| Enable Health Ltd | Health & social care | 2013 |
| Encompass Engineering (Witney) Ltd | Professional & scientific | 2014 |
| Enders-Uk Limited | Wholesale & retail | 2024 |
| Endlessly Unfolding Limited | Professional & scientific | 2026 |
| Endura Consultancy Limited | Professional & scientific | 2026 |
| ENE Engineering Limited | Professional & scientific | 2002 |
| Energy Property and Developments Ltd | Construction | 2018 |
| Energyrv Ltd | Administrative & support | 2019 |
| Enpolicy Consulting Ltd | Professional & scientific | 2025 |
| Enterprise Estates Limited | Real estate | 1960 |
| Enviroawards Holdings Limited | Real estate | 2024 |
| EOS Electrical Ltd | Construction | 2024 |
| EPA Trust | Education | 2012 |
| Epion Consulting Limited | Professional & scientific | 2020 |
| Epsilon Advisory Partners Ltd | Information & communication | 2019 |
| EPT Brickwork Ltd | Construction | 2026 |
| EPT Finance Limited | Financial services | 2023 |
| Equityfirst Ltd | Financial services | 2021 |
| Erank Limited | Wholesale & retail | 2011 |
| Eric Marchant & Co Limited | Real estate | 2007 |
| Erinyes Consulting Limited | Professional & scientific | 2015 |
| ERP RC Distribution Ltd | Wholesale & retail | 2025 |
| Esben Limited | Information & communication | 2021 |
| Escape Leisure Enterprises Ltd | Arts & recreation | 2011 |
| Escape Pool Bar Ltd | Arts & recreation | 2013 |
| Esophatec Ltd | Professional & scientific | 2024 |
| Essex Street Holdings Limited | Extraterritorial | 2023 |
| Estas Camshafts Limited | Wholesale & retail | 1999 |
| Estenna Limited | Wholesale & retail | 2023 |
| Estia Property Investments Limited | Real estate | 2021 |
| Estryx Pharma Limited | Professional & scientific | 2015 |
| ET Bpaa Services Ltd | Manufacturing | 2024 |
| Etch Associates Ltd | Professional & scientific | 2021 |
| Eton Place Residents Company Ltd. | Professional & scientific | 1991 |
| Euacquico 1 Limited | Wholesale & retail | 2021 |
| Euacquico 2 Limited | Wholesale & retail | 2021 |
| Euacquico 3 Limited | Wholesale & retail | 2021 |
| Euacquico 4 Limited | Wholesale & retail | 2021 |
| Euacquico 5 Limited | Wholesale & retail | 2021 |
| Euacquico 6 Limited | Wholesale & retail | 2021 |
| Eudii Ltd | Wholesale & retail | 2024 |
| Eumelia Ltd | Wholesale & retail | 2020 |
| Europe Asia Business Solutions Ltd. | Wholesale & retail | 2013 |
| European Partnership and Dialogue Limited | Professional & scientific | 2013 |
| Euroselection Limited | Professional & scientific | 1995 |
| Eutopia Productions Limited | Information & communication | 2015 |
| Evans Roofing & Maintenance Ltd | Construction | 2026 |
| Evenlode Court Management Limited | Administrative & support | 1994 |
| Evenlode Pathology Services Ltd | Health & social care | 2023 |
| Evenlode Technology Limited | Information & communication | 2026 |
| Event Fire Solutions Ltd | Public administration | 2011 |
| Eventsie Limited | Administrative & support | 2024 |
| Everest Court Limited | Real estate | 1953 |
| Eversco Limited | Financial services | 2026 |
| Everybody Chiropractic Limited | Health & social care | 2024 |
| Everyday Muses Jewellery Ltd | Wholesale & retail | 2024 |
| Evidence-Based Networks Limited | Information & communication | 2013 |
| Evie Willow Holdings Limited | Financial services | 2021 |
| Evie Willow Limited | Manufacturing | 2013 |
| Evolution Projects Ltd | Professional & scientific | 1993 |
| Evolve Hair Studio Limited | Other services | 2010 |
| Evora Design Consultancy Limited | Professional & scientific | 2018 |
| Evoralis Limited | Professional & scientific | 2021 |
| Evoramonte Investments Ltd | Real estate | 2020 |
| Ewan Norrie Coaching Limited | Other services | 2007 |
| Ewanme Film Ltd | Information & communication | 2022 |
| Ewanme Ltd | Information & communication | 2015 |
| Ewarrior Global Ltd | Wholesale & retail | 2021 |
| EWJ Transport Ltd | Transport & logistics | 2020 |
| Ewkin Alternatives Ltd | Financial services | 2024 |
| Ewkin Investments Ltd | Financial services | 2014 |
| Ewkin Livestock Limited | Agriculture & fishing | 2011 |
| Ewkin Property Ltd | Real estate | 2024 |
| EWN Aviation Ltd | Transport & logistics | 2026 |
| Exact Design and Build Ltd | Construction | 2020 |
| Exact Development Holdings Limited | Financial services | 2026 |
| Excel Coach Partners Limited | | 1992 |
| Excelothene Limited | Construction | 2009 |
| Executive Athlete Club Ltd | Arts & recreation | 2023 |
| Executive Fire and Security Ltd | Administrative & support | 2024 |
| Executive Security Locksmiths (Holdings) Limited | Financial services | 2022 |
| Executive Security Ltd | Construction | 2023 |
| Exelio Ltd | Information & communication | 2024 |
| Exovia Ltd | Wholesale & retail | 2019 |
| Expert Financial Solutions Limited | Financial services | 2004 |
| Expert Wealth Management Limited | Extraterritorial | 2011 |
| Express Autocentres Limited | Wholesale & retail | 2023 |
| Express Travel Transport Limited | Transport & logistics | 2024 |
| Express Windscreens (Witney) Limited | Wholesale & retail | 2014 |
| Express Windscreens Ltd | Extraterritorial | 2025 |
| Extad Accountancy Services Limited | Professional & scientific | 2003 |
| Extractive Industry Geology Conferences | Other services | 2008 |
| EYE Division Limited | Information & communication | 2005 |
| Eynley Limited | Administrative & support | 2007 |
| Eynsham 46 Ltd | Real estate | 2016 |
| Eynsham Anaesthetics Limited | Health & social care | 2023 |
| Eynsham Fenestration Holdings Limited | Professional & scientific | 2015 |
| Eynsham Greenscape Gardens Ltd | Administrative & support | 2021 |
| Eynsham House Advisory Ltd | Professional & scientific | 2022 |
| Eynsham House Ltd | Financial services | 2018 |