| Ella Stevens Racing Ltd | Arts & recreation | 2026 |
| Elmridge Construction Ltd | Construction | 2024 |
| Entropic Engineering Ltd | Professional & scientific | 2015 |
| Environmental Water Solutions Limited | Water & waste | 2003 |
| Envirostrat Limited | Professional & scientific | 2002 |
| Esti Software Ltd | Information & communication | 2016 |
| Estudio Londres Ltd | Professional & scientific | 2012 |
| Euraccess Holdings Ltd | Financial services | 2016 |
| Evergreen (Charfield) Limited | Real estate | 2020 |
| Evergreen Computing Limited | Information & communication | 1999 |
| EVH Social Care Consultancy Limited | Health & social care | 2015 |
| Exceliatis Limited | Real estate | 2026 |
| Exhibit One Limited | Information & communication | 2007 |
| Exotic Delights 1 Limited | Wholesale & retail | 2025 |
| Falco Golf Ltd | Manufacturing | 2026 |
| Farida Solutions Limited | Professional & scientific | 2004 |
| Farmer P Limited | Wholesale & retail | 2020 |
| Fatac Limited | Professional & scientific | 2009 |
| FCF Investments Limited | Agriculture & fishing | 2014 |
| Fgas Holdings Limited | Extraterritorial | 2013 |
| Fiona Jackson Consulting Limited | Professional & scientific | 2019 |
| Firing Close Holdings Limited | Real estate | 2016 |
| Fisher-Brown Partners Limited | Professional & scientific | 2026 |
| Fit for Women Ltd | Education | 2026 |
| Flavourbuzz Ltd | Wholesale & retail | 2018 |
| Flawless Spaces Bristol Limited | Administrative & support | 2021 |
| Fletcher Publishing Ltd | Information & communication | 2024 |
| Flobelle Property Ltd | Real estate | 2021 |
| Flossy Mcaslan Limited | Professional & scientific | 2026 |
| Flourishing Ltd | Administrative & support | 2018 |
| Fluxar Limited | Wholesale & retail | 2025 |
| Food Tech Innovation Ltd | Wholesale & retail | 2024 |
| Foxlow Developments Ltd | Hospitality | 2025 |
| Fragol Ltd | Information & communication | 2025 |
| Frederick Forbes Limited | Construction | 2010 |
| Fresh Development Group Ltd | Professional & scientific | 2012 |
| Fresh Professional Development Limited | Professional & scientific | 1999 |
| Frost Financial Ltd | Financial services | 2025 |
| FTC South West Limited | Real estate | 2003 |
| Fullstack Foundry Ltd | Information & communication | 2026 |
| Fuse Advisors Limited | Professional & scientific | 2022 |
| G & J Building and Renovations Ltd | Construction | 2019 |
| G.H.Wintle & SON Limited | Transport & logistics | 1962 |
| Garden & Home Improvements Ltd | Professional & scientific | 2012 |
| Garnett Solutions Ltd | Professional & scientific | 2024 |
| Gavin Morris (South West) Ltd | Construction | 2015 |
| GB Dibden General Builders Limited | Construction | 2012 |
| Gecko Craft Ltd | Wholesale & retail | 2020 |
| Genosave Ltd | Information & communication | 2026 |
| GEO Plexus Limited | Administrative & support | 2007 |
| Gibson Studios Limited | Arts & recreation | 2019 |
| Gill-Garcia Property Services Limited | Hospitality | 2013 |
| Gilly PUB Co Limited | Hospitality | 2023 |
| Glamping Cinema Ltd | Information & communication | 2020 |
| Gloucester House Mews Management Ltd. | Extraterritorial | 1989 |
| Gloucestershire Marques Company Ltd | Wholesale & retail | 2019 |
| Gloucestershire Swim School LLP | | 2014 |
| Go Laundry Services Limited | Other services | 2021 |
| Goauditme Limited | Information & communication | 2021 |
| Goggletek Ltd | Professional & scientific | 2020 |
| Goldwing Visions Limited | Wholesale & retail | 2024 |
| Goose Green Country Childcare Ltd | Health & social care | 2025 |
| Graycott Holdings Limited | Financial services | 2022 |
| Greaves Digital Development Services Ltd | Information & communication | 2025 |
| Greaves Solutions Limited | Information & communication | 2017 |
| Green Team One Ltd | Construction | 2022 |
| Greenbox Events Ltd | Water & waste | 2011 |
| Greenbox Events Trustees Limited | Other services | 2026 |
| Greens of Cheltenham Limited | Wholesale & retail | 1996 |
| Grounded Ltd | Education | 2013 |
| Growing Real Food for Nutrition CIC | Agriculture & fishing | 2020 |
| Guard Center UK Ltd | Construction | 2016 |
| HAL Messel Limited | Arts & recreation | 2024 |
| Hamptons Leisure Limited | Transport & logistics | 1990 |
| Hanney Estates Ltd | Real estate | 2023 |
| Happyhoundsbath Limited | Other services | 2016 |
| Hardwick Surveyors Ltd | Construction | 2021 |
| Harry Limes Ltd. | Hospitality | 1998 |
| Hatten Flooring Limited | Construction | 2026 |
| Hawker Hire Services Ltd | Transport & logistics | 2021 |
| Hawthorn HR Limited | Administrative & support | 2017 |
| Hawton Ltd | Hospitality | 2025 |
| HE Leisure Limited | Hospitality | 2021 |
| Healthy Energy Limited | Professional & scientific | 2014 |
| Heaven of the South Community Interest Company | Arts & recreation | 2015 |
| Henry Jones Social Mobility Consultancy Ltd | Professional & scientific | 2025 |
| Hi-Impact Cleaning Limited | Administrative & support | 2021 |
| Hideout Lounge & Bistro Ltd | Hospitality | 2020 |
| High Spirits (SW) Limited | Hospitality | 2012 |
| Hillesley Community PUB Limited | Hospitality | 2012 |
| Hillesley Holdings Ltd | Extraterritorial | 2018 |
| HIP Houzz Limited | Hospitality | 2016 |
| HJS Tech Consulting Ltd | Information & communication | 2022 |
| Holley Investments Limited | Financial services | 2025 |
| Home Ergonomics Ltd | Other services | 2026 |
| Honeyborne Holdings Ltd | Financial services | 2022 |
| Horkins Ltd | Real estate | 2026 |
| Horwood Farm Ltd | Other services | 2025 |
| Hotspots Limited | Real estate | 2003 |
| House of Elouvra Ltd | Wholesale & retail | 2026 |