| EMJ Holdings Limited | Financial services | 2025 |
| Emlmarketing Ltd | Professional & scientific | 2026 |
| Emma and Art Ltd | Wholesale & retail | 2017 |
| Emma and Jane Draper Limited | Other services | 2018 |
| Emma Budda Make UP Ltd | Other services | 2024 |
| Emma Kate Farley Ltd | Professional & scientific | 2023 |
| Emma Langton Ltd | Professional & scientific | 2022 |
| Emma Louise Property Lets Ltd | Real estate | 2022 |
| Emma Mcneill Ltd | Administrative & support | 2026 |
| Emma-Kate Retail Ltd | Other services | 2001 |
| Emmerson Industrial Doors Limited | Extraterritorial | 2014 |
| Emmerson Street Management Limited | Real estate | 2021 |
| Emmtk Technology and Consulting Ltd | Wholesale & retail | 2021 |
| Empathrive Ltd | Professional & scientific | 2023 |
| Emperico Group Limited | Professional & scientific | 2025 |
| Emperors Wharf Ltd | Construction | 2024 |
| Emphatically Ltd | Professional & scientific | 2025 |
| Empower M365 Ltd | Information & communication | 2025 |
| Empower Mindz Ltd | Professional & scientific | 2026 |
| Empower Training & Development C.I.C. | Education | 2001 |
| Empower York CIC | Education | 2024 |
| Empoweredbi Ltd | Professional & scientific | 2017 |
| Emprise Records Limited | Arts & recreation | 2018 |
| Empty Quarter Events Ltd | Hospitality | 2021 |
| Empyreus Holdings Limited | Real estate | 2021 |
| Emrys Business Advisors Limited | Administrative & support | 2017 |
| Emsal Ltd | Hospitality | 2025 |
| Emsley Mavor Ltd | Real estate | 2019 |
| EN Pointe Ltd | Education | 2018 |
| Enable Digital Ltd | Information & communication | 2017 |
| Enablers of York Limited | Health & social care | 2021 |
| Enceladus AI Limited | Information & communication | 2020 |
| Enchanting Occasions Ltd | Arts & recreation | 2024 |
| Encompass AI Ltd | Education | 2024 |
| Endeavour House ( York) Management Company Limited | Real estate | 2020 |
| Endeavour People Development Limited | Education | 2019 |
| Endi Ltd | Information & communication | 2025 |
| Endsleigh Convent Management Company Limited | Households | 2025 |
| Endurance Estates Land Promotion Limited | Real estate | 2018 |
| Endure Brewing Co Ltd | Manufacturing | 2015 |
| Energize Audio Visual Ltd | Wholesale & retail | 2020 |
| Energy & Logistics Solutions Ltd | Transport & logistics | 2016 |
| Energy Auditing Ltd | Professional & scientific | 2024 |
| Energy B2B Limited | Wholesale & retail | 2016 |
| Energy-Led Designs Limited | Financial services | 2012 |
| Enflo Ltd | Administrative & support | 2025 |
| Engaged Creative Ltd | Professional & scientific | 2022 |
| Engineroom Sport Limited | Information & communication | 2021 |
| Enginix Ltd | Information & communication | 2026 |
| England Angling Limited | Wholesale & retail | 2001 |
| English in York Limited | Extraterritorial | 1980 |
| Enigma Creative Limited | Administrative & support | 2010 |
| Enrogen Limited | Manufacturing | 2005 |
| Ensbury Park Veterinary Practice Limited | Professional & scientific | 2008 |
| ENT Plus Aesthetics Ltd | Health & social care | 2025 |
| Entelechy Recruitment Group Ltd | Administrative & support | 2021 |
| Enterprise Services (York) Limited | Real estate | 1967 |
| Entire Retail Solutions Limited | Wholesale & retail | 2010 |
| Entyos Limited | Professional & scientific | 2011 |
| Envams Ltd | Professional & scientific | 2022 |
| Environmental Science and Technology Ltd | Professional & scientific | 2017 |
| Environmental Technologies Group Limited | Real estate | 2008 |
| Enviroscope Consulting Limited | Professional & scientific | 2013 |
| Envista Consulting Limited | Information & communication | 2009 |
| Enviva Management International Holdings, Limited | Professional & scientific | 2016 |
| Enviva Management UK, Limited | Wholesale & retail | 2016 |
| Envoy Recruitment Ltd | Extraterritorial | 2000 |
| Envoyo Limited | Information & communication | 2021 |
| Envy York Ltd | Other services | 2013 |
| Enzo Accountants Limited | Professional & scientific | 2024 |
| EO Social Work Consultancy Ltd | Health & social care | 2022 |
| EOS Ventures Limited | Wholesale & retail | 2016 |
| EPC Seascape Ltd | Real estate | 2025 |
| EPH York Ltd | Construction | 2025 |
| Epione Health Ltd | Health & social care | 2022 |
| Epione Medical Limited | Health & social care | 2025 |
| EPM Innovative Construction Ltd | Construction | 2025 |
| Epos Distributor Limited | Wholesale & retail | 2009 |
| EPS Training and Events Ltd | Other services | 2009 |
| EQ Exchange Ltd | Extraterritorial | 2025 |
| Equalita Healthcare Ltd | Manufacturing | 2021 |
| Equality Limited | Health & social care | 2026 |
| Equi Travel Safe Ltd | Manufacturing | 2016 |
| Equicon Europe Holding Ltd | Extraterritorial | 2018 |
| Equilibrium Accountants Ltd | Professional & scientific | 2013 |
| Equine and Livestock Insurance Company Limited (the) | Financial services | 1934 |
| Equine Nutrition UK Ltd | Agriculture & fishing | 2023 |
| Equinox Financial Planning Ltd | Financial services | 2006 |
| Equip Centre Limited | Wholesale & retail | 2014 |
| Equivet Limited | Professional & scientific | 2016 |
| ERC Plant Limited | Administrative & support | 2018 |
| ERC Trading Limited | Wholesale & retail | 2008 |
| Ereira Limited | Arts & recreation | 2015 |
| Eren Food Ltd | Hospitality | 2019 |
| Eresme Limited | Administrative & support | 2022 |
| ERF Barbers York Ltd | Other services | 2026 |
| Eridor Group Holdings Limited | Professional & scientific | 1972 |
| Eridor Investments Limited | Real estate | 1972 |
| Erivan Group Limited | Financial services | 2024 |
| Erivan Holdings Limited | Construction | 1947 |