| Energy Project Engineering Limited | Professional & scientific | 2025 |
| Energyield Ltd | Information & communication | 2025 |
| Enfl Ltd. | Professional & scientific | 2002 |
| Engineered Logic Limited | Professional & scientific | 2022 |
| Engineering 138 Limited | Professional & scientific | 2023 |
| Engineering Catalyst Limited | Administrative & support | 2011 |
| English Oak Estates Limited Liability Partnership | | 2008 |
| English Oak Projects Limited | Construction | 2015 |
| Engn Studios Ltd | Information & communication | 2023 |
| Engware Dynamics Limited | Information & communication | 1994 |
| Enhance Professional Services Limited | Professional & scientific | 2012 |
| Enhanced Digital Solutions Ltd | Wholesale & retail | 2017 |
| Enlog Solutions Limited | Professional & scientific | 2020 |
| Enodamus Ltd | Professional & scientific | 2018 |
| Enough Studio Limited | Professional & scientific | 2020 |
| Enriched Limited | Professional & scientific | 1999 |
| Enterprise Agility Coaching Limited | Information & communication | 2021 |
| Enterprise Investment Scheme Company Limited | Professional & scientific | 2003 |
| Entity One Limited | Information & communication | 2007 |
| Environment Controls Ltd | Water & waste | 2021 |
| Environmental Controls Group Limited | Water & waste | 2021 |
| Envizage Ltd. | Professional & scientific | 2004 |
| EOK Solutions Limited | Information & communication | 2010 |
| Epoxy Products Limited | Manufacturing | 1984 |
| Equate Actuarial Services UK Limited | Financial services | 2025 |
| Equinox GRP Limited | Manufacturing | 2023 |
| ER Project Services Limited | Professional & scientific | 2025 |
| Ericanne Limited | Construction | 2021 |
| Erinvale Place Management Company Limited | Households | 2009 |
| Eristotle Limited | Other services | 2020 |
| ERP Works Limited | Administrative & support | 2019 |
| Erpx Limited | Professional & scientific | 2025 |
| Escor Toys Limited | Real estate | 1946 |
| Esfandiari IT Services Ltd | Information & communication | 2021 |
| Eshu Programme & Project Management Services Limited | Information & communication | 2023 |
| ESI Properties Limited | Real estate | 2006 |
| Esime Limited | Health & social care | 2013 |
| Esja Properties Limited | Administrative & support | 2013 |
| Eskimo App Ltd | Information & communication | 2019 |
| Esma Software Consulting Limited | Information & communication | 2023 |
| Esoteric Technologies Limited | Information & communication | 2023 |
| Essarts Wood Limited | Manufacturing | 2022 |
| Essential Drug and Alcohol Services | Other services | 1990 |
| Estate Resources & Management Limited | Arts & recreation | 1991 |
| Estatecreate Limited | Information & communication | 2007 |
| ETG BIDCO1 Ltd | Administrative & support | 2021 |
| Ethical Educaitor CIC | Education | 2025 |
| Ethmira Limited | Manufacturing | 2025 |
| Etre Design Limited | Other services | 2006 |
| Eudoxus Consulting Limited | Professional & scientific | 2010 |
| Eureka Rfid Limited | Manufacturing | 1997 |
| Euro Drain Ltd | Financial services | 2023 |
| Euroffset Limited | Other services | 1970 |
| Euroleisure Arena Rides Ltd | Arts & recreation | 2012 |
| EV23 Ltd | Financial services | 2022 |
| Evalys Property Ltd | Real estate | 2018 |
| Evans Above Tree Care Ltd | Agriculture & fishing | 2017 |
| Evenly Limited | Wholesale & retail | 2020 |
| Everchange Consulting Ltd | Information & communication | 2018 |
| Eversley Management (Bournemouth) Limited | Households | 1991 |
| Evertest Consultants Ltd | Information & communication | 2022 |
| Everydaydev Ltd | Information & communication | 2021 |
| Everything Works Ltd | Financial services | 2022 |
| Evidence in Action Ltd | Health & social care | 2015 |
| Evie's Cabs Limited | Transport & logistics | 2025 |
| Evoluon Limited | Information & communication | 2012 |
| Evolution Solutions Ltd | Professional & scientific | 2025 |
| Evolve Business Therapy Ltd | Professional & scientific | 2022 |
| Evolve Design Consultants Limited | Professional & scientific | 2016 |
| Evolve Power Design Ltd | Professional & scientific | 2025 |
| Evrbilt Limited | Professional & scientific | 2018 |
| Exaqter Limited | Professional & scientific | 2025 |
| Executive Project Solutions Limited | Information & communication | 2025 |
| Expand Emea Ltd | Wholesale & retail | 2023 |
| Expedition Clinicians Ltd | Health & social care | 2025 |
| Expert CDM Limited | Professional & scientific | 2024 |
| Explored Media Ltd | Information & communication | 2016 |
| Expoangel Limited | Administrative & support | 2026 |
| Exsta Consult Limited | Information & communication | 2026 |
| Exta Labs Ltd | Information & communication | 2026 |
| Exygen Limited | Professional & scientific | 2024 |
| EYE Automotive Ltd | Wholesale & retail | 2018 |
| EYE in the Sky Drones CIC | Other services | 2025 |
| EYE LUV EWE Limited | Information & communication | 2004 |
| Eyris Fashion Limited | Wholesale & retail | 2025 |
| F King Creative Ltd. | Professional & scientific | 2014 |
| F T Electrical Limited | Construction | 2003 |
| F.C. Douch & SON (Funerals) Limited | Other services | 1964 |
| F.D.S. Waste Services Ltd. | Water & waste | 2004 |
| FAB4 Properties Limited | Real estate | 2015 |
| Faberdashery Ltd. | Wholesale & retail | 2011 |
| Facilities Development Services Limited | Administrative & support | 2002 |
| Faib - Mental Health (Training) Limited | Extraterritorial | 2007 |
| Fail Safe Limited | Wholesale & retail | 2026 |
| Fair Creation & Validation Ltd | Professional & scientific | 2026 |
| Fairchild Investments Limited | Financial services | 2018 |
| Fairfields Tax Limited | Professional & scientific | 2020 |
| Fairhope Ltd | Health & social care | 2009 |
| Fairmount Property Group Limited | Real estate | 2022 |
| Fairview Park Management Company Limited | Households | 1982 |