4I Ltd | Professional & scientific | 2024 |
| F8 Group Limited | Professional & scientific | 2025 |
| Faber Garage Car Sales Ltd | Wholesale & retail | 2022 |
| Faber Garage Ltd | Wholesale & retail | 2022 |
| Fabler & Co. Ltd | Hospitality | 2020 |
| Fabler Bars Lendal Ltd | Hospitality | 2024 |
| Fabler Bars Ltd | Hospitality | 2022 |
| Fabr Global Limited | Administrative & support | 2025 |
| Fabriceyorkman Ltd | Transport & logistics | 2024 |
| Face 4 Limited | Wholesale & retail | 2017 |
| Facesthetics Limited | Health & social care | 2017 |
| Facetiq Limited | Manufacturing | 2026 |
| Factory Golf Limited | Education | 2025 |
| Faculty of Queen Ethelburga's Limited | Administrative & support | 1996 |
| Fair Deal Lets Limited | Households | 2011 |
| Fair Mile Rise (Blandford ST Mary) Management Company Limited | Real estate | 2018 |
| Fairclough & Partners LLP | | 2024 |
| Fairey Enterprises Limited | Professional & scientific | 2019 |
| Fairfax House York Enterprises Limited | Wholesale & retail | 2002 |
| Fairfield Manor Management Company Limited | Households | 2007 |
| Fairfield Property Holdings Limited | Financial services | 2019 |
| Fairfield Property Limited | Real estate | 2016 |
| Fairfield Property Services (York) Limited | Construction | 2010 |
| Fairford Corby Limited | Professional & scientific | 2014 |
| Fairmoor (Morpeth) Management Company Limited | Households | 2016 |
| FAIRMOUNT88 Ltd | Extraterritorial | 2016 |
| Fairsing Impact Advisory Limited | Professional & scientific | 2017 |
| Fairway Construction (Leeds) Ltd | Construction | 2023 |
| Fairway Sports Limited | Arts & recreation | 2012 |
| Fairways (Retford) Management Company Limited | Households | 2015 |
| Fairytale and Dance Party Events Limited | Administrative & support | 2019 |
| Faiz & Sons York Ltd | Real estate | 2025 |
| FAL Limited | Professional & scientific | 2022 |
| Falcon Pubs (York) Limited | Hospitality | 2026 |
| Falconer Limited | Health & social care | 2019 |
| Falke Financial Planning Ltd | Professional & scientific | 2022 |
| Falling With Style Ltd | Transport & logistics | 2024 |