| Elnoumeir Ltd | Health & social care | 2023 |
| ELO Wellbeing Ltd | Other services | 2020 |
| Elpizo Limited | Professional & scientific | 2004 |
| EM Healthcare Services Limited | Health & social care | 2022 |
| EM Property Maintenance Limited | Construction | 2024 |
| EM Psychology Ltd | Education | 2026 |
| Em's Stars Limited | Education | 2021 |
| EMC Architects Limited | Professional & scientific | 2020 |
| Emerald Green Associates Limited | Professional & scientific | 2017 |
| Emerald Management Contract Services (NW) Ltd | Professional & scientific | 2016 |
| Emerald Saffron Properties Ltd. | Real estate | 2025 |
| Emerson Heath Limited | Professional & scientific | 2020 |
| Emissioneering Limited | Manufacturing | 2026 |
| Emlec Building Services Ltd | Construction | 2025 |
| EMM Enterprises Ltd | Arts & recreation | 2014 |
| Emma Mathews Psychotherapy Ltd | Health & social care | 2025 |
| Emma V Gough Ltd | Wholesale & retail | 2023 |
| Emmeline Property Ltd | Real estate | 2026 |
| Emmesselle Property Services Ltd | Financial services | 2019 |
| Emmomedical Ltd | Health & social care | 2025 |
| Emotiome Ltd | Professional & scientific | 2025 |
| Empire Training Ltd | Education | 2019 |
| Empower Accounting & Finance Ltd | Professional & scientific | 2025 |
| Empower Family Law Ltd | Administrative & support | 2026 |
| Empoweredfit Ltd | Arts & recreation | 2017 |
| Empowerment Passport Ltd | Education | 2020 |
| EMS Boutique Ltd | Wholesale & retail | 2023 |
| EN3 Electrical Tech Ltd | Construction | 2026 |
| Enaid Consulting Limited | Professional & scientific | 2026 |
| Enchanted Drinks Co Ltd | Wholesale & retail | 2017 |
| Encrop Ltd | Agriculture & fishing | 2019 |
| Endeavour Financial Planning Ltd | Financial services | 2011 |
| Endeavour Games Ltd | Financial services | 2021 |
| Endeavour Mortgages Limited | Administrative & support | 2017 |
| Endevr Ltd | Information & communication | 2024 |
| Endless Curiosity Ltd | Information & communication | 2020 |
| ENE Electrical Services Ltd | Energy | 2014 |
| Energise Cycles CIC | Manufacturing | 2023 |
| Energy Compass Ltd | Professional & scientific | 2024 |
| Energy Link Direct Ltd | Construction | 2025 |
| Energy Management Training Ltd | Education | 2025 |
| Enerji Group Ltd | Construction | 2024 |
| Enex International Ltd | Professional & scientific | 2017 |
| Engen Manufacturing Limited | Professional & scientific | 2020 |
| Engen Services Limited | Professional & scientific | 2020 |
| Engineer One Limited | Administrative & support | 2006 |
| Engineering Wise Limited | Professional & scientific | 2021 |
| Englewood Investments Ltd | Real estate | 2024 |
| Enhanced Fitness Collective Ltd | Health & social care | 2026 |
| Enigmatrix Alpha Limited | Professional & scientific | 2020 |
| Enjen Limited | Professional & scientific | 2019 |
| Enjiplex Ltd | Wholesale & retail | 2025 |
| Enka (NW) Ltd | Hospitality | 2025 |
| Enquire Limited | Professional & scientific | 2024 |
| Enriched - Learning & Development Limited | Professional & scientific | 2017 |
| ENS Gateway Limited | Information & communication | 2025 |
| Ensign Property Services Limited | Real estate | 2016 |
| Enspec Technology Ltd | Professional & scientific | 1987 |
| Entehome PVT Ltd | Wholesale & retail | 2023 |
| Enthoven Consulting Ltd | Professional & scientific | 2024 |
| Entrust Remote Financial Services Assistance Ltd | Administrative & support | 2022 |
| Enviro Spore Probiotics Ltd | Professional & scientific | 2019 |
| Envirocell Limited | Professional & scientific | 2024 |
| Environmental Temperature Control Limited | Energy | 2008 |
| Environoise Consulting Limited | Professional & scientific | 2009 |
| EPC Controls Ltd | Administrative & support | 2010 |
| EPC Planning Limited | Professional & scientific | 2025 |
| Epic Art Story Ltd | Manufacturing | 2021 |
| Epic Wirral CIC | Education | 2025 |
| Epicrates Ltd | Wholesale & retail | 2020 |
| EPK Group Ltd | Financial services | 2026 |
| Eponine Patisserie Limited | Manufacturing | 2013 |
| Equfund (SPV1) Limited | Real estate | 2006 |
| Equfund (SPV2) Limited | Real estate | 2008 |
| Equfund (SPV3) Limited | Real estate | 2008 |
| Equfund (SPV4) Limited | Real estate | 2008 |
| Equilibrium North West CIC | Health & social care | 2014 |
| Equine First Services Limited | Financial services | 2016 |
| Equipaws Ltd | Agriculture & fishing | 2022 |
| Equisafety Ltd | Administrative & support | 2010 |
| ER Adhd Consultancy Ltd | Health & social care | 2022 |
| ER Diamond Nails Ltd | Other services | 2024 |
| ER Pang Wirral Takeaway Ltd | Hospitality | 2026 |
| Eraklis Developments Limited | Construction | 2004 |
| Erin Processing Ltd | Information & communication | 2026 |
| Erma Qazi Ltd | Health & social care | 2026 |
| Erudio Limited | Information & communication | 2004 |
| ERV Limited | Professional & scientific | 2025 |
| ES Property Group Ltd | Real estate | 2024 |
| ESA Eyes Ltd | Health & social care | 2024 |
| Esbeare Ltd. | Arts & recreation | 2023 |
| Escaping Average Ltd | Information & communication | 2025 |
| Esme Jewellery Limited | Extraterritorial | 2011 |
| Esme Ursula Ltd | Wholesale & retail | 2025 |
| ESS (Midlands and NW) Limited | Professional & scientific | 2019 |
| Essell Business Solutions Ltd | Professional & scientific | 2012 |
| Essence Dental Limited | Health & social care | 2019 |
| EST 14 Beauty Limited | Other services | 2018 |
| Estarfish Ltd | Construction | 2023 |
| Estuary House Limited | Real estate | 2026 |