| Hub FS Limited | Financial services | 2021 |
| Huntingford Holidays Ltd | Hospitality | 2019 |
| Hydrixon Tech Ltd | Energy | 2025 |
| Hyspeed Racing Limited | Extraterritorial | 2021 |
| Iain Sears Ltd | Administrative & support | 2024 |
| IAN Mcdermott Associates Ltd | Professional & scientific | 2017 |
| Impact Health & Fitness Ltd | Arts & recreation | 2020 |
| Impact Interiors Ltd | Construction | 2022 |
| Imperial Motion Limited | Professional & scientific | 2026 |
| Inglestone Construction Management Limited | Construction | 2008 |
| Ingwe Holdings Ltd | Real estate | 2019 |
| Inhayle Ltd | Manufacturing | 2025 |
| Inntricate Limited | Hospitality | 2019 |
| Inspire Therapeutic Services Ltd | Health & social care | 2018 |
| Inspire Therapy CIC | Health & social care | 2024 |
| Instant Marquees Events Ltd | Wholesale & retail | 2020 |
| Integer Waves Ltd | Education | 2025 |
| Integrated Building Services Consulting Limited | Professional & scientific | 2026 |
| International Teaching Seminars Ltd | Education | 2015 |
| Item Find Ltd | Extraterritorial | 2024 |
| Izza Used Car Limited | Wholesale & retail | 2025 |
| J. B. Young & Sons Ltd | Construction | 2008 |
| J.A.Bladwell & Co.Limited | Extraterritorial | 1960 |
| Jackson Support Services Ltd | Professional & scientific | 2019 |
| JB Publishing Limited | Information & communication | 2018 |
| JDC Facilities Management Ltd | Professional & scientific | 2020 |
| Jendaa Holdings Limited | Extraterritorial | 2013 |
| Jesiga Limited | Financial services | 2025 |
| JG Fitness Limited | Arts & recreation | 2019 |
| Jiggery Limited | Financial services | 2026 |
| JM2 Limited | Manufacturing | 2002 |
| John Willmott (Carpenters) Limited | Construction | 2010 |
| Jotcham Property Ltd | Construction | 2025 |
| Jowal Golden Retrievers Ltd | Agriculture & fishing | 2023 |
| JSL Contracting Limited | Professional & scientific | 2007 |
| Juby Design by Kirstie Ltd | Professional & scientific | 2018 |
| Justingredients Limited | Wholesale & retail | 2012 |
| JW Legal Consulting Limited | Administrative & support | 2013 |
| JW Works Limited | Professional & scientific | 2013 |
| K a Edwards Electrical Limited | Construction | 2004 |
| K D Thorpe Ltd | Administrative & support | 2016 |
| Kalibistro Ltd | Hospitality | 2025 |
| Katakirmer Ltd | Health & social care | 2021 |
| Katharine Knight Kinesiology Ltd | Health & social care | 2024 |
| Katharine Lady Berkeley Mews Limited | Households | 1987 |
| Katharine Lady Berkeley's School | Education | 2011 |
| Kemp Technology Limited | Professional & scientific | 2014 |
| Kenneth Bleaken Building Contractors Limited | Construction | 2003 |
| Keybridge House Management Company Limited | Real estate | 2016 |
| Kilcot Conservation Limited | Construction | 1982 |
| KIN Developments Ltd | Real estate | 2025 |
| Kings Barbers Ltd | Other services | 2018 |
| Kingswood Village Stores Limited | Wholesale & retail | 2017 |
| Kirkpatrick Developments Ltd | Construction | 2021 |
| Knight Corporation Limited | Construction | 2021 |
| Knight Reliability Ltd | Professional & scientific | 2022 |
| Kobe Group Ltd | Construction | 2020 |
| LA Tete Limited | Health & social care | 2026 |
| Laffinage DU Fromage Ltd | Hospitality | 2018 |
| Landl Cotswold Developments Limited | Construction | 2023 |
| Lane Fine Art Limited | Wholesale & retail | 1961 |
| Lazerindustries Limited | Information & communication | 2024 |
| Lazy Machine Ltd | Administrative & support | 2013 |
| Lean Synergies Ltd | Professional & scientific | 2010 |
| Legalis Limited | Administrative & support | 2010 |
| Less Partners Ltd | Hospitality | 2024 |
| Lever Group Ltd | Professional & scientific | 2023 |
| Lifeworks by RIO Limited | Other services | 2016 |
| Liftruck Services Limited | Manufacturing | 2008 |
| Light Heart Productions Ltd | Administrative & support | 2012 |
| Likos People Ltd | Professional & scientific | 2020 |
| Lodestar General Counsel Limited | Professional & scientific | 2013 |
| Logue Properties Limited | Real estate | 2025 |
| London Flock Company Limited | Hospitality | 1951 |
| Longhorn Transportation & Hire Ltd | Transport & logistics | 2025 |
| Loom Furniture Limited | Manufacturing | 2017 |
| Loop Music Management Limited | Information & communication | 2006 |
| Lovetts Way Orchard Limited | Households | 2025 |
| Loving Home Limited | Wholesale & retail | 2017 |
| Loxley Solicitors Limited | Professional & scientific | 2011 |
| Loxley Trust Corporation Limited | Professional & scientific | 2025 |
| Loxley’S Services LLP | | 2023 |
| Ludkin Consulting Limited | Information & communication | 2007 |
| LW IT Consultancy Ltd | Extraterritorial | 2020 |
| Lyley Apartments (Wotton-Under-Edge) Management Company Limited | Households | 1990 |
| LYM Accident Repair Limited | Wholesale & retail | 2021 |
| M & PB Holdings Limited | Real estate | 2021 |
| M J Clark Mobile Mechanic Ltd | Wholesale & retail | 2021 |
| M M R Automotive Limited | Wholesale & retail | 2012 |
| Macnamara Arts Ltd | Arts & recreation | 2019 |
| Macniven & Cameron Homes Limited | Construction | 1999 |
| Madlie Limited | Information & communication | 2024 |
| Mahoney Stirling Enterprises Ltd | Real estate | 2024 |
| Main Events Services Ltd | Hospitality | 2025 |
| Majorlift Holdings Limited | Professional & scientific | 2002 |
| Majorlift Hydraulic Equipment Limited | Manufacturing | 1978 |
| Make the Most Limited | Information & communication | 2024 |
| Maple (392) Limited | Households | 2015 |
| Marijana Lawton Talent Partners Ltd | Administrative & support | 2025 |
| MARKETPLACE5 Ltd | Households | 2026 |